Ethanol withdrawal reduces cerebellar parvalbumin expression in a manner reversed by estrogens

Mridula Rewal; Yi Wen; James W. Simpkins; Marianna E. Jung

doi:10.1016/j.neulet.2004.11.067

Ethanol withdrawal reduces cerebellar parvalbumin expression in a manner reversed by estrogens

Mridula Rewal, Yi Wen, James W. Simpkins, Marianna E. Jung

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Abstract

Parvalbumin (PA) is a calcium-binding protein that has been implicated in neuroprotection. We examined whether the stimulus effect of ethanol withdrawal (EW) alters the expression of PA in a manner that is prevented by 17β-estradiol (E2). Ovariectomized rats implanted with E2 (EW/E2) or oil (EW/Oil) pellets received chronic ethanol (7.5%, w/v, 5 weeks) or control dextrin diets (Dex/Oil). At 24 h of EW, rats were tested for overt EW signs, and the cerebellum was prepared for immunoblotting and immunohistological assessment for PA. The EW/Oil group showed a higher EW sign score, a lower PA expression, and fewer PA-positive Purkinje neurons than the dextrin control group. In the EW/E2 group, EW sign scores, PA expression, and PA-positive Purkinje neurons were not significantly different from those in the control dextrin group. These data suggest that E2 treatment protects against the PA-suppression associated with EW toxicity.

Original language	English
Pages (from-to)	44-48
Number of pages	5
Journal	Neuroscience letters
Volume	377
Issue number	1
DOIs	https://doi.org/10.1016/j.neulet.2004.11.067
State	Published - 22 Mar 2005

Keywords

Estrogens
Ethanol withdrawal
GABA
Parvalbumin

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10.1016/j.neulet.2004.11.067

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title = "Ethanol withdrawal reduces cerebellar parvalbumin expression in a manner reversed by estrogens",

abstract = "Parvalbumin (PA) is a calcium-binding protein that has been implicated in neuroprotection. We examined whether the stimulus effect of ethanol withdrawal (EW) alters the expression of PA in a manner that is prevented by 17β-estradiol (E2). Ovariectomized rats implanted with E2 (EW/E2) or oil (EW/Oil) pellets received chronic ethanol (7.5%, w/v, 5 weeks) or control dextrin diets (Dex/Oil). At 24 h of EW, rats were tested for overt EW signs, and the cerebellum was prepared for immunoblotting and immunohistological assessment for PA. The EW/Oil group showed a higher EW sign score, a lower PA expression, and fewer PA-positive Purkinje neurons than the dextrin control group. In the EW/E2 group, EW sign scores, PA expression, and PA-positive Purkinje neurons were not significantly different from those in the control dextrin group. These data suggest that E2 treatment protects against the PA-suppression associated with EW toxicity.",

keywords = "Estrogens, Ethanol withdrawal, GABA, Parvalbumin",

author = "Mridula Rewal and Yi Wen and Simpkins, {James W.} and Jung, {Marianna E.}",

note = "Funding Information: This work was supported by NIH/NIAAA013864. ",

year = "2005",

month = mar,

day = "22",

doi = "10.1016/j.neulet.2004.11.067",

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journal = "Neuroscience Letters",

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T1 - Ethanol withdrawal reduces cerebellar parvalbumin expression in a manner reversed by estrogens

AU - Rewal, Mridula

AU - Wen, Yi

AU - Simpkins, James W.

AU - Jung, Marianna E.

N1 - Funding Information: This work was supported by NIH/NIAAA013864.

PY - 2005/3/22

Y1 - 2005/3/22

N2 - Parvalbumin (PA) is a calcium-binding protein that has been implicated in neuroprotection. We examined whether the stimulus effect of ethanol withdrawal (EW) alters the expression of PA in a manner that is prevented by 17β-estradiol (E2). Ovariectomized rats implanted with E2 (EW/E2) or oil (EW/Oil) pellets received chronic ethanol (7.5%, w/v, 5 weeks) or control dextrin diets (Dex/Oil). At 24 h of EW, rats were tested for overt EW signs, and the cerebellum was prepared for immunoblotting and immunohistological assessment for PA. The EW/Oil group showed a higher EW sign score, a lower PA expression, and fewer PA-positive Purkinje neurons than the dextrin control group. In the EW/E2 group, EW sign scores, PA expression, and PA-positive Purkinje neurons were not significantly different from those in the control dextrin group. These data suggest that E2 treatment protects against the PA-suppression associated with EW toxicity.

AB - Parvalbumin (PA) is a calcium-binding protein that has been implicated in neuroprotection. We examined whether the stimulus effect of ethanol withdrawal (EW) alters the expression of PA in a manner that is prevented by 17β-estradiol (E2). Ovariectomized rats implanted with E2 (EW/E2) or oil (EW/Oil) pellets received chronic ethanol (7.5%, w/v, 5 weeks) or control dextrin diets (Dex/Oil). At 24 h of EW, rats were tested for overt EW signs, and the cerebellum was prepared for immunoblotting and immunohistological assessment for PA. The EW/Oil group showed a higher EW sign score, a lower PA expression, and fewer PA-positive Purkinje neurons than the dextrin control group. In the EW/E2 group, EW sign scores, PA expression, and PA-positive Purkinje neurons were not significantly different from those in the control dextrin group. These data suggest that E2 treatment protects against the PA-suppression associated with EW toxicity.

KW - Estrogens

KW - Ethanol withdrawal

KW - GABA

KW - Parvalbumin

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DO - 10.1016/j.neulet.2004.11.067

M3 - Article

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SP - 44

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JO - Neuroscience Letters

JF - Neuroscience Letters

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Ethanol withdrawal reduces cerebellar parvalbumin expression in a manner reversed by estrogens

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Keywords

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