Fluorescence anisotropy controlled by light quenching

Research output: Contribution to journalConference article

Abstract

We demonstrated that fluorescence anisotropy can be effectively decreased or increased in the presence of light quenching, depending on relative polarizations of excitation and quenching pulses. For parallel light quenching anisotropy decreases to 0.103 and z-axis symmetry is being preserved. In the presence of perpendicular light quenching, the steady-state anisotropy of pyridine 2 glycerol solution increases from 0.368 for unquenched sample to 0.484, for quenched one. We show that angular distribution of transition moments loses the z-axis symmetry in the presence of perpendicular light quenching. In these cases we used more general definitions of anisotropy. Induced by light quenching anisotropy can be applied in both, steady-state and time-resolved measurements. In particular, the systems with low or none anisotropy can be investigated with proposed technique.

Original languageEnglish
Pages (from-to)40-50
Number of pages11
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume3256
DOIs
StatePublished - 1 Dec 1998
EventAdvances in Optical Biophysics - San Jose, CA, United States
Duration: 25 Jan 199826 Jan 1998

Fingerprint

Quenching
Fluorescence
Anisotropy
quenching
fluorescence
anisotropy
Perpendicular
Symmetry
Angular distribution
symmetry
glycerols
Time measurement
Glycerol
Pyridine
pyridines
Polarization
angular distribution
Excitation
time measurement
Moment

Cite this

@article{14b41501fed54bc0b3d11e374c084869,
title = "Fluorescence anisotropy controlled by light quenching",
abstract = "We demonstrated that fluorescence anisotropy can be effectively decreased or increased in the presence of light quenching, depending on relative polarizations of excitation and quenching pulses. For parallel light quenching anisotropy decreases to 0.103 and z-axis symmetry is being preserved. In the presence of perpendicular light quenching, the steady-state anisotropy of pyridine 2 glycerol solution increases from 0.368 for unquenched sample to 0.484, for quenched one. We show that angular distribution of transition moments loses the z-axis symmetry in the presence of perpendicular light quenching. In these cases we used more general definitions of anisotropy. Induced by light quenching anisotropy can be applied in both, steady-state and time-resolved measurements. In particular, the systems with low or none anisotropy can be investigated with proposed technique.",
author = "Ignacy Gryczynski and Zygmunt Gryczynski and Lakowicz, {Joseph R.}",
year = "1998",
month = "12",
day = "1",
doi = "10.1117/12.307076",
language = "English",
volume = "3256",
pages = "40--50",
journal = "Proceedings of SPIE - The International Society for Optical Engineering",
issn = "0277-786X",
publisher = "SPIE",

}

Fluorescence anisotropy controlled by light quenching. / Gryczynski, Ignacy; Gryczynski, Zygmunt; Lakowicz, Joseph R.

In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 3256, 01.12.1998, p. 40-50.

Research output: Contribution to journalConference article

TY - JOUR

T1 - Fluorescence anisotropy controlled by light quenching

AU - Gryczynski, Ignacy

AU - Gryczynski, Zygmunt

AU - Lakowicz, Joseph R.

PY - 1998/12/1

Y1 - 1998/12/1

N2 - We demonstrated that fluorescence anisotropy can be effectively decreased or increased in the presence of light quenching, depending on relative polarizations of excitation and quenching pulses. For parallel light quenching anisotropy decreases to 0.103 and z-axis symmetry is being preserved. In the presence of perpendicular light quenching, the steady-state anisotropy of pyridine 2 glycerol solution increases from 0.368 for unquenched sample to 0.484, for quenched one. We show that angular distribution of transition moments loses the z-axis symmetry in the presence of perpendicular light quenching. In these cases we used more general definitions of anisotropy. Induced by light quenching anisotropy can be applied in both, steady-state and time-resolved measurements. In particular, the systems with low or none anisotropy can be investigated with proposed technique.

AB - We demonstrated that fluorescence anisotropy can be effectively decreased or increased in the presence of light quenching, depending on relative polarizations of excitation and quenching pulses. For parallel light quenching anisotropy decreases to 0.103 and z-axis symmetry is being preserved. In the presence of perpendicular light quenching, the steady-state anisotropy of pyridine 2 glycerol solution increases from 0.368 for unquenched sample to 0.484, for quenched one. We show that angular distribution of transition moments loses the z-axis symmetry in the presence of perpendicular light quenching. In these cases we used more general definitions of anisotropy. Induced by light quenching anisotropy can be applied in both, steady-state and time-resolved measurements. In particular, the systems with low or none anisotropy can be investigated with proposed technique.

UR - http://www.scopus.com/inward/record.url?scp=0032225687&partnerID=8YFLogxK

U2 - 10.1117/12.307076

DO - 10.1117/12.307076

M3 - Conference article

AN - SCOPUS:0032225687

VL - 3256

SP - 40

EP - 50

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

SN - 0277-786X

ER -