Abstract
We have recently shown that metallic particles or colloids when deposited on the transparent surface can enhance fluorescence properties of nearby fluorophores. We obtained the fluorophore-metal colloid complexes that display significant fluorescence signal enhancement in solution. Silver nanoparticles (about 20-50 nm size) were synthesized as a stable yellow colloidal solution, and coated with proteins labeled with fluorophores. A several-fold amplification of the fluorescence signal in presence of colloid Ag nanoparticles in solution was observed. Such fluorophore-metal complex presents a unique opportunity for developing of new class of contrast agents for optical imaging and fluorescence based sensing. Solution of silver nanoparticles with enhanced fluorescence can be used in various assays such as DNA hybridization or immunoassays for high sensitivity detection.
| Original language | English |
|---|---|
| Article number | 06 |
| Pages (from-to) | 35-41 |
| Number of pages | 7 |
| Journal | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
| Volume | 5703 |
| DOIs | |
| State | Published - 16 Aug 2005 |
| Event | Plasmonics in Biology and Medicine II - San Jose, CA, United States Duration: 24 Jan 2005 → 25 Jan 2005 |
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Keywords
- Enhanced fluorescence
- Fluorescence assays
- Nano-size metallic particles
- Protein
Cite this
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Enhanced fluorescence of labeled proteins coated on silver nanoparticles. / Lukomska, Joanna; Matveeva, Evgenia G.; Gryczynski, Ignacy; Flower, Robert W.; Malicka, Joanna; Lakowicz, Joseph R.; Makowiec, Slawomir; Gryczynski, Zygmunt.
In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 5703, 06, 16.08.2005, p. 35-41.Research output: Contribution to journal › Conference article
TY - JOUR
T1 - Enhanced fluorescence of labeled proteins coated on silver nanoparticles
AU - Lukomska, Joanna
AU - Matveeva, Evgenia G.
AU - Gryczynski, Ignacy
AU - Flower, Robert W.
AU - Malicka, Joanna
AU - Lakowicz, Joseph R.
AU - Makowiec, Slawomir
AU - Gryczynski, Zygmunt
PY - 2005/8/16
Y1 - 2005/8/16
N2 - We have recently shown that metallic particles or colloids when deposited on the transparent surface can enhance fluorescence properties of nearby fluorophores. We obtained the fluorophore-metal colloid complexes that display significant fluorescence signal enhancement in solution. Silver nanoparticles (about 20-50 nm size) were synthesized as a stable yellow colloidal solution, and coated with proteins labeled with fluorophores. A several-fold amplification of the fluorescence signal in presence of colloid Ag nanoparticles in solution was observed. Such fluorophore-metal complex presents a unique opportunity for developing of new class of contrast agents for optical imaging and fluorescence based sensing. Solution of silver nanoparticles with enhanced fluorescence can be used in various assays such as DNA hybridization or immunoassays for high sensitivity detection.
AB - We have recently shown that metallic particles or colloids when deposited on the transparent surface can enhance fluorescence properties of nearby fluorophores. We obtained the fluorophore-metal colloid complexes that display significant fluorescence signal enhancement in solution. Silver nanoparticles (about 20-50 nm size) were synthesized as a stable yellow colloidal solution, and coated with proteins labeled with fluorophores. A several-fold amplification of the fluorescence signal in presence of colloid Ag nanoparticles in solution was observed. Such fluorophore-metal complex presents a unique opportunity for developing of new class of contrast agents for optical imaging and fluorescence based sensing. Solution of silver nanoparticles with enhanced fluorescence can be used in various assays such as DNA hybridization or immunoassays for high sensitivity detection.
KW - Enhanced fluorescence
KW - Fluorescence assays
KW - Nano-size metallic particles
KW - Protein
UR - http://www.scopus.com/inward/record.url?scp=23244446983&partnerID=8YFLogxK
U2 - 10.1117/12.593426
DO - 10.1117/12.593426
M3 - Conference article
AN - SCOPUS:23244446983
VL - 5703
SP - 35
EP - 41
JO - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
JF - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
SN - 1605-7422
M1 - 06
ER -