Fluorescence enhancement on silver nanostructures

Studies of components of ribosomal translation in vitro

Wlodek Mandecki, Shashank Bharill, Julian Borejdo, Diana Cabral, Barry S. Cooperman, Ian Farrell, Linus Fetter, Emanuel Goldman, Zygmunt Gryczynski, Hieronim Jakubowski, Hanqing Liu, Rafal Luchowski, Evgenia Matveeva, Dongli Pan, Haiou Qin, Donald Tennant, Ignacy Gryczynski

Research output: Chapter in Book/Report/Conference proceedingConference contributionResearchpeer-review

4 Citations (Scopus)

Abstract

Metallic particles, silver in particular, can significantly enhance the fluorescence of dye molecules in the immediate vicinity (5-20 nm) of the particle. This magnifying effect can be theoretically explained/predicted by considering the change of photonic mode density near the fluorophore due to coupling to the conducting surface. We are using this method to observe fluorescence from a single ribosomal particle in a project aimed at acquiring sequence information from the translating ribosome (NIH's $1000 Genome Initiative). Several quartz slides with silver nanostructures were made using electron beam lithography techniques. The structures were approximately 50 nm high silver tiles measuring 400-700 nm on the side, and were spaced differently over a total area of 1 mm x 1 mm on any given quartz slide. In a preliminary experiment, we coated this surface with the Alexa 647-labeled antibodies and collected single molecule images using the MicroTime 200 (PicoQuant) confocal system. We showed that the fluorescence intensity measured over the silver islands film was more than 100-fold higher than fluorescence from a comparable site on uncoated section of the quartz slide. No noticeable photobleaching was seen. The fluorescence lifetime was very short, about 200 ps or less (this is the resolution limit of the system). The method has great promise for investigations of biologically relevant single molecules.

Original languageEnglish
Title of host publicationSingle Molecule Spectroscopy and Imaging
Volume6862
DOIs
StatePublished - 15 May 2008
EventSingle Molecule Spectroscopy and Imaging - San Jose, CA, United States
Duration: 19 Jan 200821 Jan 2008

Other

OtherSingle Molecule Spectroscopy and Imaging
CountryUnited States
CitySan Jose, CA
Period19/01/0821/01/08

Fingerprint

Nanostructures
Silver
Fluorescence
silver
Quartz
fluorescence
chutes
augmentation
quartz
Molecules
Optics and Photonics
ribosomes
Photobleaching
molecules
translating
Fluorophores
Electron beam lithography
genome
tiles
Tile

Keywords

  • Confocal
  • Fluorescence lifetime
  • Microscopic
  • Nanolithography
  • Photobleaching
  • Ribosome
  • Silver islands
  • Single molecule

Cite this

Mandecki, W., Bharill, S., Borejdo, J., Cabral, D., Cooperman, B. S., Farrell, I., ... Gryczynski, I. (2008). Fluorescence enhancement on silver nanostructures: Studies of components of ribosomal translation in vitro. In Single Molecule Spectroscopy and Imaging (Vol. 6862). [68620T] https://doi.org/10.1117/12.768612
Mandecki, Wlodek ; Bharill, Shashank ; Borejdo, Julian ; Cabral, Diana ; Cooperman, Barry S. ; Farrell, Ian ; Fetter, Linus ; Goldman, Emanuel ; Gryczynski, Zygmunt ; Jakubowski, Hieronim ; Liu, Hanqing ; Luchowski, Rafal ; Matveeva, Evgenia ; Pan, Dongli ; Qin, Haiou ; Tennant, Donald ; Gryczynski, Ignacy. / Fluorescence enhancement on silver nanostructures : Studies of components of ribosomal translation in vitro. Single Molecule Spectroscopy and Imaging. Vol. 6862 2008.
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abstract = "Metallic particles, silver in particular, can significantly enhance the fluorescence of dye molecules in the immediate vicinity (5-20 nm) of the particle. This magnifying effect can be theoretically explained/predicted by considering the change of photonic mode density near the fluorophore due to coupling to the conducting surface. We are using this method to observe fluorescence from a single ribosomal particle in a project aimed at acquiring sequence information from the translating ribosome (NIH's $1000 Genome Initiative). Several quartz slides with silver nanostructures were made using electron beam lithography techniques. The structures were approximately 50 nm high silver tiles measuring 400-700 nm on the side, and were spaced differently over a total area of 1 mm x 1 mm on any given quartz slide. In a preliminary experiment, we coated this surface with the Alexa 647-labeled antibodies and collected single molecule images using the MicroTime 200 (PicoQuant) confocal system. We showed that the fluorescence intensity measured over the silver islands film was more than 100-fold higher than fluorescence from a comparable site on uncoated section of the quartz slide. No noticeable photobleaching was seen. The fluorescence lifetime was very short, about 200 ps or less (this is the resolution limit of the system). The method has great promise for investigations of biologically relevant single molecules.",
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author = "Wlodek Mandecki and Shashank Bharill and Julian Borejdo and Diana Cabral and Cooperman, {Barry S.} and Ian Farrell and Linus Fetter and Emanuel Goldman and Zygmunt Gryczynski and Hieronim Jakubowski and Hanqing Liu and Rafal Luchowski and Evgenia Matveeva and Dongli Pan and Haiou Qin and Donald Tennant and Ignacy Gryczynski",
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Mandecki, W, Bharill, S, Borejdo, J, Cabral, D, Cooperman, BS, Farrell, I, Fetter, L, Goldman, E, Gryczynski, Z, Jakubowski, H, Liu, H, Luchowski, R, Matveeva, E, Pan, D, Qin, H, Tennant, D & Gryczynski, I 2008, Fluorescence enhancement on silver nanostructures: Studies of components of ribosomal translation in vitro. in Single Molecule Spectroscopy and Imaging. vol. 6862, 68620T, Single Molecule Spectroscopy and Imaging, San Jose, CA, United States, 19/01/08. https://doi.org/10.1117/12.768612

