Extraction strategy for obtaining DNA from bloodstains for PCR amplification and typing of the HLA-DQa gene

Janet M. Jung, Catherine T. Comey, David B. Baer, Bruce Budowle

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

A simple, practical approach for the extraction of PCR-amplifiable DNA for the HLA-DQa gene from bloodstains deposited on various substrates is described. DNA from bloodstains is purified using Chelex 100 ion-exchange resin and then amplified. If amplification is not achieved, the extract is washed through a Centricon 100 dialysis/concentration tube. If the second amplification of this extract produces a negative result, the extract is processed with Chelex 100 again. This approach has been found to be reliable, safe, efficient and economical.

Original languageEnglish
Pages (from-to)145-148
Number of pages4
JournalInternational Journal of Legal Medicine
Volume104
Issue number3
DOIs
StatePublished - 1 May 1991

Keywords

  • DNA extraction
  • DQa
  • PCR

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