Fig1 facilitates calcium influx and localizes to membranes destined to undergo fusion during mating in Candida albicans

Meng Yang, Alexandra Brand, Srikantha Thyagarajan, Karla J. Daniels, David R. Soll, Neil A.R. Gow

Research output: Contribution to journalArticleResearchpeer-review

15 Citations (Scopus)

Abstract

Few mating-regulated genes have been characterized in Candida albicans. C. albicans FIG1 (CaFIG1) is a fungus-specific and mating-induced gene encoding a putative 4-transmembrane domain protein that shares sequence similarities with members of the claudin superfamily. In Saccharomyces cerevisiae, Fig1 is required for shmoo fusion and is upregulated in response to mating pheromones. Expression of CaFIG1 was also strongly activated in the presence of cells of the opposite mating type. CaFig1-green fluorescent protein (GFP) was visible only during the mating response, when it localized predominantly to the plasma membrane and perinuclear zone in mating projections and daughter cells. At the plasma membrane, CaFig1-GFP was visualized as discontinuous zones, but the distribution of perinuclear CaFig1-GFP was homogeneous. Exposure to pheromone induced a 5-fold increase in Ca2+ uptake in mating-competent opaque cells. Uptake was reduced substantially in the fig1Δ null mutant. CaFig1 is therefore involved in Ca2+ influx and localizes to membranes that are destined to undergo fusion during mating.

Original languageEnglish
Pages (from-to)435-444
Number of pages10
JournalEukaryotic Cell
Volume10
Issue number3
DOIs
StatePublished - 1 Mar 2011

Fingerprint

Green Fluorescent Proteins
Candida albicans
Pheromones
Calcium
Membranes
Cell Membrane
Genes
Saccharomyces cerevisiae
Fungi

Cite this

Yang, Meng ; Brand, Alexandra ; Thyagarajan, Srikantha ; Daniels, Karla J. ; Soll, David R. ; Gow, Neil A.R. / Fig1 facilitates calcium influx and localizes to membranes destined to undergo fusion during mating in Candida albicans. In: Eukaryotic Cell. 2011 ; Vol. 10, No. 3. pp. 435-444.
@article{b17773eeb7e1421c9d82ada39f6c8d4f,
title = "Fig1 facilitates calcium influx and localizes to membranes destined to undergo fusion during mating in Candida albicans",
abstract = "Few mating-regulated genes have been characterized in Candida albicans. C. albicans FIG1 (CaFIG1) is a fungus-specific and mating-induced gene encoding a putative 4-transmembrane domain protein that shares sequence similarities with members of the claudin superfamily. In Saccharomyces cerevisiae, Fig1 is required for shmoo fusion and is upregulated in response to mating pheromones. Expression of CaFIG1 was also strongly activated in the presence of cells of the opposite mating type. CaFig1-green fluorescent protein (GFP) was visible only during the mating response, when it localized predominantly to the plasma membrane and perinuclear zone in mating projections and daughter cells. At the plasma membrane, CaFig1-GFP was visualized as discontinuous zones, but the distribution of perinuclear CaFig1-GFP was homogeneous. Exposure to pheromone induced a 5-fold increase in Ca2+ uptake in mating-competent opaque cells. Uptake was reduced substantially in the fig1Δ null mutant. CaFig1 is therefore involved in Ca2+ influx and localizes to membranes that are destined to undergo fusion during mating.",
author = "Meng Yang and Alexandra Brand and Srikantha Thyagarajan and Daniels, {Karla J.} and Soll, {David R.} and Gow, {Neil A.R.}",
year = "2011",
month = "3",
day = "1",
doi = "10.1128/EC.00145-10",
language = "English",
volume = "10",
pages = "435--444",
journal = "Eukaryotic Cell",
issn = "1535-9778",
publisher = "American Society for Microbiology",
number = "3",

}

Fig1 facilitates calcium influx and localizes to membranes destined to undergo fusion during mating in Candida albicans. / Yang, Meng; Brand, Alexandra; Thyagarajan, Srikantha; Daniels, Karla J.; Soll, David R.; Gow, Neil A.R.

In: Eukaryotic Cell, Vol. 10, No. 3, 01.03.2011, p. 435-444.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Fig1 facilitates calcium influx and localizes to membranes destined to undergo fusion during mating in Candida albicans

AU - Yang, Meng

AU - Brand, Alexandra

AU - Thyagarajan, Srikantha

AU - Daniels, Karla J.

AU - Soll, David R.

AU - Gow, Neil A.R.

PY - 2011/3/1

Y1 - 2011/3/1

N2 - Few mating-regulated genes have been characterized in Candida albicans. C. albicans FIG1 (CaFIG1) is a fungus-specific and mating-induced gene encoding a putative 4-transmembrane domain protein that shares sequence similarities with members of the claudin superfamily. In Saccharomyces cerevisiae, Fig1 is required for shmoo fusion and is upregulated in response to mating pheromones. Expression of CaFIG1 was also strongly activated in the presence of cells of the opposite mating type. CaFig1-green fluorescent protein (GFP) was visible only during the mating response, when it localized predominantly to the plasma membrane and perinuclear zone in mating projections and daughter cells. At the plasma membrane, CaFig1-GFP was visualized as discontinuous zones, but the distribution of perinuclear CaFig1-GFP was homogeneous. Exposure to pheromone induced a 5-fold increase in Ca2+ uptake in mating-competent opaque cells. Uptake was reduced substantially in the fig1Δ null mutant. CaFig1 is therefore involved in Ca2+ influx and localizes to membranes that are destined to undergo fusion during mating.

AB - Few mating-regulated genes have been characterized in Candida albicans. C. albicans FIG1 (CaFIG1) is a fungus-specific and mating-induced gene encoding a putative 4-transmembrane domain protein that shares sequence similarities with members of the claudin superfamily. In Saccharomyces cerevisiae, Fig1 is required for shmoo fusion and is upregulated in response to mating pheromones. Expression of CaFIG1 was also strongly activated in the presence of cells of the opposite mating type. CaFig1-green fluorescent protein (GFP) was visible only during the mating response, when it localized predominantly to the plasma membrane and perinuclear zone in mating projections and daughter cells. At the plasma membrane, CaFig1-GFP was visualized as discontinuous zones, but the distribution of perinuclear CaFig1-GFP was homogeneous. Exposure to pheromone induced a 5-fold increase in Ca2+ uptake in mating-competent opaque cells. Uptake was reduced substantially in the fig1Δ null mutant. CaFig1 is therefore involved in Ca2+ influx and localizes to membranes that are destined to undergo fusion during mating.

UR - http://www.scopus.com/inward/record.url?scp=79952310218&partnerID=8YFLogxK

U2 - 10.1128/EC.00145-10

DO - 10.1128/EC.00145-10

M3 - Article

VL - 10

SP - 435

EP - 444

JO - Eukaryotic Cell

JF - Eukaryotic Cell

SN - 1535-9778

IS - 3

ER -