Expression of human chromosomal proteins HMG-14 and HMG-17 in Saccharomyces cerevisiae

Srikantha Thyagarajan, Ravi Dhar, Michael Bustin

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Abstract

The cDNAs coding for human chromosomal proteins HMG-14 and HMG-17 were cloned into yeast expression vector pBM150, under the control of the Gal10 promoter. Northern analysis of transformed yeast cells revealed that both cDNAs were efficiently transcribed. Western analysis indicated that the mRNAs were translated into authentic proteins. Expression of human HMG proteins in yeast cell did not produce detectable phenotypic changes, as measured by the growth rate of the yeast cells under a variety of conditions. The antibiotic resistance of the transfected cells was similar to that of control cells, suggesting that the presence of HMG did not affect the expression of actively transcribed genes. However, examination of the protein profile on two-dimensional polyacrylamide gel electrophoresis revealed differences between control and HMG-transfected Cells.

Original languageEnglish
Pages (from-to)71-75
Number of pages5
JournalExperimental Cell Research
Volume191
Issue number1
DOIs
StatePublished - 1 Jan 1990

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