Enhanced fluorescence of labeled proteins coated on silver nanoparticles

Joanna Lukomska; Evgenia G. Matveeva; Ignacy Gryczynski; Robert W. Flower; Joanna Malicka; Joseph R. Lakowicz; Slawomir Makowiec; Zygmunt Gryczynski

doi:10.1117/12.593426

Enhanced fluorescence of labeled proteins coated on silver nanoparticles

Joanna Lukomska, Evgenia G. Matveeva, Ignacy Gryczynski, Robert W. Flower, Joanna Malicka, Joseph R. Lakowicz, Slawomir Makowiec, Zygmunt Gryczynski

Research output: Contribution to journal › Conference article › peer-review

Abstract

We have recently shown that metallic particles or colloids when deposited on the transparent surface can enhance fluorescence properties of nearby fluorophores. We obtained the fluorophore-metal colloid complexes that display significant fluorescence signal enhancement in solution. Silver nanoparticles (about 20-50 nm size) were synthesized as a stable yellow colloidal solution, and coated with proteins labeled with fluorophores. A several-fold amplification of the fluorescence signal in presence of colloid Ag nanoparticles in solution was observed. Such fluorophore-metal complex presents a unique opportunity for developing of new class of contrast agents for optical imaging and fluorescence based sensing. Solution of silver nanoparticles with enhanced fluorescence can be used in various assays such as DNA hybridization or immunoassays for high sensitivity detection.

Original language	English
Article number	06
Pages (from-to)	35-41
Number of pages	7
Journal	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	5703
DOIs	https://doi.org/10.1117/12.593426
State	Published - 16 Aug 2005
Event	Plasmonics in Biology and Medicine II - San Jose, CA, United States Duration: 24 Jan 2005 → 25 Jan 2005

Keywords

Enhanced fluorescence
Fluorescence assays
Nano-size metallic particles
Protein

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10.1117/12.593426

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abstract = "We have recently shown that metallic particles or colloids when deposited on the transparent surface can enhance fluorescence properties of nearby fluorophores. We obtained the fluorophore-metal colloid complexes that display significant fluorescence signal enhancement in solution. Silver nanoparticles (about 20-50 nm size) were synthesized as a stable yellow colloidal solution, and coated with proteins labeled with fluorophores. A several-fold amplification of the fluorescence signal in presence of colloid Ag nanoparticles in solution was observed. Such fluorophore-metal complex presents a unique opportunity for developing of new class of contrast agents for optical imaging and fluorescence based sensing. Solution of silver nanoparticles with enhanced fluorescence can be used in various assays such as DNA hybridization or immunoassays for high sensitivity detection.",

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AU - Lukomska, Joanna

AU - Matveeva, Evgenia G.

AU - Gryczynski, Ignacy

AU - Flower, Robert W.

AU - Malicka, Joanna

AU - Lakowicz, Joseph R.

AU - Makowiec, Slawomir

AU - Gryczynski, Zygmunt

PY - 2005/8/16

Y1 - 2005/8/16

N2 - We have recently shown that metallic particles or colloids when deposited on the transparent surface can enhance fluorescence properties of nearby fluorophores. We obtained the fluorophore-metal colloid complexes that display significant fluorescence signal enhancement in solution. Silver nanoparticles (about 20-50 nm size) were synthesized as a stable yellow colloidal solution, and coated with proteins labeled with fluorophores. A several-fold amplification of the fluorescence signal in presence of colloid Ag nanoparticles in solution was observed. Such fluorophore-metal complex presents a unique opportunity for developing of new class of contrast agents for optical imaging and fluorescence based sensing. Solution of silver nanoparticles with enhanced fluorescence can be used in various assays such as DNA hybridization or immunoassays for high sensitivity detection.

AB - We have recently shown that metallic particles or colloids when deposited on the transparent surface can enhance fluorescence properties of nearby fluorophores. We obtained the fluorophore-metal colloid complexes that display significant fluorescence signal enhancement in solution. Silver nanoparticles (about 20-50 nm size) were synthesized as a stable yellow colloidal solution, and coated with proteins labeled with fluorophores. A several-fold amplification of the fluorescence signal in presence of colloid Ag nanoparticles in solution was observed. Such fluorophore-metal complex presents a unique opportunity for developing of new class of contrast agents for optical imaging and fluorescence based sensing. Solution of silver nanoparticles with enhanced fluorescence can be used in various assays such as DNA hybridization or immunoassays for high sensitivity detection.

KW - Enhanced fluorescence

KW - Fluorescence assays

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Enhanced fluorescence of labeled proteins coated on silver nanoparticles

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Keywords

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