Establishment of stable HeLa cell lines expressing enzymatically active hepatitis C virus RNA polymerase

L. B. Kong, L. B. Ye, L. Ye, K. A. Timani, Y. Zheng, Q. J. Liao, B. Z. Li, B. Gao

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The hepatitis C virus RNA polymerase (NS5B) is strictly required for viral replication and thus represents an attractive target for antiviral drug development. In this study, stable HeLa cell lines with an integrated NS5B gene were selected by G418 and then confirmed by genome PCR. Subsequently, transcription and expression of the integrated NS5B genes were demonstrated by RT-PCR and Western blot analysis. Further analysis demonstrated enzymatic activity of the expressed NS5B polymerase. The stable HeLa cell lines should be useful for the identification of NS5B inhibitors and for studying the mechanisms of HCV replication.

Original languageEnglish
Pages (from-to)361-367
Number of pages7
JournalArchives of Virology
Volume151
Issue number2
DOIs
StatePublished - 1 Feb 2006

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    Kong, L. B., Ye, L. B., Ye, L., Timani, K. A., Zheng, Y., Liao, Q. J., Li, B. Z., & Gao, B. (2006). Establishment of stable HeLa cell lines expressing enzymatically active hepatitis C virus RNA polymerase. Archives of Virology, 151(2), 361-367. https://doi.org/10.1007/s00705-005-0656-0