Fluorescence detection of the anti-cancer drug topotecan and related camptothecins in plasma and whole blood by two-photon excitation

Thomas G. Burke, Henryk M. Malak, Ignacy Gryczynski, Joseph R. Lakowicz

Research output: Contribution to journalConference articlepeer-review

Abstract

Recent FDA-approval of topotecan (9-dimethylaminomethyl-10- hydroxycamptothecin) and camptosar (CPT-11) along with the accelerated clinical development of related camptothecin drugs provides new hope for the successful treatment of human cancer, including neoplasms for which no effective treatments currently exist. Current clinical efforts worldwide are aimed at optimizing the therapeutic efficacies of the camptothecins, with the major focus on the determination of the most effective dosing schedules. To this end, technological advances which provide a direct and rapid means of measuring plasma drug levels (i.e. such that correlations between plasma drug levels and clinical responses can be sought) would be of great utility. Here we report on the direct fluorescence detection of topotecan and SN-38 in human plasma and topotecan in whole blood at micro molar levels using two-photon excitation at 730 or 820 nm. Topotecan was detected at concentrations as low as 0.05 and 1 (mu) M in plasma and whole blood, respectively. Since skin, blood and other tissues are translucent at long wavelengths, our results suggest the attractive possibility of homogeneous or noninvasive clinical sensing of camptothecins in situ using two-photon excitation.

Original languageEnglish
Pages (from-to)24-34
Number of pages11
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume2980
DOIs
StatePublished - 1 Dec 1997
EventAdvances in Fluorescence Sensing Technology III - San Jose, CA, United States
Duration: 9 Feb 19979 Feb 1997

Keywords

  • Anticancer
  • Blood
  • Camptothecin
  • Fluorescence
  • Near infrared
  • Phase-modulation
  • Time-resolved
  • Topoisomerase I
  • Topotecan
  • Two-photon excitation

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