Expression of simian virus 40 large T antigen in Escherichia coli using vectors based on the regulatable rac promoter

J. M. Pistillo; J. K. Vishwanatha

doi:10.1016/0006-291X(90)92013-P

Expression of simian virus 40 large T antigen in Escherichia coli using vectors based on the regulatable rac promoter

J. M. Pistillo, J. K. Vishwanatha

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Abstract

Simian Virus 40 large T antigen is a multi-functional protein that is involved in the initiation of viral DNA replication, regulation of viral transcription and cell transformation. Bacterial expression vectors, pER23-1 and pER23-2, that are based on the regulatable rac promoter were used to produce T antigen either as a free protein or as a fusion protein. We have observed efficient transcription of the cloned T antigen gene in most of the recombinants. However, expression of the T antigen protein was inefficient and most of the expressed protein was truncated. This may be due to differences in codon usage in E. coli or to rapid protein degradation.

Original language	English
Pages (from-to)	1129-1137
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	169
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(90)92013-P
State	Published - 29 Jun 1990

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title = "Expression of simian virus 40 large T antigen in Escherichia coli using vectors based on the regulatable rac promoter",

abstract = "Simian Virus 40 large T antigen is a multi-functional protein that is involved in the initiation of viral DNA replication, regulation of viral transcription and cell transformation. Bacterial expression vectors, pER23-1 and pER23-2, that are based on the regulatable rac promoter were used to produce T antigen either as a free protein or as a fusion protein. We have observed efficient transcription of the cloned T antigen gene in most of the recombinants. However, expression of the T antigen protein was inefficient and most of the expressed protein was truncated. This may be due to differences in codon usage in E. coli or to rapid protein degradation.",

author = "Pistillo, {J. M.} and Vishwanatha, {J. K.}",

note = "Funding Information: This research was supported by a seed grant (#89-62) from the University of Nebraska Medical Center to JKV. We wish to thank Dr. Solon Rhode for helpful discussions during this project and Drs. Stanley Cox and William Chaney for critically reviewing this manuscript. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1990",

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doi = "10.1016/0006-291X(90)92013-P",

language = "English",

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T1 - Expression of simian virus 40 large T antigen in Escherichia coli using vectors based on the regulatable rac promoter

AU - Pistillo, J. M.

AU - Vishwanatha, J. K.

N1 - Funding Information: This research was supported by a seed grant (#89-62) from the University of Nebraska Medical Center to JKV. We wish to thank Dr. Solon Rhode for helpful discussions during this project and Drs. Stanley Cox and William Chaney for critically reviewing this manuscript. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1990/6/29

Y1 - 1990/6/29

N2 - Simian Virus 40 large T antigen is a multi-functional protein that is involved in the initiation of viral DNA replication, regulation of viral transcription and cell transformation. Bacterial expression vectors, pER23-1 and pER23-2, that are based on the regulatable rac promoter were used to produce T antigen either as a free protein or as a fusion protein. We have observed efficient transcription of the cloned T antigen gene in most of the recombinants. However, expression of the T antigen protein was inefficient and most of the expressed protein was truncated. This may be due to differences in codon usage in E. coli or to rapid protein degradation.

AB - Simian Virus 40 large T antigen is a multi-functional protein that is involved in the initiation of viral DNA replication, regulation of viral transcription and cell transformation. Bacterial expression vectors, pER23-1 and pER23-2, that are based on the regulatable rac promoter were used to produce T antigen either as a free protein or as a fusion protein. We have observed efficient transcription of the cloned T antigen gene in most of the recombinants. However, expression of the T antigen protein was inefficient and most of the expressed protein was truncated. This may be due to differences in codon usage in E. coli or to rapid protein degradation.

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Expression of simian virus 40 large T antigen in Escherichia coli using vectors based on the regulatable rac promoter

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