Abstract
We examined the emission spectra, intensity decays, and anisotropy decays of the DNA-4′,6-diamidino-2-phenylindole (DAPI) complex resulting from one- and two-photon excitation of fluorescence. Similar lifetimes and correlation times were recovered from the frequency-domain data. However, the initial anisotropies of DAPI for one- and two-photon excitation revealed different angles between the absorption and the emission oscillators, 17.8 and 23.8°, respectively. This suggests the presence of two overlapping transitions in DAPI with different one- and two-photon cross sections for absorption.
Original language | English |
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Pages (from-to) | 117-122 |
Number of pages | 6 |
Journal | Journal of Fluorescence |
Volume | 2 |
Issue number | 2 |
DOIs | |
State | Published - Jun 1992 |
Keywords
- 4′,6-Diamidino-2-phenylindole-DNA complex
- anisotropy decay
- fluorescence intensity
- one- and two-photon excitation