Enhancement of single-molecule fluorescence signals by colloidal silver nanoparticles in studies of protein translation

Shashank Bharill, Chunlai Chen, Benjamin Stevens, Jaskiran Kaur, Zeev Smilansky, Wlodek Mandecki, Ignacy Gryczynski, Zygmunt Gryczynski, Barry S. Cooperman, Yale E. Goldman

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Metal-enhanced fluorescence (MEF) increased total photon emission of Cy3- and Cy5-labeled ribosomal initiation complexes near 50 nm silver particles 4- and 5.5-fold, respectively. Fluorescence intensity fluctuations above shot noise, at 0.1-5 Hz, were greater on silver particles. Overall signal-to-noise ratio was similar or slightly improved near the particles. Proximity to silver particles did not compromise ribosome function, as measured by codon-dependent binding of fluorescent tRNA, dynamics of fluorescence resonance energy transfer between adjacent tRNAs in the ribosome, and tRNA translocation induced by elongation factor G.

Original languageEnglish
Pages (from-to)399-407
Number of pages9
JournalACS Nano
Volume5
Issue number1
DOIs
StatePublished - 25 Jan 2011

Keywords

  • Metal-enhanced fluorescence
  • Protein translation
  • Ribosome
  • Silver colloids
  • Single-molecule fluorescence

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    Bharill, S., Chen, C., Stevens, B., Kaur, J., Smilansky, Z., Mandecki, W., Gryczynski, I., Gryczynski, Z., Cooperman, B. S., & Goldman, Y. E. (2011). Enhancement of single-molecule fluorescence signals by colloidal silver nanoparticles in studies of protein translation. ACS Nano, 5(1), 399-407. https://doi.org/10.1021/nn101839t