Enzyme linked immunoassay (ELISA) for lecithin:cholesterol acyltransferase (LCAT)

Karen R. Murray; Maya P. Nair; Walter J. McConathy; Andras G. Lacko

doi:10.1080/00032719908542915

Enzyme linked immunoassay (ELISA) for lecithin:cholesterol acyltransferase (LCAT)

Karen R. Murray, Maya P. Nair, Walter J. McConathy, Andras G. Lacko

Research output: Contribution to journal › Article › peer-review

Abstract

Several immunoassay models, including sandwich ELISA, solid phase ELISA and sink immunoassay and antibody combinations were investigated to develop an ELISA assay for LCAT with an appropriate linear range and sensitivity. Solid phase immunoassays were found to be most suitable for measuring LCAT from cell culture medium and in partially purified preparations. The immunoassays were analyzed for matrix interference, recovery studies, intra- run precision and inter-run precision. These studies have identified a reliable method for measuring LCAT in purified preparations and cell culture media, and provide the foundation for further development of immunoassays for clinical application.

Original language	English
Pages (from-to)	1553-1564
Number of pages	12
Journal	Analytical Letters
Volume	32
Issue number	8
DOIs	https://doi.org/10.1080/00032719908542915
State	Published - 1999

Keywords

Acyltransferase
ELISA
Immunoassay
LCAT
Lecithin

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10.1080/00032719908542915

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title = "Enzyme linked immunoassay (ELISA) for lecithin:cholesterol acyltransferase (LCAT)",

abstract = "Several immunoassay models, including sandwich ELISA, solid phase ELISA and sink immunoassay and antibody combinations were investigated to develop an ELISA assay for LCAT with an appropriate linear range and sensitivity. Solid phase immunoassays were found to be most suitable for measuring LCAT from cell culture medium and in partially purified preparations. The immunoassays were analyzed for matrix interference, recovery studies, intra- run precision and inter-run precision. These studies have identified a reliable method for measuring LCAT in purified preparations and cell culture media, and provide the foundation for further development of immunoassays for clinical application.",

keywords = "Acyltransferase, ELISA, Immunoassay, LCAT, Lecithin",

author = "Murray, {Karen R.} and Nair, {Maya P.} and McConathy, {Walter J.} and Lacko, {Andras G.}",

note = "Funding Information: We thank Dr. P. Haydn Pritchard for making the recombinant.^ BHK and McArdle cells available for these studies. We are grateful to Drs. Clarence Morris and Richard Triplett of Data Medical Associates for consultation and data evaluations throughout these studies. Erik Swansinger provided excellent technical assistance. This research was supported by grants from the Texas Advanced Research Program (009768-003) and the Texas Advanced Technology Program (009768-012).",

year = "1999",

doi = "10.1080/00032719908542915",

language = "English",

volume = "32",

pages = "1553--1564",

journal = "Analytical Letters",

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T1 - Enzyme linked immunoassay (ELISA) for lecithin:cholesterol acyltransferase (LCAT)

AU - Murray, Karen R.

AU - Nair, Maya P.

AU - McConathy, Walter J.

AU - Lacko, Andras G.

N1 - Funding Information: We thank Dr. P. Haydn Pritchard for making the recombinant.^ BHK and McArdle cells available for these studies. We are grateful to Drs. Clarence Morris and Richard Triplett of Data Medical Associates for consultation and data evaluations throughout these studies. Erik Swansinger provided excellent technical assistance. This research was supported by grants from the Texas Advanced Research Program (009768-003) and the Texas Advanced Technology Program (009768-012).

PY - 1999

Y1 - 1999

N2 - Several immunoassay models, including sandwich ELISA, solid phase ELISA and sink immunoassay and antibody combinations were investigated to develop an ELISA assay for LCAT with an appropriate linear range and sensitivity. Solid phase immunoassays were found to be most suitable for measuring LCAT from cell culture medium and in partially purified preparations. The immunoassays were analyzed for matrix interference, recovery studies, intra- run precision and inter-run precision. These studies have identified a reliable method for measuring LCAT in purified preparations and cell culture media, and provide the foundation for further development of immunoassays for clinical application.

AB - Several immunoassay models, including sandwich ELISA, solid phase ELISA and sink immunoassay and antibody combinations were investigated to develop an ELISA assay for LCAT with an appropriate linear range and sensitivity. Solid phase immunoassays were found to be most suitable for measuring LCAT from cell culture medium and in partially purified preparations. The immunoassays were analyzed for matrix interference, recovery studies, intra- run precision and inter-run precision. These studies have identified a reliable method for measuring LCAT in purified preparations and cell culture media, and provide the foundation for further development of immunoassays for clinical application.

KW - Acyltransferase

KW - ELISA

KW - Immunoassay

KW - LCAT

KW - Lecithin

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U2 - 10.1080/00032719908542915

DO - 10.1080/00032719908542915

M3 - Article

AN - SCOPUS:0033064581

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VL - 32

SP - 1553

EP - 1564

JO - Analytical Letters

JF - Analytical Letters

IS - 8

ER -

Enzyme linked immunoassay (ELISA) for lecithin:cholesterol acyltransferase (LCAT)

Abstract

Keywords

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