Fluorescence intensity and anisotropy decay of the 4′,6-diamidino-2-phenylindole-DNA complex resulting from one-photon and two-photon excitation

Joseph R. Lakowicz; Ignancy Gryczynski

doi:10.1007/BF00867671

Fluorescence intensity and anisotropy decay of the 4′,6-diamidino-2-phenylindole-DNA complex resulting from one-photon and two-photon excitation

Joseph R. Lakowicz, Ignancy Gryczynski

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39 Scopus citations

Abstract

We examined the emission spectra, intensity decays, and anisotropy decays of the DNA-4′,6-diamidino-2-phenylindole (DAPI) complex resulting from one- and two-photon excitation of fluorescence. Similar lifetimes and correlation times were recovered from the frequency-domain data. However, the initial anisotropies of DAPI for one- and two-photon excitation revealed different angles between the absorption and the emission oscillators, 17.8 and 23.8°, respectively. This suggests the presence of two overlapping transitions in DAPI with different one- and two-photon cross sections for absorption.

Original language	English
Pages (from-to)	117-122
Number of pages	6
Journal	Journal of Fluorescence
Volume	2
Issue number	2
DOIs	https://doi.org/10.1007/BF00867671
State	Published - Jun 1992

Keywords

4′,6-Diamidino-2-phenylindole-DNA complex
anisotropy decay
fluorescence intensity
one- and two-photon excitation

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10.1007/BF00867671

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title = "Fluorescence intensity and anisotropy decay of the 4′,6-diamidino-2-phenylindole-DNA complex resulting from one-photon and two-photon excitation",

abstract = "We examined the emission spectra, intensity decays, and anisotropy decays of the DNA-4′,6-diamidino-2-phenylindole (DAPI) complex resulting from one- and two-photon excitation of fluorescence. Similar lifetimes and correlation times were recovered from the frequency-domain data. However, the initial anisotropies of DAPI for one- and two-photon excitation revealed different angles between the absorption and the emission oscillators, 17.8 and 23.8°, respectively. This suggests the presence of two overlapping transitions in DAPI with different one- and two-photon cross sections for absorption.",

keywords = "4′,6-Diamidino-2-phenylindole-DNA complex, anisotropy decay, fluorescence intensity, one- and two-photon excitation",

author = "Lakowicz, {Joseph R.} and Ignancy Gryczynski",

year = "1992",

month = jun,

doi = "10.1007/BF00867671",

language = "English",

volume = "2",

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journal = "Journal of Fluorescence",

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T1 - Fluorescence intensity and anisotropy decay of the 4′,6-diamidino-2-phenylindole-DNA complex resulting from one-photon and two-photon excitation

AU - Lakowicz, Joseph R.

AU - Gryczynski, Ignancy

PY - 1992/6

Y1 - 1992/6

N2 - We examined the emission spectra, intensity decays, and anisotropy decays of the DNA-4′,6-diamidino-2-phenylindole (DAPI) complex resulting from one- and two-photon excitation of fluorescence. Similar lifetimes and correlation times were recovered from the frequency-domain data. However, the initial anisotropies of DAPI for one- and two-photon excitation revealed different angles between the absorption and the emission oscillators, 17.8 and 23.8°, respectively. This suggests the presence of two overlapping transitions in DAPI with different one- and two-photon cross sections for absorption.

AB - We examined the emission spectra, intensity decays, and anisotropy decays of the DNA-4′,6-diamidino-2-phenylindole (DAPI) complex resulting from one- and two-photon excitation of fluorescence. Similar lifetimes and correlation times were recovered from the frequency-domain data. However, the initial anisotropies of DAPI for one- and two-photon excitation revealed different angles between the absorption and the emission oscillators, 17.8 and 23.8°, respectively. This suggests the presence of two overlapping transitions in DAPI with different one- and two-photon cross sections for absorption.

KW - 4′,6-Diamidino-2-phenylindole-DNA complex

KW - anisotropy decay

KW - fluorescence intensity

KW - one- and two-photon excitation

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AN - SCOPUS:0001439403

SN - 1053-0509

VL - 2

SP - 117

EP - 122

JO - Journal of Fluorescence

JF - Journal of Fluorescence

IS - 2

ER -

Fluorescence intensity and anisotropy decay of the 4′,6-diamidino-2-phenylindole-DNA complex resulting from one-photon and two-photon excitation

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Keywords

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