Evaluation of InnoTyper® 21 in a sample of Rio de Janeiro population as an alternative forensic panel

R. S. Moura-Neto, I. C.T. Mello, R. Silva, A. P.C. Maette, C. G. Bottino, August Eric Woerner, J. King, F. Wendt, Bruce Budowle

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The use of bi-allelic markers such as retrotransposable element insertion polymorphisms or Innuls (for insertion/null) can overcome some limitations of short tandem repeat (STR) loci in typing forensic biological evidence. This study investigated the efficiency of the InnoTyper® 21 Innul markers in an urban admixed population sample in Rio de Janeiro (n = 40) and one highly compromised sample collected as evidence by the Rio de Janeiro police. No significant departures from Hardy-Weinberg equilibrium were detected after the Bonferroni correction (α′ ≈ 0.05/20, p < 0.0025), and no significant linkage disequilibrium was observed between markers. Assuming loci independence, the cumulative random match probability (RMP) was 2.3 × 10−8. A lower mean Fis value was obtained for this sample population compared with those of three North American populations (African-American, Southwest Hispanic, US Caucasian). Principal component analysis with the three North American populations and one from 21 East Asian population showed that African Americans segregated as an independent group while US Caucasian, Southwest Hispanic, East Asian, and Rio de Janeiro populations are in a single large heterogeneous group. Also, a full Innuls profile was produced from an evidence sample, despite the DNA being highly degraded. In conclusion, this system is a useful complement to standard STR kits.

Original languageEnglish
Pages (from-to)149-151
Number of pages3
JournalInternational journal of legal medicine
Volume132
Issue number1
DOIs
StatePublished - 1 Jan 2018

Fingerprint

Southwestern United States
Population
Hispanic Americans
African Americans
Microsatellite Repeats
Urban Population
Linkage Disequilibrium
Police
Principal Component Analysis
DNA

Keywords

  • Brazil
  • Forensic genetics
  • Innuls
  • Rio de Janeiro
  • Transposable element markers

Cite this

Moura-Neto, R. S. ; Mello, I. C.T. ; Silva, R. ; Maette, A. P.C. ; Bottino, C. G. ; Woerner, August Eric ; King, J. ; Wendt, F. ; Budowle, Bruce. / Evaluation of InnoTyper® 21 in a sample of Rio de Janeiro population as an alternative forensic panel. In: International journal of legal medicine. 2018 ; Vol. 132, No. 1. pp. 149-151.
@article{f185e12c6fe044ba957f31f465ffe7bc,
title = "Evaluation of InnoTyper{\circledR} 21 in a sample of Rio de Janeiro population as an alternative forensic panel",
abstract = "The use of bi-allelic markers such as retrotransposable element insertion polymorphisms or Innuls (for insertion/null) can overcome some limitations of short tandem repeat (STR) loci in typing forensic biological evidence. This study investigated the efficiency of the InnoTyper{\circledR} 21 Innul markers in an urban admixed population sample in Rio de Janeiro (n = 40) and one highly compromised sample collected as evidence by the Rio de Janeiro police. No significant departures from Hardy-Weinberg equilibrium were detected after the Bonferroni correction (α′ ≈ 0.05/20, p < 0.0025), and no significant linkage disequilibrium was observed between markers. Assuming loci independence, the cumulative random match probability (RMP) was 2.3 × 10−8. A lower mean Fis value was obtained for this sample population compared with those of three North American populations (African-American, Southwest Hispanic, US Caucasian). Principal component analysis with the three North American populations and one from 21 East Asian population showed that African Americans segregated as an independent group while US Caucasian, Southwest Hispanic, East Asian, and Rio de Janeiro populations are in a single large heterogeneous group. Also, a full Innuls profile was produced from an evidence sample, despite the DNA being highly degraded. In conclusion, this system is a useful complement to standard STR kits.",
keywords = "Brazil, Forensic genetics, Innuls, Rio de Janeiro, Transposable element markers",
author = "Moura-Neto, {R. S.} and Mello, {I. C.T.} and R. Silva and Maette, {A. P.C.} and Bottino, {C. G.} and Woerner, {August Eric} and J. King and F. Wendt and Bruce Budowle",
year = "2018",
month = "1",
day = "1",
doi = "10.1007/s00414-017-1642-2",
language = "English",
volume = "132",
pages = "149--151",
journal = "International Journal of Legal Medicine",
issn = "0937-9827",
publisher = "Springer Verlag",
number = "1",

