Fluorescence Anisotropies of 4-Dimethylamino-a>- diphenylphosphinyl-fra/15-Styrene in Isotropic Media in the Case of One- and Two-Photon Excitation

A. Kawski, J. Gryczynski, Zygmunt Gryczynski

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Fluorescence emission anisotropies, r, and mean lifetimes of 4-dimethylamino-a>diphenylphosphinyl-rrans-styrene (DDPS) were measured in n-butanol at different temperatures from 20 to 80 °C for one- and two-photon excitation. When increasing the temperature, the lifetime decreased from 32 to 12 ps, the r values, however, remained constant (0.315 and 0.445 for one- and two-photon excitation, respectively) and were found to be temperature independent. For DDPS in glycerol at — 5°C, where rotational diffusion does not occur during the excited state lifetime, the limiting emission anisotropies were r0 0 f f { 0 can be due to vibrations performed after excitation by the DDPS molecule as a result of the initial shock.

Original languageEnglish
Pages (from-to)551-556
Number of pages6
JournalZeitschrift fur Naturforschung - Section A Journal of Physical Sciences
Issue number4
Publication statusPublished - 1 Apr 1993


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