Enhancement of single-molecule fluorescence signals by colloidal silver nanoparticles in studies of protein translation

Shashank Bharill; Chunlai Chen; Benjamin Stevens; Jaskiran Kaur; Zeev Smilansky; Wlodek Mandecki; Ignacy Gryczynski; Zygmunt Gryczynski; Barry S. Cooperman; Yale E. Goldman

doi:10.1021/nn101839t

Enhancement of single-molecule fluorescence signals by colloidal silver nanoparticles in studies of protein translation

Shashank Bharill, Chunlai Chen, Benjamin Stevens, Jaskiran Kaur, Zeev Smilansky, Wlodek Mandecki, Ignacy Gryczynski, Zygmunt Gryczynski, Barry S. Cooperman, Yale E. Goldman

Microbiology, Immunology & Genetics

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

Metal-enhanced fluorescence (MEF) increased total photon emission of Cy3- and Cy5-labeled ribosomal initiation complexes near 50 nm silver particles 4- and 5.5-fold, respectively. Fluorescence intensity fluctuations above shot noise, at 0.1-5 Hz, were greater on silver particles. Overall signal-to-noise ratio was similar or slightly improved near the particles. Proximity to silver particles did not compromise ribosome function, as measured by codon-dependent binding of fluorescent tRNA, dynamics of fluorescence resonance energy transfer between adjacent tRNAs in the ribosome, and tRNA translocation induced by elongation factor G.

Original language	English
Pages (from-to)	399-407
Number of pages	9
Journal	ACS Nano
Volume	5
Issue number	1
DOIs	https://doi.org/10.1021/nn101839t
State	Published - 25 Jan 2011

Keywords

Metal-enhanced fluorescence
Protein translation
Ribosome
Silver colloids
Single-molecule fluorescence

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10.1021/nn101839t

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abstract = "Metal-enhanced fluorescence (MEF) increased total photon emission of Cy3- and Cy5-labeled ribosomal initiation complexes near 50 nm silver particles 4- and 5.5-fold, respectively. Fluorescence intensity fluctuations above shot noise, at 0.1-5 Hz, were greater on silver particles. Overall signal-to-noise ratio was similar or slightly improved near the particles. Proximity to silver particles did not compromise ribosome function, as measured by codon-dependent binding of fluorescent tRNA, dynamics of fluorescence resonance energy transfer between adjacent tRNAs in the ribosome, and tRNA translocation induced by elongation factor G.",

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T1 - Enhancement of single-molecule fluorescence signals by colloidal silver nanoparticles in studies of protein translation

AU - Bharill, Shashank

AU - Chen, Chunlai

AU - Stevens, Benjamin

AU - Kaur, Jaskiran

AU - Smilansky, Zeev

AU - Mandecki, Wlodek

AU - Gryczynski, Ignacy

AU - Gryczynski, Zygmunt

AU - Cooperman, Barry S.

AU - Goldman, Yale E.

PY - 2011/1/25

Y1 - 2011/1/25

N2 - Metal-enhanced fluorescence (MEF) increased total photon emission of Cy3- and Cy5-labeled ribosomal initiation complexes near 50 nm silver particles 4- and 5.5-fold, respectively. Fluorescence intensity fluctuations above shot noise, at 0.1-5 Hz, were greater on silver particles. Overall signal-to-noise ratio was similar or slightly improved near the particles. Proximity to silver particles did not compromise ribosome function, as measured by codon-dependent binding of fluorescent tRNA, dynamics of fluorescence resonance energy transfer between adjacent tRNAs in the ribosome, and tRNA translocation induced by elongation factor G.

AB - Metal-enhanced fluorescence (MEF) increased total photon emission of Cy3- and Cy5-labeled ribosomal initiation complexes near 50 nm silver particles 4- and 5.5-fold, respectively. Fluorescence intensity fluctuations above shot noise, at 0.1-5 Hz, were greater on silver particles. Overall signal-to-noise ratio was similar or slightly improved near the particles. Proximity to silver particles did not compromise ribosome function, as measured by codon-dependent binding of fluorescent tRNA, dynamics of fluorescence resonance energy transfer between adjacent tRNAs in the ribosome, and tRNA translocation induced by elongation factor G.

KW - Metal-enhanced fluorescence

KW - Protein translation

KW - Ribosome

KW - Silver colloids

KW - Single-molecule fluorescence

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Enhancement of single-molecule fluorescence signals by colloidal silver nanoparticles in studies of protein translation

Abstract

Keywords

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