Flow-induced regulation of brain endothelial cells in vitro

Xiao Ou Mao, Lin Xie, Rose B. Greenberg, Jack B. Greenberg, Botao Peng, Isabelle Mieling, Kunlin Jin, David A. Greenberg

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Endothelial cell (EC) function and susceptibility to vascular disease are regulated by flow; this relationship has been modeled in systemic, but not cerebrovascular, EC culture. We studied the effects of unidirectional flow of medium, produced by orbital rotation of cultures, on morphology and protein expression in bEnd.3 mouse brain ECs. Flow altered the expression of key transcription factors and gasotransmitter-synthesizing enzymes, and increased NO production. Statins and angiotensin receptor blockers reproduced the effect of flow on endothelial nitric oxide synthase expression. Thus, flow modified brain EC properties and function in vitro, with similarities and possible differences compared to previous studies on systemic ECs. Thus, the effect of flow on brain ECs can be modeled in vitro and may assist the investigation of mechanisms of cerebrovascular disease.

Original languageEnglish
Pages (from-to)82-87
Number of pages6
JournalVascular Pharmacology
Issue number2
StatePublished - Aug 2014


  • Angiotensin receptor blocker
  • Endothelial cell
  • Flow
  • Nitric oxide synthase
  • Statin


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