Evidence for myosin-linked regulation in guinea pig taenia coli muscle

Julian Borejdo, Avraham Oplatka

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The ATPase activity of actomyosin prepared from taenia coli muscle of guinea pig was found to increase upon adding rabbit skeletal heavy meromyosin (HMM) in the absence of Ca2+. SDS-gel electrophoresis of muscle homogenates did not reveal the presence of troponin. Ca2+-regulation in taenia coli muscle thus appears to be myosin-linked. The glycerinated muscles which did not develop any tension in the presence of EGTA contracted after irrigation with rabbit skeletal myosin. Skeletal HMM could also cause tension generation in strips of glycerinated taenia coli in the presence of EGTA. The tension developed by the muscles in the presence of Ca2+ was increased if HMM was added. The HMM-induced tension was associated with a marked increase in ATPase activity both in the presence and in the absence of Ca2+. No HMM-associated tension could be detected when inactivated HMM was employed or when MgATP was substituted with Mg-pyrophosphate or Mg-AMP-PNP. The mechanical effect of HMM probably results from a mechanochemical interaction between the added HMM and muscle actin.

Original languageEnglish
Pages (from-to)177-184
Number of pages8
JournalPflügers Archiv European Journal of Physiology
Volume366
Issue number2-3
DOIs
StatePublished - 1 Jan 1976

Fingerprint

Myosin Subfragments
Myosins
Muscle
Guinea Pigs
Colon
Muscles
Egtazic Acid
Adenosine Triphosphatases
Adenylyl Imidodiphosphate
Rabbits
Actomyosin
Muscle Tonus
Troponin
Electrophoresis
Irrigation
Actins
Adenosine Triphosphate
Gels

Keywords

  • Ca regulation
  • Heavy meromyosin
  • Muscle contraction
  • Smooth muscle

Cite this

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abstract = "The ATPase activity of actomyosin prepared from taenia coli muscle of guinea pig was found to increase upon adding rabbit skeletal heavy meromyosin (HMM) in the absence of Ca2+. SDS-gel electrophoresis of muscle homogenates did not reveal the presence of troponin. Ca2+-regulation in taenia coli muscle thus appears to be myosin-linked. The glycerinated muscles which did not develop any tension in the presence of EGTA contracted after irrigation with rabbit skeletal myosin. Skeletal HMM could also cause tension generation in strips of glycerinated taenia coli in the presence of EGTA. The tension developed by the muscles in the presence of Ca2+ was increased if HMM was added. The HMM-induced tension was associated with a marked increase in ATPase activity both in the presence and in the absence of Ca2+. No HMM-associated tension could be detected when inactivated HMM was employed or when MgATP was substituted with Mg-pyrophosphate or Mg-AMP-PNP. The mechanical effect of HMM probably results from a mechanochemical interaction between the added HMM and muscle actin.",
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Evidence for myosin-linked regulation in guinea pig taenia coli muscle. / Borejdo, Julian; Oplatka, Avraham.

In: Pflügers Archiv European Journal of Physiology, Vol. 366, No. 2-3, 01.01.1976, p. 177-184.

Research output: Contribution to journalArticle

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