Enhancement of anti-tumor effect of particulate vaccine delivery system by 'bacteriomimetic' CpG functionalization of poly-lactic-co-glycolic acid nanoparticles

Rutika A. Kokate; Sanjay I. Thamake; Pankaj Chaudhary; Brittney Mott; Sangram Raut; Jamboor K. Vishwanatha; Harlan P. Jones

doi:10.2217/nnm.14.144

Enhancement of anti-tumor effect of particulate vaccine delivery system by 'bacteriomimetic' CpG functionalization of poly-lactic-co-glycolic acid nanoparticles

Rutika A. Kokate, Sanjay I. Thamake, Pankaj Chaudhary, Brittney Mott, Sangram Raut, Jamboor K. Vishwanatha, Harlan P. Jones

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Aim: Low immunogenicity remains a major obstacle in realizing the full potential of cancer vaccines. In this study, we evaluated CpG-coated tumor antigen (Tag)-encapsulating 'bacteriomimetic' nanoparticles (CpG-nanoparticle [NP]-Tag NPs) as an approach to enhance anti-tumor immunity. Materials & methods: CpG-NP-Tag NPs were synthesized, characterized for their physicochemical properties and tested in vivo. Results: We found CpG predosing followed by intraperitoneal (IP) immunization with CpG-NP-Tag NPs significantly attenuated tumor growth in female BALB/c mice compared with respective controls. Histopathological and Immunofluorescence data revealed CpG-NP-Tag tumors had lower proliferation, higher apoptotic activity, greater CD4⁺ and CD8⁺ T cell infiltration as well as higher IFN-γ levels as compared with control groups. Conclusion: Our findings suggest CpG-NP-Tag NPs can enhance anti-tumor effect of nanoparticulate tumor vaccination system.

Original language	English
Pages (from-to)	915-929
Number of pages	15
Journal	Nanomedicine
Volume	10
Issue number	6
DOIs	https://doi.org/10.2217/nnm.14.144
State	Published - 1 Mar 2015

Keywords

NP
antigen
breast cancer
immunotherapy
nanoparticle
vaccines

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title = "Enhancement of anti-tumor effect of particulate vaccine delivery system by 'bacteriomimetic' CpG functionalization of poly-lactic-co-glycolic acid nanoparticles",

abstract = "Aim: Low immunogenicity remains a major obstacle in realizing the full potential of cancer vaccines. In this study, we evaluated CpG-coated tumor antigen (Tag)-encapsulating 'bacteriomimetic' nanoparticles (CpG-nanoparticle [NP]-Tag NPs) as an approach to enhance anti-tumor immunity. Materials & methods: CpG-NP-Tag NPs were synthesized, characterized for their physicochemical properties and tested in vivo. Results: We found CpG predosing followed by intraperitoneal (IP) immunization with CpG-NP-Tag NPs significantly attenuated tumor growth in female BALB/c mice compared with respective controls. Histopathological and Immunofluorescence data revealed CpG-NP-Tag tumors had lower proliferation, higher apoptotic activity, greater CD4+ and CD8+ T cell infiltration as well as higher IFN-γ levels as compared with control groups. Conclusion: Our findings suggest CpG-NP-Tag NPs can enhance anti-tumor effect of nanoparticulate tumor vaccination system.",

keywords = "NP, antigen, breast cancer, immunotherapy, nanoparticle, vaccines",

author = "Kokate, {Rutika A.} and Thamake, {Sanjay I.} and Pankaj Chaudhary and Brittney Mott and Sangram Raut and Vishwanatha, {Jamboor K.} and Jones, {Harlan P.}",

note = "Publisher Copyright: {\textcopyright} 2015 Future Medicine Ltd.",

year = "2015",

month = mar,

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doi = "10.2217/nnm.14.144",

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T1 - Enhancement of anti-tumor effect of particulate vaccine delivery system by 'bacteriomimetic' CpG functionalization of poly-lactic-co-glycolic acid nanoparticles

AU - Kokate, Rutika A.

AU - Thamake, Sanjay I.

AU - Chaudhary, Pankaj

AU - Mott, Brittney

AU - Raut, Sangram

AU - Vishwanatha, Jamboor K.

AU - Jones, Harlan P.

PY - 2015/3/1

Y1 - 2015/3/1

N2 - Aim: Low immunogenicity remains a major obstacle in realizing the full potential of cancer vaccines. In this study, we evaluated CpG-coated tumor antigen (Tag)-encapsulating 'bacteriomimetic' nanoparticles (CpG-nanoparticle [NP]-Tag NPs) as an approach to enhance anti-tumor immunity. Materials & methods: CpG-NP-Tag NPs were synthesized, characterized for their physicochemical properties and tested in vivo. Results: We found CpG predosing followed by intraperitoneal (IP) immunization with CpG-NP-Tag NPs significantly attenuated tumor growth in female BALB/c mice compared with respective controls. Histopathological and Immunofluorescence data revealed CpG-NP-Tag tumors had lower proliferation, higher apoptotic activity, greater CD4+ and CD8+ T cell infiltration as well as higher IFN-γ levels as compared with control groups. Conclusion: Our findings suggest CpG-NP-Tag NPs can enhance anti-tumor effect of nanoparticulate tumor vaccination system.

AB - Aim: Low immunogenicity remains a major obstacle in realizing the full potential of cancer vaccines. In this study, we evaluated CpG-coated tumor antigen (Tag)-encapsulating 'bacteriomimetic' nanoparticles (CpG-nanoparticle [NP]-Tag NPs) as an approach to enhance anti-tumor immunity. Materials & methods: CpG-NP-Tag NPs were synthesized, characterized for their physicochemical properties and tested in vivo. Results: We found CpG predosing followed by intraperitoneal (IP) immunization with CpG-NP-Tag NPs significantly attenuated tumor growth in female BALB/c mice compared with respective controls. Histopathological and Immunofluorescence data revealed CpG-NP-Tag tumors had lower proliferation, higher apoptotic activity, greater CD4+ and CD8+ T cell infiltration as well as higher IFN-γ levels as compared with control groups. Conclusion: Our findings suggest CpG-NP-Tag NPs can enhance anti-tumor effect of nanoparticulate tumor vaccination system.

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KW - antigen

KW - breast cancer

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KW - nanoparticle

KW - vaccines

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Enhancement of anti-tumor effect of particulate vaccine delivery system by 'bacteriomimetic' CpG functionalization of poly-lactic-co-glycolic acid nanoparticles

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