Ethanol and inositol 1,4,5-trisphosphate mobilize calcium from rat brain microsomes

Tina K. Machu, John J. Woodward, Steven W. Leslie

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

The effects of ethanol on ATP-dependent Ca2+ uptake and inositol 1,4,5 trisphosphate (Ins-P3)-induced Ca2+ release were assessed in rat cerebellar and cortical microsomes. Ins-P3, 0.2-10 μM, released 7-14% of ATP loaded microsomal Ca2+ stores after a 15-sec exposure. Ethanol, 250-750 mM, added after ATP-dependent loading, significantly released microsomal Ca2+ stores, and the amount released was additive to that seen with Ins-P3 alone. The presence of ethanol, 250-750 mM, during the ATP-dependent loading period, resulted in decreased Ca2+ uptake that correlated with decreases in Ins-P3-induced Ca2+ release. Chronic ethanol treatment failed to produce any alterations in ethanol's ability to promote Ca2+ release or to inhibit ATP-dependent Ca2+ uptake. Furthermore, Ins.-P3-induced Ca2+ release was not altered by chronic ethanol treatment. These results suggest that Ins-P3 sensitive Ca2+ stores are resistant to pharmacologically relevant concentrations of ethanol, and do not appear to be involved in the chronic cellular effects of ethanol.

Original languageEnglish
Pages (from-to)431-436
Number of pages6
JournalAlcohol
Volume6
Issue number6
DOIs
StatePublished - 1 Jan 1989

Keywords

  • Calcium
  • Ethanol
  • Inositol 1,4,5-trisphosphate
  • Microsomes
  • Polyphosphoinositides

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