Fluorescence intensity and anisotropy decay of the 4′,6-diamidino-2-phenylindole-DNA complex resulting from one-photon and two-photon excitation

Joseph R. Lakowicz, Ignancy Gryczynski

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

We examined the emission spectra, intensity decays, and anisotropy decays of the DNA-4′,6-diamidino-2-phenylindole (DAPI) complex resulting from one- and two-photon excitation of fluorescence. Similar lifetimes and correlation times were recovered from the frequency-domain data. However, the initial anisotropies of DAPI for one- and two-photon excitation revealed different angles between the absorption and the emission oscillators, 17.8 and 23.8°, respectively. This suggests the presence of two overlapping transitions in DAPI with different one- and two-photon cross sections for absorption.

Original languageEnglish
Pages (from-to)117-122
Number of pages6
JournalJournal of Fluorescence
Volume2
Issue number2
DOIs
StatePublished - 1 Jun 1992

Keywords

  • 4′,6-Diamidino-2-phenylindole-DNA complex
  • anisotropy decay
  • fluorescence intensity
  • one- and two-photon excitation

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