Abstract
Metal-enhanced fluorescence (MEF) increased total photon emission of Cy3- and Cy5-labeled ribosomal initiation complexes near 50 nm silver particles 4- and 5.5-fold, respectively. Fluorescence intensity fluctuations above shot noise, at 0.1-5 Hz, were greater on silver particles. Overall signal-to-noise ratio was similar or slightly improved near the particles. Proximity to silver particles did not compromise ribosome function, as measured by codon-dependent binding of fluorescent tRNA, dynamics of fluorescence resonance energy transfer between adjacent tRNAs in the ribosome, and tRNA translocation induced by elongation factor G.
Original language | English |
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Pages (from-to) | 399-407 |
Number of pages | 9 |
Journal | ACS Nano |
Volume | 5 |
Issue number | 1 |
DOIs | |
State | Published - 25 Jan 2011 |
Keywords
- Metal-enhanced fluorescence
- Protein translation
- Ribosome
- Silver colloids
- Single-molecule fluorescence