Extracellular vesicles extracted from young donor serum attenuate inflammaging via partially rejuvenating aged T-cell immunotolerance

Weikan Wang, Liefeng Wang, Linhui Ruan, Jiyoung Oh, Xiaowei Dong, Qichuan Zhuge, Dong Ming Su

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Abstract

Biologic aging results in a chronic inflammatory condition, termed inflammaging, which establishes a risk for such age-related diseases as neurocardiovascular diseases; therefore, it is of great importance to develop rejuvenation strategies that are able to attenuate inflammaging as ameans of intervention for age-relateddiseases.A promising rejuvenation factor that is present in young blood has been found that canmake aged neurons younger; however, the component in the young blood and its mechanism of action are poorly elucidated. We assessed rejuvenation in naturally aged mice with extracellular vesicles (EVs) or exosomes extracted from young murine serum on the basis of different spectrums of microRNAs in these vesicles from young and old sera.We found that EVs extracted from young donor mouse serum, rather than EVs extracted from old donor mouse serum or non-EV supernatant extracted from young donor mouse serum, were able to attenuate inflammaging in old mice. Inflammaging is attributed to multiple factors, one of which is thymic aging-released self-reactive T cell-induced pathology. We found that the attenuation of inflammaging after treatment with EVs from young serum partially contributed to the rejuvenation of thymic aging, which is characterized by partially reversed thymic involution, enhancementofnegative selectionsignals,andreducedautoreactions intheperiphery.Our resultsprovide evidence for understanding of the potential rejuvenation factor in the young donor serum,which holds great promise for the development of novel therapeutics to reduce morbidity and mortality caused by age-related inflammatory diseases.

Original languageEnglish
Pages (from-to)5899-5912
Number of pages14
JournalFASEB Journal
Volume32
Issue number11
DOIs
StatePublished - 1 Nov 2018

Fingerprint

T-cells
Rejuvenation
Aging of materials
T-Lymphocytes
Blood
Serum
Pathology
MicroRNAs
Neurons
Exosomes
Extracellular Vesicles
Morbidity
Mortality
Therapeutics

Keywords

  • Age-related thymic involution
  • Chronic inflammation
  • Exosomes
  • Rejuvenation
  • Young serum

Cite this

Wang, Weikan ; Wang, Liefeng ; Ruan, Linhui ; Oh, Jiyoung ; Dong, Xiaowei ; Zhuge, Qichuan ; Su, Dong Ming. / Extracellular vesicles extracted from young donor serum attenuate inflammaging via partially rejuvenating aged T-cell immunotolerance. In: FASEB Journal. 2018 ; Vol. 32, No. 11. pp. 5899-5912.
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Extracellular vesicles extracted from young donor serum attenuate inflammaging via partially rejuvenating aged T-cell immunotolerance. / Wang, Weikan; Wang, Liefeng; Ruan, Linhui; Oh, Jiyoung; Dong, Xiaowei; Zhuge, Qichuan; Su, Dong Ming.

In: FASEB Journal, Vol. 32, No. 11, 01.11.2018, p. 5899-5912.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Extracellular vesicles extracted from young donor serum attenuate inflammaging via partially rejuvenating aged T-cell immunotolerance

AU - Wang, Weikan

AU - Wang, Liefeng

AU - Ruan, Linhui

AU - Oh, Jiyoung

AU - Dong, Xiaowei

AU - Zhuge, Qichuan

AU - Su, Dong Ming

PY - 2018/11/1

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N2 - Biologic aging results in a chronic inflammatory condition, termed inflammaging, which establishes a risk for such age-related diseases as neurocardiovascular diseases; therefore, it is of great importance to develop rejuvenation strategies that are able to attenuate inflammaging as ameans of intervention for age-relateddiseases.A promising rejuvenation factor that is present in young blood has been found that canmake aged neurons younger; however, the component in the young blood and its mechanism of action are poorly elucidated. We assessed rejuvenation in naturally aged mice with extracellular vesicles (EVs) or exosomes extracted from young murine serum on the basis of different spectrums of microRNAs in these vesicles from young and old sera.We found that EVs extracted from young donor mouse serum, rather than EVs extracted from old donor mouse serum or non-EV supernatant extracted from young donor mouse serum, were able to attenuate inflammaging in old mice. Inflammaging is attributed to multiple factors, one of which is thymic aging-released self-reactive T cell-induced pathology. We found that the attenuation of inflammaging after treatment with EVs from young serum partially contributed to the rejuvenation of thymic aging, which is characterized by partially reversed thymic involution, enhancementofnegative selectionsignals,andreducedautoreactions intheperiphery.Our resultsprovide evidence for understanding of the potential rejuvenation factor in the young donor serum,which holds great promise for the development of novel therapeutics to reduce morbidity and mortality caused by age-related inflammatory diseases.

AB - Biologic aging results in a chronic inflammatory condition, termed inflammaging, which establishes a risk for such age-related diseases as neurocardiovascular diseases; therefore, it is of great importance to develop rejuvenation strategies that are able to attenuate inflammaging as ameans of intervention for age-relateddiseases.A promising rejuvenation factor that is present in young blood has been found that canmake aged neurons younger; however, the component in the young blood and its mechanism of action are poorly elucidated. We assessed rejuvenation in naturally aged mice with extracellular vesicles (EVs) or exosomes extracted from young murine serum on the basis of different spectrums of microRNAs in these vesicles from young and old sera.We found that EVs extracted from young donor mouse serum, rather than EVs extracted from old donor mouse serum or non-EV supernatant extracted from young donor mouse serum, were able to attenuate inflammaging in old mice. Inflammaging is attributed to multiple factors, one of which is thymic aging-released self-reactive T cell-induced pathology. We found that the attenuation of inflammaging after treatment with EVs from young serum partially contributed to the rejuvenation of thymic aging, which is characterized by partially reversed thymic involution, enhancementofnegative selectionsignals,andreducedautoreactions intheperiphery.Our resultsprovide evidence for understanding of the potential rejuvenation factor in the young donor serum,which holds great promise for the development of novel therapeutics to reduce morbidity and mortality caused by age-related inflammatory diseases.

KW - Age-related thymic involution

KW - Chronic inflammation

KW - Exosomes

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DO - 10.1096/fj.201800059R

M3 - Article

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JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

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