Enhancement of anti-tumor effect of particulate vaccine delivery system by 'bacteriomimetic' CpG functionalization of poly-lactic-co-glycolic acid nanoparticles

Rutika A. Kokate, Sanjay I. Thamake, Pankaj Chaudhary, Brittney Mott, Sangram Limbaji Raut, Jamboor K. Vishwanatha, Harlan Jones

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Abstract

Aim: Low immunogenicity remains a major obstacle in realizing the full potential of cancer vaccines. In this study, we evaluated CpG-coated tumor antigen (Tag)-encapsulating 'bacteriomimetic' nanoparticles (CpG-nanoparticle [NP]-Tag NPs) as an approach to enhance anti-tumor immunity. Materials & methods: CpG-NP-Tag NPs were synthesized, characterized for their physicochemical properties and tested in vivo. Results: We found CpG predosing followed by intraperitoneal (IP) immunization with CpG-NP-Tag NPs significantly attenuated tumor growth in female BALB/c mice compared with respective controls. Histopathological and Immunofluorescence data revealed CpG-NP-Tag tumors had lower proliferation, higher apoptotic activity, greater CD4+ and CD8+ T cell infiltration as well as higher IFN-γ levels as compared with control groups. Conclusion: Our findings suggest CpG-NP-Tag NPs can enhance anti-tumor effect of nanoparticulate tumor vaccination system.

Original languageEnglish
Pages (from-to)915-929
Number of pages15
JournalNanomedicine
Volume10
Issue number6
DOIs
StatePublished - 1 Mar 2015

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Vaccines
vaccine
Neoplasm Antigens
tumor
Nanoparticles
Tumors
Acids
Antigens
acid
antigen
immunity
subversion
vaccination
proliferation
Neoplasms
cancer
Immunization
Cancer Vaccines
T-cells
Group

Keywords

  • NP
  • antigen
  • breast cancer
  • immunotherapy
  • nanoparticle
  • vaccines

Cite this

@article{d05cff208c414b268190c982dfdc5d51,
title = "Enhancement of anti-tumor effect of particulate vaccine delivery system by 'bacteriomimetic' CpG functionalization of poly-lactic-co-glycolic acid nanoparticles",
abstract = "Aim: Low immunogenicity remains a major obstacle in realizing the full potential of cancer vaccines. In this study, we evaluated CpG-coated tumor antigen (Tag)-encapsulating 'bacteriomimetic' nanoparticles (CpG-nanoparticle [NP]-Tag NPs) as an approach to enhance anti-tumor immunity. Materials & methods: CpG-NP-Tag NPs were synthesized, characterized for their physicochemical properties and tested in vivo. Results: We found CpG predosing followed by intraperitoneal (IP) immunization with CpG-NP-Tag NPs significantly attenuated tumor growth in female BALB/c mice compared with respective controls. Histopathological and Immunofluorescence data revealed CpG-NP-Tag tumors had lower proliferation, higher apoptotic activity, greater CD4+ and CD8+ T cell infiltration as well as higher IFN-γ levels as compared with control groups. Conclusion: Our findings suggest CpG-NP-Tag NPs can enhance anti-tumor effect of nanoparticulate tumor vaccination system.",
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author = "Kokate, {Rutika A.} and Thamake, {Sanjay I.} and Pankaj Chaudhary and Brittney Mott and Raut, {Sangram Limbaji} and Vishwanatha, {Jamboor K.} and Harlan Jones",
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doi = "10.2217/nnm.14.144",
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journal = "Nanomedicine",
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Enhancement of anti-tumor effect of particulate vaccine delivery system by 'bacteriomimetic' CpG functionalization of poly-lactic-co-glycolic acid nanoparticles. / Kokate, Rutika A.; Thamake, Sanjay I.; Chaudhary, Pankaj; Mott, Brittney; Raut, Sangram Limbaji; Vishwanatha, Jamboor K.; Jones, Harlan.

In: Nanomedicine, Vol. 10, No. 6, 01.03.2015, p. 915-929.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Enhancement of anti-tumor effect of particulate vaccine delivery system by 'bacteriomimetic' CpG functionalization of poly-lactic-co-glycolic acid nanoparticles

AU - Kokate, Rutika A.

AU - Thamake, Sanjay I.

AU - Chaudhary, Pankaj

AU - Mott, Brittney

AU - Raut, Sangram Limbaji

AU - Vishwanatha, Jamboor K.

AU - Jones, Harlan

PY - 2015/3/1

Y1 - 2015/3/1

N2 - Aim: Low immunogenicity remains a major obstacle in realizing the full potential of cancer vaccines. In this study, we evaluated CpG-coated tumor antigen (Tag)-encapsulating 'bacteriomimetic' nanoparticles (CpG-nanoparticle [NP]-Tag NPs) as an approach to enhance anti-tumor immunity. Materials & methods: CpG-NP-Tag NPs were synthesized, characterized for their physicochemical properties and tested in vivo. Results: We found CpG predosing followed by intraperitoneal (IP) immunization with CpG-NP-Tag NPs significantly attenuated tumor growth in female BALB/c mice compared with respective controls. Histopathological and Immunofluorescence data revealed CpG-NP-Tag tumors had lower proliferation, higher apoptotic activity, greater CD4+ and CD8+ T cell infiltration as well as higher IFN-γ levels as compared with control groups. Conclusion: Our findings suggest CpG-NP-Tag NPs can enhance anti-tumor effect of nanoparticulate tumor vaccination system.

AB - Aim: Low immunogenicity remains a major obstacle in realizing the full potential of cancer vaccines. In this study, we evaluated CpG-coated tumor antigen (Tag)-encapsulating 'bacteriomimetic' nanoparticles (CpG-nanoparticle [NP]-Tag NPs) as an approach to enhance anti-tumor immunity. Materials & methods: CpG-NP-Tag NPs were synthesized, characterized for their physicochemical properties and tested in vivo. Results: We found CpG predosing followed by intraperitoneal (IP) immunization with CpG-NP-Tag NPs significantly attenuated tumor growth in female BALB/c mice compared with respective controls. Histopathological and Immunofluorescence data revealed CpG-NP-Tag tumors had lower proliferation, higher apoptotic activity, greater CD4+ and CD8+ T cell infiltration as well as higher IFN-γ levels as compared with control groups. Conclusion: Our findings suggest CpG-NP-Tag NPs can enhance anti-tumor effect of nanoparticulate tumor vaccination system.

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