Fluorescence lifetime imaging with time-gated detection of hyaluronidase using a long lifetime azadioxatriangulenium (ADOTA) fluorophore

Rahul Chib, Sebastian Requena, Mark Mummert, Yuri M. Strzhemechny, Ignacy Gryczynski, Julian Borejdo, Zygmunt Gryczynski, Rafal Fudala

Research output: Contribution to journalArticleResearchpeer-review

1 Citation (Scopus)

Abstract

A fluorescence lifetime imaging probe with a long lifetime was used in combination with time-gating for the detection of hyaluronidase using hyaluronic acid as the probe template. This probe was developed by heavily labeling hyaluronic acid with long lifetime azadioxatriangulenium fluorophores (ADOTA). We used this probe to image hyaluronidase produced by DU-145 prostate cancer cells.

Original languageEnglish
Number of pages1
JournalMethods and Applications in Fluorescence
Volume4
Issue number4
DOIs
StatePublished - 17 Nov 2016

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Hyaluronoglucosaminidase
Fluorophores
Fluorescence
Imaging techniques
Hyaluronic acid
life (durability)
fluorescence
probes
Hyaluronic Acid
acids
Labeling
marking
templates
cancer
Cells

Cite this

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title = "Fluorescence lifetime imaging with time-gated detection of hyaluronidase using a long lifetime azadioxatriangulenium (ADOTA) fluorophore",
abstract = "A fluorescence lifetime imaging probe with a long lifetime was used in combination with time-gating for the detection of hyaluronidase using hyaluronic acid as the probe template. This probe was developed by heavily labeling hyaluronic acid with long lifetime azadioxatriangulenium fluorophores (ADOTA). We used this probe to image hyaluronidase produced by DU-145 prostate cancer cells.",
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Fluorescence lifetime imaging with time-gated detection of hyaluronidase using a long lifetime azadioxatriangulenium (ADOTA) fluorophore. / Chib, Rahul; Requena, Sebastian; Mummert, Mark; Strzhemechny, Yuri M.; Gryczynski, Ignacy; Borejdo, Julian; Gryczynski, Zygmunt; Fudala, Rafal.

In: Methods and Applications in Fluorescence, Vol. 4, No. 4, 17.11.2016.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Fluorescence lifetime imaging with time-gated detection of hyaluronidase using a long lifetime azadioxatriangulenium (ADOTA) fluorophore

AU - Chib, Rahul

AU - Requena, Sebastian

AU - Mummert, Mark

AU - Strzhemechny, Yuri M.

AU - Gryczynski, Ignacy

AU - Borejdo, Julian

AU - Gryczynski, Zygmunt

AU - Fudala, Rafal

PY - 2016/11/17

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U2 - 10.1088/2050-6120/4/4/047001

DO - 10.1088/2050-6120/4/4/047001

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VL - 4

JO - Methods and applications in fluorescence

JF - Methods and applications in fluorescence

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