Evaluation of the precision ID whole MtDNA genome panel for forensic analyses

Christina Strobl; Mayra Eduardoff; Magdalena M. Bus; Marie Allen; Walther Parson

doi:10.1016/j.fsigen.2018.03.013

Evaluation of the precision ID whole MtDNA genome panel for forensic analyses

Christina Strobl, Mayra Eduardoff, Magdalena M. Bus, Marie Allen, Walther Parson

Research output: Contribution to journal › Article › peer-review

63 Scopus citations

Abstract

Mitochondrial DNA (mtDNA) amplification and Massively Parallel Sequencing (MPS) using an early access version of the Precision ID Whole MtDNA Genome Panel (Thermo Fisher Scientific) and the Ion Personal Genome Machine (PGM) were evaluated using 15 forensically relevant samples. Samples were selected to represent typical forensic specimens for mtDNA analysis including hairs, hair shafts, swabs and ancient solid tissue samples (bones and teeth) that were stored in the freezer for up to several years after having been typed with conventional Sanger-type Sequencing and Capillary Electrophoresis. The MPS haplotypes confirmed the earlier results in all samples and provided additional sequence information that improved discrimination power and haplogroup estimation. The results raised the appetite for further experiments to validate and apply the new technology in forensic practice.

Original language	English
Pages (from-to)	21-25
Number of pages	5
Journal	Forensic Science International: Genetics
Volume	35
DOIs	https://doi.org/10.1016/j.fsigen.2018.03.013
State	Published - Jul 2018

Keywords

Degraded DNA
Forensic genetics
Massively parallel sequencing
Mini-amplicon
Mitochondrial DNA
Mitogenome
Next generation sequencing
Primer tiling

Access to Document

10.1016/j.fsigen.2018.03.013

Cite this

@article{7c31662cbc484413ba99bf31d7d9dae4,

title = "Evaluation of the precision ID whole MtDNA genome panel for forensic analyses",

abstract = "Mitochondrial DNA (mtDNA) amplification and Massively Parallel Sequencing (MPS) using an early access version of the Precision ID Whole MtDNA Genome Panel (Thermo Fisher Scientific) and the Ion Personal Genome Machine (PGM) were evaluated using 15 forensically relevant samples. Samples were selected to represent typical forensic specimens for mtDNA analysis including hairs, hair shafts, swabs and ancient solid tissue samples (bones and teeth) that were stored in the freezer for up to several years after having been typed with conventional Sanger-type Sequencing and Capillary Electrophoresis. The MPS haplotypes confirmed the earlier results in all samples and provided additional sequence information that improved discrimination power and haplogroup estimation. The results raised the appetite for further experiments to validate and apply the new technology in forensic practice.",

keywords = "Degraded DNA, Forensic genetics, Massively parallel sequencing, Mini-amplicon, Mitochondrial DNA, Mitogenome, Next generation sequencing, Primer tiling",

author = "Christina Strobl and Mayra Eduardoff and Bus, {Magdalena M.} and Marie Allen and Walther Parson",

note = "Publisher Copyright: {\textcopyright} 2018 Elsevier B.V.",

year = "2018",

month = jul,

doi = "10.1016/j.fsigen.2018.03.013",

language = "English",

volume = "35",

pages = "21--25",

journal = "Forensic Science International: Genetics",

issn = "1872-4973",

publisher = "Elsevier Ireland Ltd",

}

TY - JOUR

T1 - Evaluation of the precision ID whole MtDNA genome panel for forensic analyses

AU - Strobl, Christina

AU - Eduardoff, Mayra

AU - Bus, Magdalena M.

AU - Allen, Marie

AU - Parson, Walther

PY - 2018/7

Y1 - 2018/7

N2 - Mitochondrial DNA (mtDNA) amplification and Massively Parallel Sequencing (MPS) using an early access version of the Precision ID Whole MtDNA Genome Panel (Thermo Fisher Scientific) and the Ion Personal Genome Machine (PGM) were evaluated using 15 forensically relevant samples. Samples were selected to represent typical forensic specimens for mtDNA analysis including hairs, hair shafts, swabs and ancient solid tissue samples (bones and teeth) that were stored in the freezer for up to several years after having been typed with conventional Sanger-type Sequencing and Capillary Electrophoresis. The MPS haplotypes confirmed the earlier results in all samples and provided additional sequence information that improved discrimination power and haplogroup estimation. The results raised the appetite for further experiments to validate and apply the new technology in forensic practice.

AB - Mitochondrial DNA (mtDNA) amplification and Massively Parallel Sequencing (MPS) using an early access version of the Precision ID Whole MtDNA Genome Panel (Thermo Fisher Scientific) and the Ion Personal Genome Machine (PGM) were evaluated using 15 forensically relevant samples. Samples were selected to represent typical forensic specimens for mtDNA analysis including hairs, hair shafts, swabs and ancient solid tissue samples (bones and teeth) that were stored in the freezer for up to several years after having been typed with conventional Sanger-type Sequencing and Capillary Electrophoresis. The MPS haplotypes confirmed the earlier results in all samples and provided additional sequence information that improved discrimination power and haplogroup estimation. The results raised the appetite for further experiments to validate and apply the new technology in forensic practice.

KW - Degraded DNA

KW - Forensic genetics

KW - Massively parallel sequencing

KW - Mini-amplicon

KW - Mitochondrial DNA

KW - Mitogenome

KW - Next generation sequencing

KW - Primer tiling

UR - http://www.scopus.com/inward/record.url?scp=85044943476&partnerID=8YFLogxK

U2 - 10.1016/j.fsigen.2018.03.013

DO - 10.1016/j.fsigen.2018.03.013

M3 - Article

C2 - 29626805

AN - SCOPUS:85044943476

SN - 1872-4973

VL - 35

SP - 21

EP - 25

JO - Forensic Science International: Genetics

JF - Forensic Science International: Genetics

ER -

Evaluation of the precision ID whole MtDNA genome panel for forensic analyses

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this