Fluorescence lifetime imaging with time-gated detection of hyaluronidase using a long lifetime azadioxatriangulenium (ADOTA) fluorophore

Rahul Chib; Sebastian Requena; Mark Mummert; Yuri M. Strzhemechny; Ignacy Gryczynski; Julian Borejdo; Zygmunt Gryczynski; Rafal Fudala

doi:10.1088/2050-6120/4/4/047001

Fluorescence lifetime imaging with time-gated detection of hyaluronidase using a long lifetime azadioxatriangulenium (ADOTA) fluorophore

Rahul Chib, Sebastian Requena, Mark Mummert, Yuri M. Strzhemechny, Ignacy Gryczynski, Julian Borejdo, Zygmunt Gryczynski, Rafal Fudala

Microbiology, Immunology & Genetics

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

A fluorescence lifetime imaging probe with a long lifetime was used in combination with time-gating for the detection of hyaluronidase using hyaluronic acid as the probe template. This probe was developed by heavily labeling hyaluronic acid with long lifetime azadioxatriangulenium fluorophores (ADOTA). We used this probe to image hyaluronidase produced by DU-145 prostate cancer cells.

Original language	English
Pages (from-to)	47001
Number of pages	1
Journal	Methods and Applications in Fluorescence
Volume	4
Issue number	4
DOIs	https://doi.org/10.1088/2050-6120/4/4/047001
State	Published - 17 Nov 2016

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Chib, Rahul ; Requena, Sebastian ; Mummert, Mark ; Strzhemechny, Yuri M. ; Gryczynski, Ignacy ; Borejdo, Julian ; Gryczynski, Zygmunt ; Fudala, Rafal. / Fluorescence lifetime imaging with time-gated detection of hyaluronidase using a long lifetime azadioxatriangulenium (ADOTA) fluorophore. In: Methods and Applications in Fluorescence. 2016 ; Vol. 4, No. 4. pp. 47001.

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abstract = "A fluorescence lifetime imaging probe with a long lifetime was used in combination with time-gating for the detection of hyaluronidase using hyaluronic acid as the probe template. This probe was developed by heavily labeling hyaluronic acid with long lifetime azadioxatriangulenium fluorophores (ADOTA). We used this probe to image hyaluronidase produced by DU-145 prostate cancer cells.",

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Fluorescence lifetime imaging with time-gated detection of hyaluronidase using a long lifetime azadioxatriangulenium (ADOTA) fluorophore. / Chib, Rahul; Requena, Sebastian; Mummert, Mark; Strzhemechny, Yuri M.; Gryczynski, Ignacy; Borejdo, Julian; Gryczynski, Zygmunt; Fudala, Rafal.

In: Methods and Applications in Fluorescence, Vol. 4, No. 4, 17.11.2016, p. 47001.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Fluorescence lifetime imaging with time-gated detection of hyaluronidase using a long lifetime azadioxatriangulenium (ADOTA) fluorophore

AU - Chib, Rahul

AU - Requena, Sebastian

AU - Mummert, Mark

AU - Strzhemechny, Yuri M.

AU - Gryczynski, Ignacy

AU - Borejdo, Julian

AU - Gryczynski, Zygmunt

AU - Fudala, Rafal

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PY - 2016/11/17

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AB - A fluorescence lifetime imaging probe with a long lifetime was used in combination with time-gating for the detection of hyaluronidase using hyaluronic acid as the probe template. This probe was developed by heavily labeling hyaluronic acid with long lifetime azadioxatriangulenium fluorophores (ADOTA). We used this probe to image hyaluronidase produced by DU-145 prostate cancer cells.

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ER -

Fluorescence lifetime imaging with time-gated detection of hyaluronidase using a long lifetime azadioxatriangulenium (ADOTA) fluorophore

Abstract

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