Abstract
A fluorescence lifetime imaging probe with a long lifetime was used in combination with time-gating for the detection of hyaluronidase using hyaluronic acid as the probe template. This probe was developed by heavily labeling hyaluronic acid with long lifetime azadioxatriangulenium fluorophores (ADOTA). We used this probe to image hyaluronidase produced by DU-145 prostate cancer cells.
| Original language | English |
|---|---|
| Number of pages | 1 |
| Journal | Methods and Applications in Fluorescence |
| Volume | 4 |
| Issue number | 4 |
| DOIs | |
| State | Published - 17 Nov 2016 |
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Fluorescence lifetime imaging with time-gated detection of hyaluronidase using a long lifetime azadioxatriangulenium (ADOTA) fluorophore. / Chib, Rahul; Requena, Sebastian; Mummert, Mark; Strzhemechny, Yuri M.; Gryczynski, Ignacy; Borejdo, Julian; Gryczynski, Zygmunt; Fudala, Rafal.
In: Methods and Applications in Fluorescence, Vol. 4, No. 4, 17.11.2016.Research output: Contribution to journal › Article
TY - JOUR
T1 - Fluorescence lifetime imaging with time-gated detection of hyaluronidase using a long lifetime azadioxatriangulenium (ADOTA) fluorophore
AU - Chib, Rahul
AU - Requena, Sebastian
AU - Mummert, Mark
AU - Strzhemechny, Yuri M.
AU - Gryczynski, Ignacy
AU - Borejdo, Julian
AU - Gryczynski, Zygmunt
AU - Fudala, Rafal
PY - 2016/11/17
Y1 - 2016/11/17
N2 - A fluorescence lifetime imaging probe with a long lifetime was used in combination with time-gating for the detection of hyaluronidase using hyaluronic acid as the probe template. This probe was developed by heavily labeling hyaluronic acid with long lifetime azadioxatriangulenium fluorophores (ADOTA). We used this probe to image hyaluronidase produced by DU-145 prostate cancer cells.
AB - A fluorescence lifetime imaging probe with a long lifetime was used in combination with time-gating for the detection of hyaluronidase using hyaluronic acid as the probe template. This probe was developed by heavily labeling hyaluronic acid with long lifetime azadioxatriangulenium fluorophores (ADOTA). We used this probe to image hyaluronidase produced by DU-145 prostate cancer cells.
UR - http://www.scopus.com/inward/record.url?scp=85054459939&partnerID=8YFLogxK
U2 - 10.1088/2050-6120/4/4/047001
DO - 10.1088/2050-6120/4/4/047001
M3 - Article
C2 - 28192308
AN - SCOPUS:85054459939
VL - 4
JO - Methods and applications in fluorescence
JF - Methods and applications in fluorescence
SN - 2050-6120
IS - 4
ER -