Ethanol withdrawal posttranslationally decreases the activity of cytochrome c oxidase in an estrogen reversible manner

Eunsun Jung, Rajnee Agarwal, James W. Simpkins

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Cytochrome c oxidase (COX) is a key mitochondrial enzyme that catalyzes electron transfer at the terminal stage of respiratory chain and is composed of multisubunits. We hypothesize that ethanol withdrawal (EW) impairs the activity of COX and estrogen deprivation exacerbates this problem. Five-month-old ovariectomized rats with or without 17β-estradiol (E2) replacement received a control dextrin or a liquid ethanol diet (6.5%, 5 weeks). They were then sacrificed either during ethanol exposure or at 24 h of EW (EW group). Mitochondria of the cerebellum and cortex were processed to measure the activities of total COX, COX subunit I, and IV. The effects of EW and E2 on the protein levels of these subunits were also assessed using an immunoblotting method. As compared to the control dextrin and ethanol exposure, EW decreased the activities of total COX, COX I, and COX IV. E2 treatment prevented the effects of EW on the activities of total COX and COX IV but not COX I. Neither EW nor E2 altered the protein levels of the subunits. These findings suggest that a counteracting relationship exists between the effects of EW and E2 on the activity of COX in a subunit specific manner.

Original languageEnglish
Pages (from-to)160-164
Number of pages5
JournalNeuroscience Letters
Volume416
Issue number2
DOIs
StatePublished - 12 Apr 2007

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Electron Transport Complex IV
Estrogens
Ethanol
Oxidoreductases
Protein Subunits
Electron Transport
Immunoblotting
Cerebellum
Estradiol
Mitochondria
Electrons
Diet

Keywords

  • 17β-Estradiol
  • Cytochrome c oxidase
  • Ethanol withdrawal
  • Mitochondria

Cite this

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abstract = "Cytochrome c oxidase (COX) is a key mitochondrial enzyme that catalyzes electron transfer at the terminal stage of respiratory chain and is composed of multisubunits. We hypothesize that ethanol withdrawal (EW) impairs the activity of COX and estrogen deprivation exacerbates this problem. Five-month-old ovariectomized rats with or without 17β-estradiol (E2) replacement received a control dextrin or a liquid ethanol diet (6.5{\%}, 5 weeks). They were then sacrificed either during ethanol exposure or at 24 h of EW (EW group). Mitochondria of the cerebellum and cortex were processed to measure the activities of total COX, COX subunit I, and IV. The effects of EW and E2 on the protein levels of these subunits were also assessed using an immunoblotting method. As compared to the control dextrin and ethanol exposure, EW decreased the activities of total COX, COX I, and COX IV. E2 treatment prevented the effects of EW on the activities of total COX and COX IV but not COX I. Neither EW nor E2 altered the protein levels of the subunits. These findings suggest that a counteracting relationship exists between the effects of EW and E2 on the activity of COX in a subunit specific manner.",
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Ethanol withdrawal posttranslationally decreases the activity of cytochrome c oxidase in an estrogen reversible manner. / Jung, Eunsun; Agarwal, Rajnee; Simpkins, James W.

In: Neuroscience Letters, Vol. 416, No. 2, 12.04.2007, p. 160-164.

Research output: Contribution to journalArticle

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