Enhancement of single-molecule fluorescence signals by colloidal silver nanoparticles in studies of protein translation

Shashank Bharill, Chunlai Chen, Benjamin Stevens, Jaskiran Kaur, Zeev Smilansky, Wlodek Mandecki, Ignacy Gryczynski, Zygmunt Gryczynski, Barry S. Cooperman, Yale E. Goldman

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Metal-enhanced fluorescence (MEF) increased total photon emission of Cy3- and Cy5-labeled ribosomal initiation complexes near 50 nm silver particles 4- and 5.5-fold, respectively. Fluorescence intensity fluctuations above shot noise, at 0.1-5 Hz, were greater on silver particles. Overall signal-to-noise ratio was similar or slightly improved near the particles. Proximity to silver particles did not compromise ribosome function, as measured by codon-dependent binding of fluorescent tRNA, dynamics of fluorescence resonance energy transfer between adjacent tRNAs in the ribosome, and tRNA translocation induced by elongation factor G.

Original languageEnglish
Pages (from-to)399-407
Number of pages9
JournalACS Nano
Volume5
Issue number1
DOIs
StatePublished - 25 Jan 2011

Fingerprint

Transfer RNA
Silver
Fluorescence
silver
Nanoparticles
ribosomes
proteins
Proteins
fluorescence
nanoparticles
Molecules
augmentation
Peptide Elongation Factor G
molecules
Shot noise
Elongation
Signal to noise ratio
shot noise
resonance fluorescence
Photons

Keywords

  • Metal-enhanced fluorescence
  • Protein translation
  • Ribosome
  • Silver colloids
  • Single-molecule fluorescence

Cite this

Bharill, S., Chen, C., Stevens, B., Kaur, J., Smilansky, Z., Mandecki, W., ... Goldman, Y. E. (2011). Enhancement of single-molecule fluorescence signals by colloidal silver nanoparticles in studies of protein translation. ACS Nano, 5(1), 399-407. https://doi.org/10.1021/nn101839t
Bharill, Shashank ; Chen, Chunlai ; Stevens, Benjamin ; Kaur, Jaskiran ; Smilansky, Zeev ; Mandecki, Wlodek ; Gryczynski, Ignacy ; Gryczynski, Zygmunt ; Cooperman, Barry S. ; Goldman, Yale E. / Enhancement of single-molecule fluorescence signals by colloidal silver nanoparticles in studies of protein translation. In: ACS Nano. 2011 ; Vol. 5, No. 1. pp. 399-407.
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Bharill, S, Chen, C, Stevens, B, Kaur, J, Smilansky, Z, Mandecki, W, Gryczynski, I, Gryczynski, Z, Cooperman, BS & Goldman, YE 2011, 'Enhancement of single-molecule fluorescence signals by colloidal silver nanoparticles in studies of protein translation', ACS Nano, vol. 5, no. 1, pp. 399-407. https://doi.org/10.1021/nn101839t

Enhancement of single-molecule fluorescence signals by colloidal silver nanoparticles in studies of protein translation. / Bharill, Shashank; Chen, Chunlai; Stevens, Benjamin; Kaur, Jaskiran; Smilansky, Zeev; Mandecki, Wlodek; Gryczynski, Ignacy; Gryczynski, Zygmunt; Cooperman, Barry S.; Goldman, Yale E.

In: ACS Nano, Vol. 5, No. 1, 25.01.2011, p. 399-407.

Research output: Contribution to journalArticle

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AU - Bharill, Shashank

AU - Chen, Chunlai

AU - Stevens, Benjamin

AU - Kaur, Jaskiran

AU - Smilansky, Zeev

AU - Mandecki, Wlodek

AU - Gryczynski, Ignacy

AU - Gryczynski, Zygmunt

AU - Cooperman, Barry S.

AU - Goldman, Yale E.

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