Several immunoassay models, including sandwich ELISA, solid phase ELISA and sink immunoassay and antibody combinations were investigated to develop an ELISA assay for LCAT with an appropriate linear range and sensitivity. Solid phase immunoassays were found to be most suitable for measuring LCAT from cell culture medium and in partially purified preparations. The immunoassays were analyzed for matrix interference, recovery studies, intra- run precision and inter-run precision. These studies have identified a reliable method for measuring LCAT in purified preparations and cell culture media, and provide the foundation for further development of immunoassays for clinical application.
|Number of pages||12|
|State||Published - 1999|