Fluorescence enhancements on silver colloid coated surfaces

Joanna Lukomska; Joanna Malicka; Ignacy Gryczynski; Joseph R. Lakowicz

doi:10.1023/B:JOFL.0000031823.04926.75

Fluorescence enhancements on silver colloid coated surfaces

Joanna Lukomska, Joanna Malicka, Ignacy Gryczynski, Joseph R. Lakowicz

Research output: Contribution to journal › Review article › peer-review

60 Scopus citations

Abstract

We observed a strong, more than 16-fold, enhancement of Texas Red-labeled BSA fluorescence emission when deposited on silver colloid coated surfaces (SCCS). The same labeled protein deposited on silver island films (SIFs) showed an approximate 8-fold fluorescence enhancement. The lifetimes of Texas Red-BSA fluorescence are significantly shorter on silvered surfaces than on uncoated quartz substrate indicating a strong change in radiative decay rate of the dyes. We also observed a 36-fold increased brightness of overlabeled fluorescein-HSA deposited on silver colloid coated surface. Stronger enhancement observed for overlabeled Fl-HSA protein indicates that presence of silver particles partially decreased self-quenching. Our results indicate that surfaces coated with silver colloids are valuable substrates for metal-enhanced fluorescence.

Original language	English
Pages (from-to)	417-423
Number of pages	7
Journal	Journal of Fluorescence
Volume	14
Issue number	4
DOIs	https://doi.org/10.1023/B:JOFL.0000031823.04926.75
State	Published - Jul 2004

Keywords

Colloids
Metal-enhanced fluorescence
Silver island films

Access to Document

10.1023/B:JOFL.0000031823.04926.75

Cite this

@article{e303bb3e564648f1a8b73de8fc0f1b0e,

title = "Fluorescence enhancements on silver colloid coated surfaces",

abstract = "We observed a strong, more than 16-fold, enhancement of Texas Red-labeled BSA fluorescence emission when deposited on silver colloid coated surfaces (SCCS). The same labeled protein deposited on silver island films (SIFs) showed an approximate 8-fold fluorescence enhancement. The lifetimes of Texas Red-BSA fluorescence are significantly shorter on silvered surfaces than on uncoated quartz substrate indicating a strong change in radiative decay rate of the dyes. We also observed a 36-fold increased brightness of overlabeled fluorescein-HSA deposited on silver colloid coated surface. Stronger enhancement observed for overlabeled Fl-HSA protein indicates that presence of silver particles partially decreased self-quenching. Our results indicate that surfaces coated with silver colloids are valuable substrates for metal-enhanced fluorescence.",

keywords = "Colloids, Metal-enhanced fluorescence, Silver island films",

author = "Joanna Lukomska and Joanna Malicka and Ignacy Gryczynski and Lakowicz, {Joseph R.}",

note = "Funding Information: This work was supported by NIH National Center for Research Resource, RR-08119, the Human Genome Institute, HG-002655, and from the National Institute for Bioimaging and Bioengineering, NIH-EB00682 and EB-00980.",

year = "2004",

month = jul,

doi = "10.1023/B:JOFL.0000031823.04926.75",

language = "English",

volume = "14",

pages = "417--423",

journal = "Journal of Fluorescence",

issn = "1053-0509",

publisher = "Springer New York",

number = "4",

}

TY - JOUR

T1 - Fluorescence enhancements on silver colloid coated surfaces

AU - Lukomska, Joanna

AU - Malicka, Joanna

AU - Gryczynski, Ignacy

AU - Lakowicz, Joseph R.

N1 - Funding Information: This work was supported by NIH National Center for Research Resource, RR-08119, the Human Genome Institute, HG-002655, and from the National Institute for Bioimaging and Bioengineering, NIH-EB00682 and EB-00980.

PY - 2004/7

Y1 - 2004/7

N2 - We observed a strong, more than 16-fold, enhancement of Texas Red-labeled BSA fluorescence emission when deposited on silver colloid coated surfaces (SCCS). The same labeled protein deposited on silver island films (SIFs) showed an approximate 8-fold fluorescence enhancement. The lifetimes of Texas Red-BSA fluorescence are significantly shorter on silvered surfaces than on uncoated quartz substrate indicating a strong change in radiative decay rate of the dyes. We also observed a 36-fold increased brightness of overlabeled fluorescein-HSA deposited on silver colloid coated surface. Stronger enhancement observed for overlabeled Fl-HSA protein indicates that presence of silver particles partially decreased self-quenching. Our results indicate that surfaces coated with silver colloids are valuable substrates for metal-enhanced fluorescence.

AB - We observed a strong, more than 16-fold, enhancement of Texas Red-labeled BSA fluorescence emission when deposited on silver colloid coated surfaces (SCCS). The same labeled protein deposited on silver island films (SIFs) showed an approximate 8-fold fluorescence enhancement. The lifetimes of Texas Red-BSA fluorescence are significantly shorter on silvered surfaces than on uncoated quartz substrate indicating a strong change in radiative decay rate of the dyes. We also observed a 36-fold increased brightness of overlabeled fluorescein-HSA deposited on silver colloid coated surface. Stronger enhancement observed for overlabeled Fl-HSA protein indicates that presence of silver particles partially decreased self-quenching. Our results indicate that surfaces coated with silver colloids are valuable substrates for metal-enhanced fluorescence.

KW - Colloids

KW - Metal-enhanced fluorescence

KW - Silver island films

UR - http://www.scopus.com/inward/record.url?scp=3042855392&partnerID=8YFLogxK

U2 - 10.1023/B:JOFL.0000031823.04926.75

DO - 10.1023/B:JOFL.0000031823.04926.75

M3 - Review article

C2 - 15617384

AN - SCOPUS:3042855392

SN - 1053-0509

VL - 14

SP - 417

EP - 423

JO - Journal of Fluorescence

JF - Journal of Fluorescence

IS - 4

ER -

Fluorescence enhancements on silver colloid coated surfaces

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this