Expression of ICAM-1 in implanted primary and metastatic squamous cell carcinomas in rats

Xinwei Li, Yoshihisa Fujikura, Yu Hsueh Wang, Tomoo Sawada, Nobuko Tokuda, Rehana Sultana Lovely, Yoshikazu Hayatsu, Tetsuo Fukumoto, Fumihiko Shinozaki

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

F6 tumor cells are derived from a spontaneous rat squamous cell carcinoma (SCC) which originally arose in the facial skin of a DA rat. In this study, FF6 tumor cells were implanted into rat oral mucosa to establish an ex vivo metastatic model. We analyzed the expression of intercellular cell adhesion molecule-1 (ICAM-1) in the implanted primary and metastatic FF6 tumors by immunostaining with a monoclonal antibody (mAb) against ICAM-1. The implanted primary FF6 cells showed strong expression of ICAM-1, whereas the tumor cells of metastatic lesions showed weak or negative expression of ICAM-1. By immunostaining with mAb OX6, a number of MHC class II-positive macrophages were detected in tumor mesenchyme and surrounding the metastatic foci. These results suggested that the local immune reaction in the lymph node influenced the expression of ICAM-1 on tumor cells, and that MHC class II-positive macrophages may play a role in transplanted tumor growth and metastases.

Original languageEnglish
Pages (from-to)371-376
Number of pages6
JournalJournal of Oral Pathology and Medicine
Volume26
Issue number8
DOIs
StatePublished - 1 Jan 1997

Fingerprint

Cell Adhesion Molecules
Intercellular Adhesion Molecule-1
Squamous Cell Carcinoma
Neoplasms
Macrophages
Monoclonal Antibodies
Mouth Mucosa
Mesoderm
Lymph Nodes
Neoplasm Metastasis
Skin
Growth

Keywords

  • FF6
  • Intercellular cell adhesion molecule-1 (ICAM-1)
  • Metastasis
  • Squamous cell carcinoma (SCC)

Cite this

Li, Xinwei ; Fujikura, Yoshihisa ; Wang, Yu Hsueh ; Sawada, Tomoo ; Tokuda, Nobuko ; Lovely, Rehana Sultana ; Hayatsu, Yoshikazu ; Fukumoto, Tetsuo ; Shinozaki, Fumihiko. / Expression of ICAM-1 in implanted primary and metastatic squamous cell carcinomas in rats. In: Journal of Oral Pathology and Medicine. 1997 ; Vol. 26, No. 8. pp. 371-376.
@article{4d86b818da804b37b6537982a4845a12,
title = "Expression of ICAM-1 in implanted primary and metastatic squamous cell carcinomas in rats",
abstract = "F6 tumor cells are derived from a spontaneous rat squamous cell carcinoma (SCC) which originally arose in the facial skin of a DA rat. In this study, FF6 tumor cells were implanted into rat oral mucosa to establish an ex vivo metastatic model. We analyzed the expression of intercellular cell adhesion molecule-1 (ICAM-1) in the implanted primary and metastatic FF6 tumors by immunostaining with a monoclonal antibody (mAb) against ICAM-1. The implanted primary FF6 cells showed strong expression of ICAM-1, whereas the tumor cells of metastatic lesions showed weak or negative expression of ICAM-1. By immunostaining with mAb OX6, a number of MHC class II-positive macrophages were detected in tumor mesenchyme and surrounding the metastatic foci. These results suggested that the local immune reaction in the lymph node influenced the expression of ICAM-1 on tumor cells, and that MHC class II-positive macrophages may play a role in transplanted tumor growth and metastases.",
keywords = "FF6, Intercellular cell adhesion molecule-1 (ICAM-1), Metastasis, Squamous cell carcinoma (SCC)",
author = "Xinwei Li and Yoshihisa Fujikura and Wang, {Yu Hsueh} and Tomoo Sawada and Nobuko Tokuda and Lovely, {Rehana Sultana} and Yoshikazu Hayatsu and Tetsuo Fukumoto and Fumihiko Shinozaki",
year = "1997",
month = "1",
day = "1",
doi = "10.1111/j.1600-0714.1997.tb00233.x",
language = "English",
volume = "26",
pages = "371--376",
journal = "Journal of Oral Pathology and Medicine",
issn = "0904-2512",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "8",

