Fluorescence anisotropy imaging in drug discovery

Claudio Vinegoni; Paolo Fumene Feruglio; Ignacy Gryczynski; Ralph Mazitschek; Ralph Weissleder

doi:10.1016/j.addr.2018.01.019

Fluorescence anisotropy imaging in drug discovery

Claudio Vinegoni, Paolo Fumene Feruglio, Ignacy Gryczynski, Ralph Mazitschek, Ralph Weissleder

Microbiology, Immunology & Genetics

Research output: Contribution to journal › Article

6 Citations (Scopus)

Abstract

Non-invasive measurement of drug-target engagement can provide critical insights in the molecular pharmacology of small molecule drugs. Fluorescence polarization/fluorescence anisotropy measurements are commonly employed in protein/cell screening assays. However, the expansion of such measurements to the in vivo setting has proven difficult until recently. With the advent of high-resolution fluorescence anisotropy microscopy it is now possible to perform kinetic measurements of intracellular drug distribution and target engagement in commonly used mouse models. In this review we discuss the background, current advances and future perspectives in intravital fluorescence anisotropy measurements to derive pharmacokinetic and pharmacodynamic measurements in single cells and whole organs.

Original language	English
Journal	Advanced Drug Delivery Reviews
DOIs	https://doi.org/10.1016/j.addr.2018.01.019
State	Accepted/In press - 1 Jan 2018

Fingerprint

Fluorescence Polarization

Optical Imaging

Drug Discovery

Pharmaceutical Preparations

Fluorescence Microscopy

Pharmacokinetics

Pharmacology

Proteins

Keywords

Drug-target engagement
Fluorescence anisotropy imaging
Fluorescence polarization, fluorescence anisotropy
Fluorescently labeled drugs
Intravital imaging
Single-cell pharmacodynamics
Two photon microscopy

Cite this

Vinegoni, C., Feruglio, P. F., Gryczynski, I., Mazitschek, R., & Weissleder, R. (Accepted/In press). Fluorescence anisotropy imaging in drug discovery. Advanced Drug Delivery Reviews. https://doi.org/10.1016/j.addr.2018.01.019

Vinegoni, Claudio ; Feruglio, Paolo Fumene ; Gryczynski, Ignacy ; Mazitschek, Ralph ; Weissleder, Ralph. / Fluorescence anisotropy imaging in drug discovery. In: Advanced Drug Delivery Reviews. 2018.

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Vinegoni, C, Feruglio, PF, Gryczynski, I, Mazitschek, R & Weissleder, R 2018, 'Fluorescence anisotropy imaging in drug discovery', Advanced Drug Delivery Reviews. https://doi.org/10.1016/j.addr.2018.01.019

Fluorescence anisotropy imaging in drug discovery. / Vinegoni, Claudio; Feruglio, Paolo Fumene; Gryczynski, Ignacy; Mazitschek, Ralph; Weissleder, Ralph.

In: Advanced Drug Delivery Reviews, 01.01.2018.

Research output: Contribution to journal › Article

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