Enhanced fluorescent immunoassays on silver fractal-like structures

Tanya Shtoyko, Evgenia G. Matveeva, I. Fen Chang, Zygmunt Gryczynski, Ewa Goldys, Ignacy Gryczynski

Research output: Contribution to journalArticle

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Abstract

Using the effect of the fluorescence enhancement in close proximity to metal nanostructures, we have been able to demonstrate ultrasensitive immunoassays suitable for the detection of biomarkers. Silver fractal-like structures have been grown by electrochemical reduction of silver on the surface of glass slides. A model immunoassay was performed on the slide surface with rabbit IgG (antigen) noncovalently immobilized on the slide, and rhodamine red-X-labeled antirabbit IgG conjugate was subsequently bound to the immobilized antigen. The fluorescence signal was measured from the glass-fractal's surface using a confocal microscope, and the images were compared to the images from the same surface not coated with fractals. Our results showed significant enhancement (more than 100-fold) of the signal detected on fractals compared to bare glass. We thus demonstrate that such fractal-like structures can assist in improving the signals from assays used in medical diagnostics, especially those for analytes with molecular weight under 100 kDa.

Original languageEnglish
Pages (from-to)1962-1966
Number of pages5
JournalAnalytical Chemistry
Volume80
Issue number6
DOIs
StatePublished - 15 Mar 2008

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Silver
Fractals
Glass
Immunoglobulin G
Fluorescence
Antigens
Rhodamines
Biomarkers
Assays
Nanostructures
Microscopes
Metals
Molecular weight

Cite this

Shtoyko, Tanya ; Matveeva, Evgenia G. ; Chang, I. Fen ; Gryczynski, Zygmunt ; Goldys, Ewa ; Gryczynski, Ignacy. / Enhanced fluorescent immunoassays on silver fractal-like structures. In: Analytical Chemistry. 2008 ; Vol. 80, No. 6. pp. 1962-1966.
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Enhanced fluorescent immunoassays on silver fractal-like structures. / Shtoyko, Tanya; Matveeva, Evgenia G.; Chang, I. Fen; Gryczynski, Zygmunt; Goldys, Ewa; Gryczynski, Ignacy.

In: Analytical Chemistry, Vol. 80, No. 6, 15.03.2008, p. 1962-1966.

Research output: Contribution to journalArticle

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AU - Shtoyko, Tanya

AU - Matveeva, Evgenia G.

AU - Chang, I. Fen

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AB - Using the effect of the fluorescence enhancement in close proximity to metal nanostructures, we have been able to demonstrate ultrasensitive immunoassays suitable for the detection of biomarkers. Silver fractal-like structures have been grown by electrochemical reduction of silver on the surface of glass slides. A model immunoassay was performed on the slide surface with rabbit IgG (antigen) noncovalently immobilized on the slide, and rhodamine red-X-labeled antirabbit IgG conjugate was subsequently bound to the immobilized antigen. The fluorescence signal was measured from the glass-fractal's surface using a confocal microscope, and the images were compared to the images from the same surface not coated with fractals. Our results showed significant enhancement (more than 100-fold) of the signal detected on fractals compared to bare glass. We thus demonstrate that such fractal-like structures can assist in improving the signals from assays used in medical diagnostics, especially those for analytes with molecular weight under 100 kDa.

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