Dansyl-Pro-Gln-Arg-NH2, an N-terminally modified tripeptide amide and a putative neuropeptide FF antagonist, was amenable to both positive-ion ESI and APCL. The protonated molecule yielded several fragment ions upon collision-induced dissociation in a quadrupole ion trap instrument for the development of LC/MS/MS assay methods. ESI clearly outperformed APCI in limits of detection, and was the method of choice for coupling with narrow-bore reversed-phase liquid chromatography to assess the pharmacokinetic profile and brain concentration of the neuropeptide FF antagonist in experimental animals. While plasma could be analyzed after rapid sample preparation, brain tissue required cleanup (solid phase extraction) and preconcentration before injection, and the assay was prone to matrix interference. This study indicated a rapid disappearance of dansyl-Pro-Gln-Arg-NH2 from the plasma and the brain, and modest CNS bioavailability after intravenous administration to rats. Copyright (C) 2000 John Wiley and Sons, Ltd.
|Number of pages||7|
|Journal||Rapid Communications in Mass Spectrometry|
|State||Published - 1 Dec 2000|