Enhanced immune response to DNA-based HPV16L1 vaccination by costimulatory molecule B7-2

Jin Zheng, Lusheng Si, Jianmin Song, Xiangle Sun, Jun Yu, Yili Wang

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

We have investigated whether co-injection of DNA encoding the costimulatory molecule B7-2 augments immune response to the major capsid protein L1 of the high-risk human papillomavirus type 16 (HPV16L1). While immunoglobulin G (IgG) specific to HPV16L1 was detected in sera from mice injected intramuscularly with pcDNA-L1 that encodes HPV16L1, a significantly increased level of IgG was found in sera from mice immunised with pcDNA-L1 in conjunction with pLXHDmB7-2 DNA. Levels of IgG in the anti-sera were correlated with the inhibitory activity of the murine erythrocyte hemagglutination caused by the virus-like particles (VLP) and the binding of VLP to HeLa cells. Moreover, splenic cells isolated from mice co-injected with pLXHDmB7-2 had stronger proliferation and more IFN-γ producing T cells (CD4+ and CD8+) when stimulated with HPV16 VLP compared with cells from mice that had received pcDNA-L1 alone and mice of the control groups. Furthermore, in footpad swelling test, mice co-immunised with pLXHDmB7-2 had greater skin thickness over those immunised with pcDNA-L1 alone or control mice. We conclude that co-injection of DNA encoding B7-2 can enhance both humoral and cellular immune responses elicited by DNA-based vaccination against HPV16 infection in mice.

Original languageEnglish
Pages (from-to)61-65
Number of pages5
JournalAntiviral Research
Volume59
Issue number1
DOIs
StatePublished - 1 Jun 2003

Keywords

  • Costimulatory molecule
  • DNA-vaccination
  • Human papillomavirus

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