TY - CHAP
T1 - Establishing the adipose stem cell identity
T2 - Characterization assays and functional properties
AU - Harrison, Mark A.A.
AU - Al-Ghadban, Sara I.
AU - O’Donnell, Benjamen T.
AU - Mohiuddin, Omair A.
AU - Wise, Rachel M.
AU - Sullivan, Brianne N.
AU - Bunnell, Bruce A.
N1 - Publisher Copyright:
© 2022 Elsevier Inc.
PY - 2021/1/1
Y1 - 2021/1/1
N2 - Due to their significant therapeutic potential, adipose stem cells (ASCs) have become the focus of intense of preclinical and clinical research. Characterization of ASCs that is both comprehensive and well standardized is essential for the successful progression of the regenerative medicine field. This chapter provides a detailed outline of the in vitro assays commonly employed for the characterization of ASCs, including flow cytometric assessments of surface markers, self-renewal, and multipotent differentiation. An in-depth examination of adipogenic, osteogenic, and chondrogenic differentiation is covered, including signaling pathways, methodology, and confirmation assays. Furthermore, ASCs possess significant immunomodulatory abilities that make them ideal therapeutic tools. This chapter defines the influence of ASCs on immune cell populations in vitro, including paracrine and cell contact-dependent mechanisms, and summarizes the assays used to quantify this immunoregulation. Research on ASCs is a rapidly evolving field. Rigorous characterization standards, as recommended by the International Federation of Adipose Therapeutics and Sciences (IFATS) and others, must be adopted and maintained by researchers to promote high-impact research, reproducibility, effective collaborations, and acceleration of scientific discoveries in regenerative medicine.
AB - Due to their significant therapeutic potential, adipose stem cells (ASCs) have become the focus of intense of preclinical and clinical research. Characterization of ASCs that is both comprehensive and well standardized is essential for the successful progression of the regenerative medicine field. This chapter provides a detailed outline of the in vitro assays commonly employed for the characterization of ASCs, including flow cytometric assessments of surface markers, self-renewal, and multipotent differentiation. An in-depth examination of adipogenic, osteogenic, and chondrogenic differentiation is covered, including signaling pathways, methodology, and confirmation assays. Furthermore, ASCs possess significant immunomodulatory abilities that make them ideal therapeutic tools. This chapter defines the influence of ASCs on immune cell populations in vitro, including paracrine and cell contact-dependent mechanisms, and summarizes the assays used to quantify this immunoregulation. Research on ASCs is a rapidly evolving field. Rigorous characterization standards, as recommended by the International Federation of Adipose Therapeutics and Sciences (IFATS) and others, must be adopted and maintained by researchers to promote high-impact research, reproducibility, effective collaborations, and acceleration of scientific discoveries in regenerative medicine.
KW - Adipogenesis
KW - Adipose stem cells
KW - Characterization
KW - Chondrogenesis
KW - Differentiation
KW - Immunomodulation
KW - In Vitro
KW - Osteogenesis
UR - http://www.scopus.com/inward/record.url?scp=85123780600&partnerID=8YFLogxK
U2 - 10.1016/B978-0-12-819376-1.00002-0
DO - 10.1016/B978-0-12-819376-1.00002-0
M3 - Chapter
AN - SCOPUS:85123780600
SP - 23
EP - 56
BT - Scientific Principles of Adipose Stem Cells
PB - Elsevier
ER -