Fluorescence lifetime imaging with time-gated detection of hyaluronidase using a long lifetime azadioxatriangulenium (ADOTA) fluorophore

Rahul Chib, Sebastian Requena, Mark Mummert, Yuri M. Strzhemechny, Ignacy Gryczynski, Julian Borejdo, Zygmunt Gryczynski, Rafal Fudala

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Abstract

A fluorescence lifetime imaging probe with a long lifetime was used in combination with time-gating for the detection of hyaluronidase using hyaluronic acid as the probe template. This probe was developed by heavily labeling hyaluronic acid with long lifetime azadioxatriangulenium fluorophores (ADOTA). We used this probe to image hyaluronidase produced by DU-145 prostate cancer cells.

Original languageEnglish
Number of pages1
JournalMethods and Applications in Fluorescence
Volume4
Issue number4
DOIs
StatePublished - 17 Nov 2016

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