Expression and characterization of recombinant human lecithin:cholesterol acyltransferase

J. S. Hill, O. Karmin, X. Wang, S. Paranjape, D. Dimitrijevich, A. G. Lacko, P. H. Pritchard

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We have established a baby hamster kidney (BHK) cell line that constitutively expresses significant quantities of human recombinant lecithin:cholesterol acyltransferase (rLCAT). LCAT cDNA was cloned into a mammalian expression vector containing the metallothionein promoter and the dihydrofolate reductase gene. After transfection, the BHK cells were treated with 500 μM methotrexate for 2 weeks to select the successfully transfected cells. Surviving colonies were subcloned and high level secretors were identified by measurement of LCAT activity and mass in the culture medium. The attachment of transfected cells to microcarrier beads enabled the efficient production of large quantities of rLCAT in a serum-free medium. After a single-step chromatography procedure, the rLCAT was purified to homogeneity with yields exceeding 1 mg of rLCAT per 100 ml of culture medium. The molecular weight of rLCAT (≃ 66,000) was identical to that of purified human plasma LCAT on SDS polyacrylamide electrophoresis. The rLCAT was activated by apolipoprotein A-I and had an average specific activity that was similar to purified plasma LCAT. After selective deglycosylation with either neuraminidase or N-glycanase, rLCAT and plasma LCAT had identical molecular weights. The simplification of the production and purification of rLCAT reported here will enable a more in depth analysis of the structure and function of this enzyme.

Original languageEnglish
Pages (from-to)1245-1251
Number of pages7
JournalJournal of Lipid Research
Volume34
Issue number7
StatePublished - 1 Jan 1993

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Phosphatidylcholine-Sterol O-Acyltransferase
Cricetinae
Culture Media
Molecular Weight
Molecular weight
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
Plasma (human)
Kidney
Plasmas
Tetrahydrofolate Dehydrogenase
Metallothionein
Apolipoprotein A-I
Serum-Free Culture Media
Neuraminidase
Chromatography
Electrophoresis
Methotrexate
Purification
Transfection
Complementary DNA

Keywords

  • DNA transfection
  • enzymatic deglycosylation
  • expression
  • recombinant protein

Cite this

Hill, J. S., Karmin, O., Wang, X., Paranjape, S., Dimitrijevich, D., Lacko, A. G., & Pritchard, P. H. (1993). Expression and characterization of recombinant human lecithin:cholesterol acyltransferase. Journal of Lipid Research, 34(7), 1245-1251.
Hill, J. S. ; Karmin, O. ; Wang, X. ; Paranjape, S. ; Dimitrijevich, D. ; Lacko, A. G. ; Pritchard, P. H. / Expression and characterization of recombinant human lecithin:cholesterol acyltransferase. In: Journal of Lipid Research. 1993 ; Vol. 34, No. 7. pp. 1245-1251.
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Hill, JS, Karmin, O, Wang, X, Paranjape, S, Dimitrijevich, D, Lacko, AG & Pritchard, PH 1993, 'Expression and characterization of recombinant human lecithin:cholesterol acyltransferase', Journal of Lipid Research, vol. 34, no. 7, pp. 1245-1251.

Expression and characterization of recombinant human lecithin:cholesterol acyltransferase. / Hill, J. S.; Karmin, O.; Wang, X.; Paranjape, S.; Dimitrijevich, D.; Lacko, A. G.; Pritchard, P. H.

In: Journal of Lipid Research, Vol. 34, No. 7, 01.01.1993, p. 1245-1251.

Research output: Contribution to journalArticle

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T1 - Expression and characterization of recombinant human lecithin:cholesterol acyltransferase

AU - Hill, J. S.

AU - Karmin, O.

AU - Wang, X.

AU - Paranjape, S.

AU - Dimitrijevich, D.

AU - Lacko, A. G.

AU - Pritchard, P. H.

PY - 1993/1/1

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N2 - We have established a baby hamster kidney (BHK) cell line that constitutively expresses significant quantities of human recombinant lecithin:cholesterol acyltransferase (rLCAT). LCAT cDNA was cloned into a mammalian expression vector containing the metallothionein promoter and the dihydrofolate reductase gene. After transfection, the BHK cells were treated with 500 μM methotrexate for 2 weeks to select the successfully transfected cells. Surviving colonies were subcloned and high level secretors were identified by measurement of LCAT activity and mass in the culture medium. The attachment of transfected cells to microcarrier beads enabled the efficient production of large quantities of rLCAT in a serum-free medium. After a single-step chromatography procedure, the rLCAT was purified to homogeneity with yields exceeding 1 mg of rLCAT per 100 ml of culture medium. The molecular weight of rLCAT (≃ 66,000) was identical to that of purified human plasma LCAT on SDS polyacrylamide electrophoresis. The rLCAT was activated by apolipoprotein A-I and had an average specific activity that was similar to purified plasma LCAT. After selective deglycosylation with either neuraminidase or N-glycanase, rLCAT and plasma LCAT had identical molecular weights. The simplification of the production and purification of rLCAT reported here will enable a more in depth analysis of the structure and function of this enzyme.

AB - We have established a baby hamster kidney (BHK) cell line that constitutively expresses significant quantities of human recombinant lecithin:cholesterol acyltransferase (rLCAT). LCAT cDNA was cloned into a mammalian expression vector containing the metallothionein promoter and the dihydrofolate reductase gene. After transfection, the BHK cells were treated with 500 μM methotrexate for 2 weeks to select the successfully transfected cells. Surviving colonies were subcloned and high level secretors were identified by measurement of LCAT activity and mass in the culture medium. The attachment of transfected cells to microcarrier beads enabled the efficient production of large quantities of rLCAT in a serum-free medium. After a single-step chromatography procedure, the rLCAT was purified to homogeneity with yields exceeding 1 mg of rLCAT per 100 ml of culture medium. The molecular weight of rLCAT (≃ 66,000) was identical to that of purified human plasma LCAT on SDS polyacrylamide electrophoresis. The rLCAT was activated by apolipoprotein A-I and had an average specific activity that was similar to purified plasma LCAT. After selective deglycosylation with either neuraminidase or N-glycanase, rLCAT and plasma LCAT had identical molecular weights. The simplification of the production and purification of rLCAT reported here will enable a more in depth analysis of the structure and function of this enzyme.

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Hill JS, Karmin O, Wang X, Paranjape S, Dimitrijevich D, Lacko AG et al. Expression and characterization of recombinant human lecithin:cholesterol acyltransferase. Journal of Lipid Research. 1993 Jan 1;34(7):1245-1251.