Fluorescence enhancement on silver nanostructures : Studies of components of ribosomal translation in vitro. / Mandecki, Wlodek; Bharill, Shashank; Borejdo, Julian; Cabral, Diana; Cooperman, Barry S.; Farrell, Ian; Fetter, Linus; Goldman, Emanuel; Gryczynski, Zygmunt; Jakubowski, Hieronim; Liu, Hanqing; Luchowski, Rafal; Matveeva, Evgenia; Pan, Dongli; Qin, Haiou; Tennant, Donald; Gryczynski, Ignacy.

Single Molecule Spectroscopy and Imaging. Vol. 6862 2008. 68620T.

Research output: Chapter in Book/Report/Conference proceedingConference contributionResearchpeer-review

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AU - Mandecki, Wlodek

AU - Bharill, Shashank

AU - Borejdo, Julian

AU - Cabral, Diana

AU - Cooperman, Barry S.

AU - Farrell, Ian

AU - Fetter, Linus

AU - Goldman, Emanuel

AU - Gryczynski, Zygmunt

AU - Jakubowski, Hieronim

AU - Liu, Hanqing

AU - Luchowski, Rafal

AU - Matveeva, Evgenia

AU - Pan, Dongli

AU - Qin, Haiou

AU - Tennant, Donald

AU - Gryczynski, Ignacy

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N2 - Metallic particles, silver in particular, can significantly enhance the fluorescence of dye molecules in the immediate vicinity (5-20 nm) of the particle. This magnifying effect can be theoretically explained/predicted by considering the change of photonic mode density near the fluorophore due to coupling to the conducting surface. We are using this method to observe fluorescence from a single ribosomal particle in a project aimed at acquiring sequence information from the translating ribosome (NIH's $1000 Genome Initiative). Several quartz slides with silver nanostructures were made using electron beam lithography techniques. The structures were approximately 50 nm high silver tiles measuring 400-700 nm on the side, and were spaced differently over a total area of 1 mm x 1 mm on any given quartz slide. In a preliminary experiment, we coated this surface with the Alexa 647-labeled antibodies and collected single molecule images using the MicroTime 200 (PicoQuant) confocal system. We showed that the fluorescence intensity measured over the silver islands film was more than 100-fold higher than fluorescence from a comparable site on uncoated section of the quartz slide. No noticeable photobleaching was seen. The fluorescence lifetime was very short, about 200 ps or less (this is the resolution limit of the system). The method has great promise for investigations of biologically relevant single molecules.

AB - Metallic particles, silver in particular, can significantly enhance the fluorescence of dye molecules in the immediate vicinity (5-20 nm) of the particle. This magnifying effect can be theoretically explained/predicted by considering the change of photonic mode density near the fluorophore due to coupling to the conducting surface. We are using this method to observe fluorescence from a single ribosomal particle in a project aimed at acquiring sequence information from the translating ribosome (NIH's $1000 Genome Initiative). Several quartz slides with silver nanostructures were made using electron beam lithography techniques. The structures were approximately 50 nm high silver tiles measuring 400-700 nm on the side, and were spaced differently over a total area of 1 mm x 1 mm on any given quartz slide. In a preliminary experiment, we coated this surface with the Alexa 647-labeled antibodies and collected single molecule images using the MicroTime 200 (PicoQuant) confocal system. We showed that the fluorescence intensity measured over the silver islands film was more than 100-fold higher than fluorescence from a comparable site on uncoated section of the quartz slide. No noticeable photobleaching was seen. The fluorescence lifetime was very short, about 200 ps or less (this is the resolution limit of the system). The method has great promise for investigations of biologically relevant single molecules.

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KW - Fluorescence lifetime

KW - Microscopic

KW - Nanolithography

KW - Photobleaching

KW - Ribosome

KW - Silver islands

KW - Single molecule

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SN - 9780819470379

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Mandecki W, Bharill S, Borejdo J, Cabral D, Cooperman BS, Farrell I et al. Fluorescence enhancement on silver nanostructures: Studies of components of ribosomal translation in vitro. In Single Molecule Spectroscopy and Imaging. Vol. 6862. 2008. 68620T https://doi.org/10.1117/12.768612