Exosome-associated hepatitis C virus in cell cultures and patient plasma

Ziqing Liu; Xiugen Zhang; Qigui Yu; Johnny J. He

doi:10.1016/j.bbrc.2014.10.146

Exosome-associated hepatitis C virus in cell cultures and patient plasma

Ziqing Liu, Xiugen Zhang, Qigui Yu, Johnny J. He

Microbiology, Immunology & Genetics

Research output: Contribution to journal › Article › peer-review

56 Scopus citations

Abstract

Hepatitis C virus (HCV) infects its target cells in the form of cell-free viruses and through cell-cell contact. Here we report that HCV is associated with exosomes. Using highly purified exosomes and transmission electron microscopic imaging, we demonstrated that HCV occurred in both exosome-free and exosome-associated forms. Exosome-associated HCV was infectious and resistant to neutralization by an anti-HCV neutralizing antibody. There were more exosome-associated HCV than exosome-free HCV detected in the plasma of HCV-infected patients. These results suggest exosome-associated HCV as an alternative form for HCV infection and transmission.

Original language	English
Pages (from-to)	218-222
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	455
Issue number	3-4
DOIs	https://doi.org/10.1016/j.bbrc.2014.10.146
State	Published - 12 Dec 2014

Keywords

Exosome
HCV
Infection
Neutralizing antibody
Patient plasma
TEM imaging

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AU - He, Johnny J.

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AB - Hepatitis C virus (HCV) infects its target cells in the form of cell-free viruses and through cell-cell contact. Here we report that HCV is associated with exosomes. Using highly purified exosomes and transmission electron microscopic imaging, we demonstrated that HCV occurred in both exosome-free and exosome-associated forms. Exosome-associated HCV was infectious and resistant to neutralization by an anti-HCV neutralizing antibody. There were more exosome-associated HCV than exosome-free HCV detected in the plasma of HCV-infected patients. These results suggest exosome-associated HCV as an alternative form for HCV infection and transmission.

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KW - Infection

KW - Neutralizing antibody

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Exosome-associated hepatitis C virus in cell cultures and patient plasma

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