TY - JOUR
T1 - Evaluation of instrument response functions for lifetime imaging detectors using quenched Rose Bengal solutions
AU - Szabelski, Mariusz
AU - Luchowski, Rafal
AU - Gryczynski, Zygmunt
AU - Kapusta, Peter
AU - Ortmann, Uwe
AU - Gryczynski, Ignacy
N1 - Funding Information:
This work was supported by Texas Emerging Technologies Fund Grant (CCFT). Mariusz Szabelski and Rafal Luchowski are the recipients of a researcher’s mobility program from the Polish Ministry of Science and Higher Education.
PY - 2009/3/16
Y1 - 2009/3/16
N2 - Instrument response functions (IRF) in time-domain fluorescence are usually recorded as reflected or scattered excitation light, which is at shorter wavelengths than the observed fluorescence emission. However, its often more appropriate to measure the IRF in the emission spectral region. In this work we show that Rose Bengal water solutions quenched by potassium iodide can be used to measure instrument response functions of single photon detectors in the orange-red wavelength region. We used the quenched RB emission as a reference in time-domain measurements with common detectors and we got practically the same results as with scattering.
AB - Instrument response functions (IRF) in time-domain fluorescence are usually recorded as reflected or scattered excitation light, which is at shorter wavelengths than the observed fluorescence emission. However, its often more appropriate to measure the IRF in the emission spectral region. In this work we show that Rose Bengal water solutions quenched by potassium iodide can be used to measure instrument response functions of single photon detectors in the orange-red wavelength region. We used the quenched RB emission as a reference in time-domain measurements with common detectors and we got practically the same results as with scattering.
UR - http://www.scopus.com/inward/record.url?scp=61649083774&partnerID=8YFLogxK
U2 - 10.1016/j.cplett.2009.02.001
DO - 10.1016/j.cplett.2009.02.001
M3 - Article
AN - SCOPUS:61649083774
SN - 0009-2614
VL - 471
SP - 153
EP - 159
JO - Chemical Physics Letters
JF - Chemical Physics Letters
IS - 1-3
ER -