TY - JOUR
T1 - Enhanced resolution of anisotropic rotational diffusion by multi-wavelength frequency-domain fluorometry and global analysis
AU - Gryczynski, Ignacy
AU - Cherek, Henryk
AU - Laczko, Gabor
AU - Lakowicz, Joseph R.
N1 - Funding Information:
Supportedb y grantsP CM-8210872a nd DMB-0850283f5r omt heN ationalS cienceF oundationa nd GM-2931 8a nd GM-35 154f rom the NationalI nsti-tuteso f Health.J RL offersh is specialt hankst o the National ScienceF oundationf or supportingd evel-opmento f the frequency-domaimn ethod.H C was on leave from Nicholas Copernicus University, Torun, Poland with partial support from CPBP 01.06.2.03(P oland). IG waso n leavef rom University of Gdansk,I nstituteo f ExperimentaPl hysics, Gdansk, Poland, with partial supportf rom CPBP 01.06.2.01(P oland).
PY - 1987/4/3
Y1 - 1987/4/3
N2 - Frequency-dependent differential polarized phase and modulation data were used to measure time-dependent anisotropy decays of 9-aminoacridine and Y-base. The data were obtained at various excitation wavelengths, at which the fundamental anisotropies (r0) varied from -0.17 to 0.373. The different values of r0 result in different contributions of the multiple correlation times to the anisotropy decay. The multi-wavelength data were analyzed globally, revealing both molecules to be anisotropic rotators. The rotational motions of Y-base are more anisotropic than those of 9-aminoacridine. In propylene glycol at -5 ° C the two correlation times for Y-base were found to be 2.1 and 12.1 ns. For 9-aminoacridine the correlation times were 9.5 and 14.1 ns.
AB - Frequency-dependent differential polarized phase and modulation data were used to measure time-dependent anisotropy decays of 9-aminoacridine and Y-base. The data were obtained at various excitation wavelengths, at which the fundamental anisotropies (r0) varied from -0.17 to 0.373. The different values of r0 result in different contributions of the multiple correlation times to the anisotropy decay. The multi-wavelength data were analyzed globally, revealing both molecules to be anisotropic rotators. The rotational motions of Y-base are more anisotropic than those of 9-aminoacridine. In propylene glycol at -5 ° C the two correlation times for Y-base were found to be 2.1 and 12.1 ns. For 9-aminoacridine the correlation times were 9.5 and 14.1 ns.
UR - http://www.scopus.com/inward/record.url?scp=0345541542&partnerID=8YFLogxK
U2 - 10.1016/0009-2614(87)85141-2
DO - 10.1016/0009-2614(87)85141-2
M3 - Article
AN - SCOPUS:0345541542
SN - 0009-2614
VL - 135
SP - 193
EP - 199
JO - Chemical Physics Letters
JF - Chemical Physics Letters
IS - 3
ER -