Abstract
A simple, practical approach for the extraction of PCR-amplifiable DNA for the HLA-DQa gene from bloodstains deposited on various substrates is described. DNA from bloodstains is purified using Chelex 100 ion-exchange resin and then amplified. If amplification is not achieved, the extract is washed through a Centricon 100 dialysis/concentration tube. If the second amplification of this extract produces a negative result, the extract is processed with Chelex 100 again. This approach has been found to be reliable, safe, efficient and economical.
Original language | English |
---|---|
Pages (from-to) | 145-148 |
Number of pages | 4 |
Journal | International journal of legal medicine |
Volume | 104 |
Issue number | 3 |
DOIs | |
State | Published - May 1991 |
Keywords
- DNA extraction
- DQa
- PCR