Fluorescence lifetime imaging with time-gated detection of hyaluronidase using a long lifetime azadioxatriangulenium (ADOTA) fluorophore

Rahul Chib; Sebastian Requena; Mark Mummert; Yuri M. Strzhemechny; Ignacy Gryczynski; Julian Borejdo; Zygmunt Gryczynski; Rafal Fudala

doi:10.1088/2050-6120/4/4/047001

Fluorescence lifetime imaging with time-gated detection of hyaluronidase using a long lifetime azadioxatriangulenium (ADOTA) fluorophore

Rahul Chib, Sebastian Requena, Mark Mummert, Yuri M. Strzhemechny, Ignacy Gryczynski, Julian Borejdo, Zygmunt Gryczynski, Rafal Fudala

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A fluorescence lifetime imaging probe with a long lifetime was used in combination with time-gating for the detection of hyaluronidase using hyaluronic acid as the probe template. This probe was developed by heavily labeling hyaluronic acid with long lifetime azadioxatriangulenium fluorophores (ADOTA). We used this probe to image hyaluronidase produced by DU-145 prostate cancer cells.

Original language	English
Pages (from-to)	47001
Number of pages	1
Journal	Methods and Applications in Fluorescence
Volume	4
Issue number	4
DOIs	https://doi.org/10.1088/2050-6120/4/4/047001
State	Published - 17 Nov 2016

Access to Document

10.1088/2050-6120/4/4/047001

Cite this

@article{8fd84391871c4b37aa283e1b48ea219b,

title = "Fluorescence lifetime imaging with time-gated detection of hyaluronidase using a long lifetime azadioxatriangulenium (ADOTA) fluorophore",

abstract = "A fluorescence lifetime imaging probe with a long lifetime was used in combination with time-gating for the detection of hyaluronidase using hyaluronic acid as the probe template. This probe was developed by heavily labeling hyaluronic acid with long lifetime azadioxatriangulenium fluorophores (ADOTA). We used this probe to image hyaluronidase produced by DU-145 prostate cancer cells.",

author = "Rahul Chib and Sebastian Requena and Mark Mummert and Strzhemechny, {Yuri M.} and Ignacy Gryczynski and Julian Borejdo and Zygmunt Gryczynski and Rafal Fudala",

year = "2016",

month = nov,

day = "17",

doi = "10.1088/2050-6120/4/4/047001",

language = "English",

volume = "4",

pages = "47001",

journal = "Methods and applications in fluorescence",

issn = "2050-6120",

publisher = "IOP Publishing Ltd.",

number = "4",

}

TY - JOUR

T1 - Fluorescence lifetime imaging with time-gated detection of hyaluronidase using a long lifetime azadioxatriangulenium (ADOTA) fluorophore

AU - Chib, Rahul

AU - Requena, Sebastian

AU - Mummert, Mark

AU - Strzhemechny, Yuri M.

AU - Gryczynski, Ignacy

AU - Borejdo, Julian

AU - Gryczynski, Zygmunt

AU - Fudala, Rafal

PY - 2016/11/17

Y1 - 2016/11/17

N2 - A fluorescence lifetime imaging probe with a long lifetime was used in combination with time-gating for the detection of hyaluronidase using hyaluronic acid as the probe template. This probe was developed by heavily labeling hyaluronic acid with long lifetime azadioxatriangulenium fluorophores (ADOTA). We used this probe to image hyaluronidase produced by DU-145 prostate cancer cells.

AB - A fluorescence lifetime imaging probe with a long lifetime was used in combination with time-gating for the detection of hyaluronidase using hyaluronic acid as the probe template. This probe was developed by heavily labeling hyaluronic acid with long lifetime azadioxatriangulenium fluorophores (ADOTA). We used this probe to image hyaluronidase produced by DU-145 prostate cancer cells.

UR - http://www.scopus.com/inward/record.url?scp=85054459939&partnerID=8YFLogxK

U2 - 10.1088/2050-6120/4/4/047001

DO - 10.1088/2050-6120/4/4/047001

M3 - Article

C2 - 28192308

AN - SCOPUS:85054459939

VL - 4

SP - 47001

JO - Methods and applications in fluorescence

JF - Methods and applications in fluorescence

SN - 2050-6120

IS - 4

ER -

Fluorescence lifetime imaging with time-gated detection of hyaluronidase using a long lifetime azadioxatriangulenium (ADOTA) fluorophore

Abstract

Access to Document

Other files and links

Fingerprint

Cite this