Expression of the RNA-binding protein TIAR is increased in neurons after ischemic cerebral injury

Kunlin Jin, Wenjin Li, Tetsuya Nagayama, Xiangjun He, Amy D. Sinor, Juni Chang, Xiaoou Mao, Steven H. Graham, Roger P. Simon, David A. Greenberg

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

T-cell restricted intracellular antigen-related protein (TIAR) is an RNA recognition motif-type RNA-binding protein that has been implicated in the apoptotic death of T-lymphocytes and retinal pigment epithelial cells. Western blots prepared with a monoclonal antibody against TIAR showed expression in normal rat hippocampus, and induction by 15 min of global cerebral ischemia. This increased expression was evident at 8 hr after ischemia and maximal at 24 hr, whereas expression at 72 hr was reduced below basal levels. Expression of TIAR protein was also increased in parietal cortex 6 and 24 hr after 90 min of focal cerebral ischemia induced by middle cerebral artery (MCA) occlusion, as well as in cultured cortical neurons and astroglia after exposure to hypoxia in vitro. Immunocytochemistry showed that increased expression of TIAR occurred mainly in the CA1 sector of hippocampus 24 hr after global ischemia, and in cortical and striatal neurons 24 hr after 20 or 90 min of focal ischemia. Double-labeling studies showed that TIAR protein expression was co-localized with DNA damage in neuronal cells. The findings suggest that TIAR may be involved in neuronal cell death after cerebral ischemic injury. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)767-774
Number of pages8
JournalJournal of Neuroscience Research
Volume59
Issue number6
DOIs
StatePublished - 15 Mar 2000

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RNA-Binding Proteins
T-Lymphocytes
Neurons
Antigens
Wounds and Injuries
Proteins
Ischemia
Brain Ischemia
Hippocampus
Corpus Striatum
Parietal Lobe
Retinal Pigments
Middle Cerebral Artery Infarction
Astrocytes
DNA Damage
Cell Death
Western Blotting
Epithelial Cells
Immunohistochemistry
Monoclonal Antibodies

Keywords

  • Brain
  • Cell death
  • Hypoxia
  • Ischemia
  • Protein expression
  • TIAR

Cite this

Jin, Kunlin ; Li, Wenjin ; Nagayama, Tetsuya ; He, Xiangjun ; Sinor, Amy D. ; Chang, Juni ; Mao, Xiaoou ; Graham, Steven H. ; Simon, Roger P. ; Greenberg, David A. / Expression of the RNA-binding protein TIAR is increased in neurons after ischemic cerebral injury. In: Journal of Neuroscience Research. 2000 ; Vol. 59, No. 6. pp. 767-774.
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Expression of the RNA-binding protein TIAR is increased in neurons after ischemic cerebral injury. / Jin, Kunlin; Li, Wenjin; Nagayama, Tetsuya; He, Xiangjun; Sinor, Amy D.; Chang, Juni; Mao, Xiaoou; Graham, Steven H.; Simon, Roger P.; Greenberg, David A.

In: Journal of Neuroscience Research, Vol. 59, No. 6, 15.03.2000, p. 767-774.

Research output: Contribution to journalArticle

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T1 - Expression of the RNA-binding protein TIAR is increased in neurons after ischemic cerebral injury

AU - Jin, Kunlin

AU - Li, Wenjin

AU - Nagayama, Tetsuya

AU - He, Xiangjun

AU - Sinor, Amy D.

AU - Chang, Juni

AU - Mao, Xiaoou

AU - Graham, Steven H.

AU - Simon, Roger P.

AU - Greenberg, David A.

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Y1 - 2000/3/15

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AB - T-cell restricted intracellular antigen-related protein (TIAR) is an RNA recognition motif-type RNA-binding protein that has been implicated in the apoptotic death of T-lymphocytes and retinal pigment epithelial cells. Western blots prepared with a monoclonal antibody against TIAR showed expression in normal rat hippocampus, and induction by 15 min of global cerebral ischemia. This increased expression was evident at 8 hr after ischemia and maximal at 24 hr, whereas expression at 72 hr was reduced below basal levels. Expression of TIAR protein was also increased in parietal cortex 6 and 24 hr after 90 min of focal cerebral ischemia induced by middle cerebral artery (MCA) occlusion, as well as in cultured cortical neurons and astroglia after exposure to hypoxia in vitro. Immunocytochemistry showed that increased expression of TIAR occurred mainly in the CA1 sector of hippocampus 24 hr after global ischemia, and in cortical and striatal neurons 24 hr after 20 or 90 min of focal ischemia. Double-labeling studies showed that TIAR protein expression was co-localized with DNA damage in neuronal cells. The findings suggest that TIAR may be involved in neuronal cell death after cerebral ischemic injury. (C) 2000 Wiley-Liss, Inc.

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