}

Evaluation of InnoTyper® 21 in a sample of Rio de Janeiro population as an alternative forensic panel. / Moura-Neto, R. S.; Mello, I. C.T.; Silva, R.; Maette, A. P.C.; Bottino, C. G.; Woerner, August Eric; King, J.; Wendt, F.; Budowle, Bruce.

In: International journal of legal medicine, Vol. 132, No. 1, 01.01.2018, p. 149-151.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Evaluation of InnoTyper® 21 in a sample of Rio de Janeiro population as an alternative forensic panel

AU - Moura-Neto, R. S.

AU - Mello, I. C.T.

AU - Silva, R.

AU - Maette, A. P.C.

AU - Bottino, C. G.

AU - Woerner, August Eric

AU - King, J.

AU - Wendt, F.

AU - Budowle, Bruce

PY - 2018/1/1

Y1 - 2018/1/1

N2 - The use of bi-allelic markers such as retrotransposable element insertion polymorphisms or Innuls (for insertion/null) can overcome some limitations of short tandem repeat (STR) loci in typing forensic biological evidence. This study investigated the efficiency of the InnoTyper® 21 Innul markers in an urban admixed population sample in Rio de Janeiro (n = 40) and one highly compromised sample collected as evidence by the Rio de Janeiro police. No significant departures from Hardy-Weinberg equilibrium were detected after the Bonferroni correction (α′ ≈ 0.05/20, p < 0.0025), and no significant linkage disequilibrium was observed between markers. Assuming loci independence, the cumulative random match probability (RMP) was 2.3 × 10−8. A lower mean Fis value was obtained for this sample population compared with those of three North American populations (African-American, Southwest Hispanic, US Caucasian). Principal component analysis with the three North American populations and one from 21 East Asian population showed that African Americans segregated as an independent group while US Caucasian, Southwest Hispanic, East Asian, and Rio de Janeiro populations are in a single large heterogeneous group. Also, a full Innuls profile was produced from an evidence sample, despite the DNA being highly degraded. In conclusion, this system is a useful complement to standard STR kits.

AB - The use of bi-allelic markers such as retrotransposable element insertion polymorphisms or Innuls (for insertion/null) can overcome some limitations of short tandem repeat (STR) loci in typing forensic biological evidence. This study investigated the efficiency of the InnoTyper® 21 Innul markers in an urban admixed population sample in Rio de Janeiro (n = 40) and one highly compromised sample collected as evidence by the Rio de Janeiro police. No significant departures from Hardy-Weinberg equilibrium were detected after the Bonferroni correction (α′ ≈ 0.05/20, p < 0.0025), and no significant linkage disequilibrium was observed between markers. Assuming loci independence, the cumulative random match probability (RMP) was 2.3 × 10−8. A lower mean Fis value was obtained for this sample population compared with those of three North American populations (African-American, Southwest Hispanic, US Caucasian). Principal component analysis with the three North American populations and one from 21 East Asian population showed that African Americans segregated as an independent group while US Caucasian, Southwest Hispanic, East Asian, and Rio de Janeiro populations are in a single large heterogeneous group. Also, a full Innuls profile was produced from an evidence sample, despite the DNA being highly degraded. In conclusion, this system is a useful complement to standard STR kits.

KW - Brazil

KW - Forensic genetics

KW - Innuls

KW - Rio de Janeiro

KW - Transposable element markers

UR - http://www.scopus.com/inward/record.url?scp=85026894708&partnerID=8YFLogxK

U2 - 10.1007/s00414-017-1642-2

DO - 10.1007/s00414-017-1642-2

M3 - Article

C2 - 28748403

AN - SCOPUS:85026894708

VL - 132

SP - 149

EP - 151

JO - International Journal of Legal Medicine

JF - International Journal of Legal Medicine

SN - 0937-9827

IS - 1

ER -