Enhanced fluorescence of labeled proteins coated on silver nanoparticles

Joanna Lukomska, Evgenia G. Matveeva, Ignacy Gryczynski, Robert W. Flower, Joanna Malicka, Joseph R. Lakowicz, Slawomir Makowiec, Zygmunt Gryczynski

Research output: Contribution to journalConference article

Abstract

We have recently shown that metallic particles or colloids when deposited on the transparent surface can enhance fluorescence properties of nearby fluorophores. We obtained the fluorophore-metal colloid complexes that display significant fluorescence signal enhancement in solution. Silver nanoparticles (about 20-50 nm size) were synthesized as a stable yellow colloidal solution, and coated with proteins labeled with fluorophores. A several-fold amplification of the fluorescence signal in presence of colloid Ag nanoparticles in solution was observed. Such fluorophore-metal complex presents a unique opportunity for developing of new class of contrast agents for optical imaging and fluorescence based sensing. Solution of silver nanoparticles with enhanced fluorescence can be used in various assays such as DNA hybridization or immunoassays for high sensitivity detection.

Original languageEnglish
Article number06
Pages (from-to)35-41
Number of pages7
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume5703
DOIs
StatePublished - 16 Aug 2005
EventPlasmonics in Biology and Medicine II - San Jose, CA, United States
Duration: 24 Jan 200525 Jan 2005

Fingerprint

Silver
Nanoparticles
Fluorophores
Fluorescence
silver
proteins
Proteins
Colloids
fluorescence
nanoparticles
colloids
Coordination Complexes
immunoassay
Optical Imaging
Metal complexes
Immunoassay
metals
Contrast Media
Particles (particulate matter)
Amplification

Keywords

  • Enhanced fluorescence
  • Fluorescence assays
  • Nano-size metallic particles
  • Protein

Cite this

Lukomska, Joanna ; Matveeva, Evgenia G. ; Gryczynski, Ignacy ; Flower, Robert W. ; Malicka, Joanna ; Lakowicz, Joseph R. ; Makowiec, Slawomir ; Gryczynski, Zygmunt. / Enhanced fluorescence of labeled proteins coated on silver nanoparticles. In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2005 ; Vol. 5703. pp. 35-41.
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Enhanced fluorescence of labeled proteins coated on silver nanoparticles. / Lukomska, Joanna; Matveeva, Evgenia G.; Gryczynski, Ignacy; Flower, Robert W.; Malicka, Joanna; Lakowicz, Joseph R.; Makowiec, Slawomir; Gryczynski, Zygmunt.

In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 5703, 06, 16.08.2005, p. 35-41.

Research output: Contribution to journalConference article

TY - JOUR

T1 - Enhanced fluorescence of labeled proteins coated on silver nanoparticles

AU - Lukomska, Joanna

AU - Matveeva, Evgenia G.

AU - Gryczynski, Ignacy

AU - Flower, Robert W.

AU - Malicka, Joanna

AU - Lakowicz, Joseph R.

AU - Makowiec, Slawomir

AU - Gryczynski, Zygmunt

PY - 2005/8/16

Y1 - 2005/8/16

N2 - We have recently shown that metallic particles or colloids when deposited on the transparent surface can enhance fluorescence properties of nearby fluorophores. We obtained the fluorophore-metal colloid complexes that display significant fluorescence signal enhancement in solution. Silver nanoparticles (about 20-50 nm size) were synthesized as a stable yellow colloidal solution, and coated with proteins labeled with fluorophores. A several-fold amplification of the fluorescence signal in presence of colloid Ag nanoparticles in solution was observed. Such fluorophore-metal complex presents a unique opportunity for developing of new class of contrast agents for optical imaging and fluorescence based sensing. Solution of silver nanoparticles with enhanced fluorescence can be used in various assays such as DNA hybridization or immunoassays for high sensitivity detection.

AB - We have recently shown that metallic particles or colloids when deposited on the transparent surface can enhance fluorescence properties of nearby fluorophores. We obtained the fluorophore-metal colloid complexes that display significant fluorescence signal enhancement in solution. Silver nanoparticles (about 20-50 nm size) were synthesized as a stable yellow colloidal solution, and coated with proteins labeled with fluorophores. A several-fold amplification of the fluorescence signal in presence of colloid Ag nanoparticles in solution was observed. Such fluorophore-metal complex presents a unique opportunity for developing of new class of contrast agents for optical imaging and fluorescence based sensing. Solution of silver nanoparticles with enhanced fluorescence can be used in various assays such as DNA hybridization or immunoassays for high sensitivity detection.

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