Establishment of stable HeLa cell lines expressing enzymatically active hepatitis C virus RNA polymerase

L. B. Kong, L. B. Ye, L. Ye, Khalid A. Timani, Y. Zheng, Q. J. Liao, B. Z. Li, B. Gao

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)

Abstract

The hepatitis C virus RNA polymerase (NS5B) is strictly required for viral replication and thus represents an attractive target for antiviral drug development. In this study, stable HeLa cell lines with an integrated NS5B gene were selected by G418 and then confirmed by genome PCR. Subsequently, transcription and expression of the integrated NS5B genes were demonstrated by RT-PCR and Western blot analysis. Further analysis demonstrated enzymatic activity of the expressed NS5B polymerase. The stable HeLa cell lines should be useful for the identification of NS5B inhibitors and for studying the mechanisms of HCV replication.

Original languageEnglish
Pages (from-to)361-367
Number of pages7
JournalArchives of Virology
Volume151
Issue number2
DOIs
StatePublished - 1 Feb 2006

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DNA-Directed RNA Polymerases
HeLa Cells
Hepacivirus
Cell Line
Polymerase Chain Reaction
Genes
Antiviral Agents
Western Blotting
Genome
antibiotic G 418

Cite this

Kong, L. B. ; Ye, L. B. ; Ye, L. ; Timani, Khalid A. ; Zheng, Y. ; Liao, Q. J. ; Li, B. Z. ; Gao, B. / Establishment of stable HeLa cell lines expressing enzymatically active hepatitis C virus RNA polymerase. In: Archives of Virology. 2006 ; Vol. 151, No. 2. pp. 361-367.
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Establishment of stable HeLa cell lines expressing enzymatically active hepatitis C virus RNA polymerase. / Kong, L. B.; Ye, L. B.; Ye, L.; Timani, Khalid A.; Zheng, Y.; Liao, Q. J.; Li, B. Z.; Gao, B.

In: Archives of Virology, Vol. 151, No. 2, 01.02.2006, p. 361-367.

Research output: Contribution to journalArticleResearchpeer-review

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