}

Li, X, Fujikura, Y, Wang, YH, Sawada, T, Tokuda, N, Lovely, RS, Hayatsu, Y, Fukumoto, T & Shinozaki, F 1997, 'Expression of ICAM-1 in implanted primary and metastatic squamous cell carcinomas in rats', Journal of Oral Pathology and Medicine, vol. 26, no. 8, pp. 371-376. https://doi.org/10.1111/j.1600-0714.1997.tb00233.x

Expression of ICAM-1 in implanted primary and metastatic squamous cell carcinomas in rats. / Li, Xinwei; Fujikura, Yoshihisa; Wang, Yu Hsueh; Sawada, Tomoo; Tokuda, Nobuko; Lovely, Rehana Sultana; Hayatsu, Yoshikazu; Fukumoto, Tetsuo; Shinozaki, Fumihiko.

In: Journal of Oral Pathology and Medicine, Vol. 26, No. 8, 01.01.1997, p. 371-376.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Expression of ICAM-1 in implanted primary and metastatic squamous cell carcinomas in rats

AU - Li, Xinwei

AU - Fujikura, Yoshihisa

AU - Wang, Yu Hsueh

AU - Sawada, Tomoo

AU - Tokuda, Nobuko

AU - Lovely, Rehana Sultana

AU - Hayatsu, Yoshikazu

AU - Fukumoto, Tetsuo

AU - Shinozaki, Fumihiko

PY - 1997/1/1

Y1 - 1997/1/1

N2 - F6 tumor cells are derived from a spontaneous rat squamous cell carcinoma (SCC) which originally arose in the facial skin of a DA rat. In this study, FF6 tumor cells were implanted into rat oral mucosa to establish an ex vivo metastatic model. We analyzed the expression of intercellular cell adhesion molecule-1 (ICAM-1) in the implanted primary and metastatic FF6 tumors by immunostaining with a monoclonal antibody (mAb) against ICAM-1. The implanted primary FF6 cells showed strong expression of ICAM-1, whereas the tumor cells of metastatic lesions showed weak or negative expression of ICAM-1. By immunostaining with mAb OX6, a number of MHC class II-positive macrophages were detected in tumor mesenchyme and surrounding the metastatic foci. These results suggested that the local immune reaction in the lymph node influenced the expression of ICAM-1 on tumor cells, and that MHC class II-positive macrophages may play a role in transplanted tumor growth and metastases.

AB - F6 tumor cells are derived from a spontaneous rat squamous cell carcinoma (SCC) which originally arose in the facial skin of a DA rat. In this study, FF6 tumor cells were implanted into rat oral mucosa to establish an ex vivo metastatic model. We analyzed the expression of intercellular cell adhesion molecule-1 (ICAM-1) in the implanted primary and metastatic FF6 tumors by immunostaining with a monoclonal antibody (mAb) against ICAM-1. The implanted primary FF6 cells showed strong expression of ICAM-1, whereas the tumor cells of metastatic lesions showed weak or negative expression of ICAM-1. By immunostaining with mAb OX6, a number of MHC class II-positive macrophages were detected in tumor mesenchyme and surrounding the metastatic foci. These results suggested that the local immune reaction in the lymph node influenced the expression of ICAM-1 on tumor cells, and that MHC class II-positive macrophages may play a role in transplanted tumor growth and metastases.

KW - FF6

KW - Intercellular cell adhesion molecule-1 (ICAM-1)

KW - Metastasis

KW - Squamous cell carcinoma (SCC)

UR - http://www.scopus.com/inward/record.url?scp=0031238794&partnerID=8YFLogxK

U2 - 10.1111/j.1600-0714.1997.tb00233.x

DO - 10.1111/j.1600-0714.1997.tb00233.x

M3 - Article

C2 - 9379427

AN - SCOPUS:0031238794

VL - 26

SP - 371

EP - 376

JO - Journal of Oral Pathology and Medicine

JF - Journal of Oral Pathology and Medicine

SN - 0904-2512

IS - 8

ER -