Enzyme linked immunoassay (ELISA) for lecithin:cholesterol acyltransferase (LCAT)

Karen R. Murray, Maya P. Nair, Walter J. McConathy, Andras G. Lacko

Research output: Contribution to journalArticle

Abstract

Several immunoassay models, including sandwich ELISA, solid phase ELISA and sink immunoassay and antibody combinations were investigated to develop an ELISA assay for LCAT with an appropriate linear range and sensitivity. Solid phase immunoassays were found to be most suitable for measuring LCAT from cell culture medium and in partially purified preparations. The immunoassays were analyzed for matrix interference, recovery studies, intra- run precision and inter-run precision. These studies have identified a reliable method for measuring LCAT in purified preparations and cell culture media, and provide the foundation for further development of immunoassays for clinical application.

Original languageEnglish
Pages (from-to)1553-1564
Number of pages12
JournalAnalytical Letters
Volume32
Issue number8
DOIs
StatePublished - 1 Jan 1999

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Phosphatidylcholine-Sterol O-Acyltransferase
Lecithin
Cholesterol
Immunoenzyme Techniques
Immunoassay
Enzymes
Cell culture
Culture Media
Antibodies
Cell Culture Techniques
Assays
Recovery

Keywords

  • Acyltransferase
  • ELISA
  • Immunoassay
  • LCAT
  • Lecithin

Cite this

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Enzyme linked immunoassay (ELISA) for lecithin:cholesterol acyltransferase (LCAT). / Murray, Karen R.; Nair, Maya P.; McConathy, Walter J.; Lacko, Andras G.

In: Analytical Letters, Vol. 32, No. 8, 01.01.1999, p. 1553-1564.

Research output: Contribution to journalArticle

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