Fluorescence detection of MMP-9. I. MMP-9 selectively cleaves Lys-Gly- Pro-Arg-Ser-Leu-Ser-Gly-Lys Peptide

Rafal Fudala; Amalendu P. Ranjan; Anindita Mukerjee; Jamboor K. Vishwanatha; Zygmunt Gryczynski; Julian Borejdo; Pabak Sarkar; Ignacy Gryczynski

doi:10.2174/138920111795470967

Fluorescence detection of MMP-9. I. MMP-9 selectively cleaves Lys-Gly- Pro-Arg-Ser-Leu-Ser-Gly-Lys Peptide

Rafal Fudala, Amalendu P. Ranjan, Anindita Mukerjee, Jamboor K. Vishwanatha, Zygmunt Gryczynski, Julian Borejdo, Pabak Sarkar, Ignacy Gryczynski

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22 Scopus citations

Abstract

MMP-9 enzyme recognizes a peptide sequence Lys-Gly-Pro-Arg-Ser-Leu-Ser-Gly-Lys and cleaves the peptide into two parts. We synthesized a dual fluorophore beacon consisting of 5-FAM and Cy5 dyes. The fluorescence emission of the fluorescein moiety is dramatically quenched by Cy5 molecule due to Förster Resonance Energy Transfer (FRET) and the fluorescence of Cy5 is strongly enhanced. Upon addition of MMP-9 enzyme, the fluorescence of 5-FAM intensifies and Cy5 decreases. The control MMP-2 enzyme does not cause any changes in either 5-FAM or Cy5 fluorescence. We believe that our observation will help in early detection of elevated MMP-9 levels under disease conditions.

Original language	English
Pages (from-to)	834-838
Number of pages	5
Journal	Current Pharmaceutical Biotechnology
Volume	12
Issue number	5
DOIs	https://doi.org/10.2174/138920111795470967
State	Published - Jun 2011

Keywords

FRET
Fluorescence
Labeled peptide
MMP-9

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title = "Fluorescence detection of MMP-9. I. MMP-9 selectively cleaves Lys-Gly- Pro-Arg-Ser-Leu-Ser-Gly-Lys Peptide",

abstract = "MMP-9 enzyme recognizes a peptide sequence Lys-Gly-Pro-Arg-Ser-Leu-Ser-Gly-Lys and cleaves the peptide into two parts. We synthesized a dual fluorophore beacon consisting of 5-FAM and Cy5 dyes. The fluorescence emission of the fluorescein moiety is dramatically quenched by Cy5 molecule due to F{\"o}rster Resonance Energy Transfer (FRET) and the fluorescence of Cy5 is strongly enhanced. Upon addition of MMP-9 enzyme, the fluorescence of 5-FAM intensifies and Cy5 decreases. The control MMP-2 enzyme does not cause any changes in either 5-FAM or Cy5 fluorescence. We believe that our observation will help in early detection of elevated MMP-9 levels under disease conditions.",

keywords = "FRET, Fluorescence, Labeled peptide, MMP-9",

author = "Rafal Fudala and Ranjan, {Amalendu P.} and Anindita Mukerjee and Vishwanatha, {Jamboor K.} and Zygmunt Gryczynski and Julian Borejdo and Pabak Sarkar and Ignacy Gryczynski",

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doi = "10.2174/138920111795470967",

language = "English",

volume = "12",

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T1 - Fluorescence detection of MMP-9. I. MMP-9 selectively cleaves Lys-Gly- Pro-Arg-Ser-Leu-Ser-Gly-Lys Peptide

AU - Fudala, Rafal

AU - Ranjan, Amalendu P.

AU - Mukerjee, Anindita

AU - Vishwanatha, Jamboor K.

AU - Gryczynski, Zygmunt

AU - Borejdo, Julian

AU - Sarkar, Pabak

AU - Gryczynski, Ignacy

PY - 2011/6

Y1 - 2011/6

N2 - MMP-9 enzyme recognizes a peptide sequence Lys-Gly-Pro-Arg-Ser-Leu-Ser-Gly-Lys and cleaves the peptide into two parts. We synthesized a dual fluorophore beacon consisting of 5-FAM and Cy5 dyes. The fluorescence emission of the fluorescein moiety is dramatically quenched by Cy5 molecule due to Förster Resonance Energy Transfer (FRET) and the fluorescence of Cy5 is strongly enhanced. Upon addition of MMP-9 enzyme, the fluorescence of 5-FAM intensifies and Cy5 decreases. The control MMP-2 enzyme does not cause any changes in either 5-FAM or Cy5 fluorescence. We believe that our observation will help in early detection of elevated MMP-9 levels under disease conditions.

AB - MMP-9 enzyme recognizes a peptide sequence Lys-Gly-Pro-Arg-Ser-Leu-Ser-Gly-Lys and cleaves the peptide into two parts. We synthesized a dual fluorophore beacon consisting of 5-FAM and Cy5 dyes. The fluorescence emission of the fluorescein moiety is dramatically quenched by Cy5 molecule due to Förster Resonance Energy Transfer (FRET) and the fluorescence of Cy5 is strongly enhanced. Upon addition of MMP-9 enzyme, the fluorescence of 5-FAM intensifies and Cy5 decreases. The control MMP-2 enzyme does not cause any changes in either 5-FAM or Cy5 fluorescence. We believe that our observation will help in early detection of elevated MMP-9 levels under disease conditions.

KW - FRET

KW - Fluorescence

KW - Labeled peptide

KW - MMP-9

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U2 - 10.2174/138920111795470967

DO - 10.2174/138920111795470967

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SN - 1389-2010

VL - 12

SP - 834

EP - 838

JO - Current Pharmaceutical Biotechnology

JF - Current Pharmaceutical Biotechnology

IS - 5

ER -

Fluorescence detection of MMP-9. I. MMP-9 selectively cleaves Lys-Gly- Pro-Arg-Ser-Leu-Ser-Gly-Lys Peptide

Abstract

Keywords

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