Enhancement of anti-tumor effect of particulate vaccine delivery system by 'bacteriomimetic' CpG functionalization of poly-lactic-co-glycolic acid nanoparticles

Rutika A. Kokate, Sanjay I. Thamake, Pankaj Chaudhary, Brittney Mott, Sangram Raut, Jamboor K. Vishwanatha, Harlan P. Jones

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Aim: Low immunogenicity remains a major obstacle in realizing the full potential of cancer vaccines. In this study, we evaluated CpG-coated tumor antigen (Tag)-encapsulating 'bacteriomimetic' nanoparticles (CpG-nanoparticle [NP]-Tag NPs) as an approach to enhance anti-tumor immunity. Materials & methods: CpG-NP-Tag NPs were synthesized, characterized for their physicochemical properties and tested in vivo. Results: We found CpG predosing followed by intraperitoneal (IP) immunization with CpG-NP-Tag NPs significantly attenuated tumor growth in female BALB/c mice compared with respective controls. Histopathological and Immunofluorescence data revealed CpG-NP-Tag tumors had lower proliferation, higher apoptotic activity, greater CD4+ and CD8+ T cell infiltration as well as higher IFN-γ levels as compared with control groups. Conclusion: Our findings suggest CpG-NP-Tag NPs can enhance anti-tumor effect of nanoparticulate tumor vaccination system.

Original languageEnglish
Pages (from-to)915-929
Number of pages15
JournalNanomedicine
Volume10
Issue number6
DOIs
StatePublished - 1 Mar 2015

Keywords

  • NP
  • antigen
  • breast cancer
  • immunotherapy
  • nanoparticle
  • vaccines

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