Exon-level expression profiling of ocular tissues

Alex H. Wagner, V. Nikhil Anand, Wan Heng Wang, Jon E. Chatterton, Duo Sun, Allan R. Shepard, Nasreen Jacobson, Iok Hou Pang, Adam P. DeLuca, Thomas L. Casavant, Todd E. Scheetz, Robert F. Mullins, Terry A. Braun, Abbot F. Clark

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Abstract

The normal gene expression profiles of the tissues in the eye are a valuable resource for considering genes likely to be involved with disease processes. We profiled gene expression in ten ocular tissues from human donor eyes using Affymetrix Human Exon 1.0 ST arrays. Ten different tissues were obtained from six different individuals and RNA was pooled. The tissues included: retina, optic nerve head (ONH), optic nerve (ON), ciliary body (CB), trabecular meshwork (TM), sclera, lens, cornea, choroid/retinal pigment epithelium (RPE) and iris. Expression values were compared with publically available Expressed Sequence Tag (EST) and RNA-sequencing resources. Known tissue-specific genes were examined and they demonstrated correspondence of expression with the representative ocular tissues. The estimated gene and exon level abundances are available online at the Ocular Tissue Database.

Original languageEnglish
Pages (from-to)105-111
Number of pages7
JournalExperimental eye research
Volume111
DOIs
StatePublished - 1 Jun 2013

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Keywords

  • Database
  • Expression profile
  • Eye
  • Gene expression
  • Tissues

Cite this

Wagner, A. H., Anand, V. N., Wang, W. H., Chatterton, J. E., Sun, D., Shepard, A. R., Jacobson, N., Pang, I. H., DeLuca, A. P., Casavant, T. L., Scheetz, T. E., Mullins, R. F., Braun, T. A., & Clark, A. F. (2013). Exon-level expression profiling of ocular tissues. Experimental eye research, 111, 105-111. https://doi.org/10.1016/j.exer.2013.03.004