Extraction strategy for obtaining DNA from bloodstains for PCR amplification and typing of the HLA-DQa gene

Janet M. Jung; Catherine T. Comey; David B. Baer; Bruce Budowlel

doi:10.1007/BF01369719

Extraction strategy for obtaining DNA from bloodstains for PCR amplification and typing of the HLA-DQa gene

Janet M. Jung, Catherine T. Comey, David B. Baer, Bruce Budowlel

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

A simple, practical approach for the extraction of PCR-amplifiable DNA for the HLA-DQa gene from bloodstains deposited on various substrates is described. DNA from bloodstains is purified using Chelex 100 ion-exchange resin and then amplified. If amplification is not achieved, the extract is washed through a Centricon 100 dialysis/concentration tube. If the second amplification of this extract produces a negative result, the extract is processed with Chelex 100 again. This approach has been found to be reliable, safe, efficient and economical.

Original language	English
Pages (from-to)	145-148
Number of pages	4
Journal	International journal of legal medicine
Volume	104
Issue number	3
DOIs	https://doi.org/10.1007/BF01369719
State	Published - May 1991

Keywords

DNA extraction
DQa
PCR

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10.1007/BF01369719

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title = "Extraction strategy for obtaining DNA from bloodstains for PCR amplification and typing of the HLA-DQa gene",

abstract = "A simple, practical approach for the extraction of PCR-amplifiable DNA for the HLA-DQa gene from bloodstains deposited on various substrates is described. DNA from bloodstains is purified using Chelex 100 ion-exchange resin and then amplified. If amplification is not achieved, the extract is washed through a Centricon 100 dialysis/concentration tube. If the second amplification of this extract produces a negative result, the extract is processed with Chelex 100 again. This approach has been found to be reliable, safe, efficient and economical.",

keywords = "DNA extraction, DQa, PCR",

author = "Jung, {Janet M.} and Comey, {Catherine T.} and Baer, {David B.} and Bruce Budowlel",

year = "1991",

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T1 - Extraction strategy for obtaining DNA from bloodstains for PCR amplification and typing of the HLA-DQa gene

AU - Jung, Janet M.

AU - Comey, Catherine T.

AU - Baer, David B.

AU - Budowlel, Bruce

PY - 1991/5

Y1 - 1991/5

N2 - A simple, practical approach for the extraction of PCR-amplifiable DNA for the HLA-DQa gene from bloodstains deposited on various substrates is described. DNA from bloodstains is purified using Chelex 100 ion-exchange resin and then amplified. If amplification is not achieved, the extract is washed through a Centricon 100 dialysis/concentration tube. If the second amplification of this extract produces a negative result, the extract is processed with Chelex 100 again. This approach has been found to be reliable, safe, efficient and economical.

AB - A simple, practical approach for the extraction of PCR-amplifiable DNA for the HLA-DQa gene from bloodstains deposited on various substrates is described. DNA from bloodstains is purified using Chelex 100 ion-exchange resin and then amplified. If amplification is not achieved, the extract is washed through a Centricon 100 dialysis/concentration tube. If the second amplification of this extract produces a negative result, the extract is processed with Chelex 100 again. This approach has been found to be reliable, safe, efficient and economical.

KW - DNA extraction

KW - DQa

KW - PCR

UR - http://www.scopus.com/inward/record.url?scp=0025878517&partnerID=8YFLogxK

U2 - 10.1007/BF01369719

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M3 - Article

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EP - 148

JO - International Journal of Legal Medicine

JF - International Journal of Legal Medicine

IS - 3

ER -

Extraction strategy for obtaining DNA from bloodstains for PCR amplification and typing of the HLA-DQa gene

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Keywords

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