Abstract
Previous studies examining whether splitting the DNA extract for replicate amplification versus maximizing the template available for a ‘one-shot’ amplification either examined the benefits of using replicates (without a comparison to a single amplification), or used semi-continuous probabilistic software that ignores peak height information. In this study, we use a fully continuous probabilistic genotyping software to compare the effectiveness of amplifying a single sample compared to splitting the sample and conducting a joint analysis of replicate amplifications. We show that the one-shot approach is marginally better than splitting the DNA extract across a range of contributor numbers and template amounts. Where there is unexpected peak height variability or drop-in within the profile not modelled during interpretation, a replicate approach may be better.
Original language | English |
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Pages (from-to) | 584-595 |
Number of pages | 12 |
Journal | Australian Journal of Forensic Sciences |
Volume | 54 |
Issue number | 5 |
DOIs | |
State | Published - 2022 |
Keywords
- DNA mixture
- Forensic DNA
- mixture interpretation
- probabilistic genotyping
- replicate PCR