Expression of the proto-oncogene bcl-2 is increased in the rat brain following kainate-induced seizures

S. H. Graham, J. Chen, R. A. Stetler, R. L. Zhu, Kunlin Jin, R. P. Simon

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Abstract

The proto-oncogene bcl-2 is an important suppressor of apoptotic cell death in development and of both apoptotic and necrotic cell death in mature neurons. We studied expression of bcl-2 and the related gene, bax, which may promote cell death, after seizures induced by systemic kainate injection in rats. Expression of bcl-2 mRNA was studied by in situ hybridization. Bax and bcl-2 protein expression was studied by immunocytochemistry. Histologic analysis of cresyl violet-stained paraffin sections was performed at 72 h. bcl-2 protein was expressed in CA1 neurons, a region that is injured, yet survives after seizures. Bcl-2 mRNA was expressed in CA3, a region where there is extensive neuronal death at 72 h, but the bcl-2 protein was not translated. However, bax protein expression in CA3 was increased at 24 h. These results support a possible role for bcl-2 in promoting survival of CA3 after seizures.

Original languageEnglish
Pages (from-to)243-250
Number of pages8
JournalRestorative Neurology and Neuroscience
Volume9
Issue number4
StatePublished - 1 Jan 1996

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Keywords

  • Apoptosis
  • Epilepsy
  • Hippocampus
  • bcl-2
  • bcl-x

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