Enhanced fluorescence from fluorophores on fractal silver surfaces

Alexandr Parfenov; Ignacy Gryczynski; Joanna Malicka; Chris D. Geddes; Joseph R. Lakowicz

doi:10.1021/jp022660r

Enhanced fluorescence from fluorophores on fractal silver surfaces

Alexandr Parfenov, Ignacy Gryczynski, Joanna Malicka, Chris D. Geddes, Joseph R. Lakowicz

Microbiology, Immunology & Genetics

Research output: Contribution to journal › Article › peer-review

180 Scopus citations

Abstract

Recent reports have shown enhanced fluorescence for fluorophores in close proximity to chemically deposited silver islands or colloids. To expand the usefulness of metal-enhanced fluorescence we tested fractal silver structures formed on, or near, silver electrodes by passage of electric currents. The emission intensity of fluorescein-labeled human serum albumin (FITC-HSA) was enhanced over 100-fold when adsorbed to the fractal silver structures as compared to glass. The amplitude-weighted lifetime is dramatically reduced to near 3 ps. Enhanced fluorescence was shown to result in selective observation of FITC-HSA over a fluorophore not attached to the silver surface. And finally, photostability measurements indicate 160-fold more photons are detectable from FITC-HSA on the fractal silver surface. These results suggest the use of in situ generated silver structures for metal-enhanced fluorescence.

Original language	English
Pages (from-to)	8829-8833
Number of pages	5
Journal	Journal of Physical Chemistry B
Volume	107
Issue number	34
DOIs	https://doi.org/10.1021/jp022660r
State	Published - 28 Aug 2003

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title = "Enhanced fluorescence from fluorophores on fractal silver surfaces",

abstract = "Recent reports have shown enhanced fluorescence for fluorophores in close proximity to chemically deposited silver islands or colloids. To expand the usefulness of metal-enhanced fluorescence we tested fractal silver structures formed on, or near, silver electrodes by passage of electric currents. The emission intensity of fluorescein-labeled human serum albumin (FITC-HSA) was enhanced over 100-fold when adsorbed to the fractal silver structures as compared to glass. The amplitude-weighted lifetime is dramatically reduced to near 3 ps. Enhanced fluorescence was shown to result in selective observation of FITC-HSA over a fluorophore not attached to the silver surface. And finally, photostability measurements indicate 160-fold more photons are detectable from FITC-HSA on the fractal silver surface. These results suggest the use of in situ generated silver structures for metal-enhanced fluorescence.",

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T1 - Enhanced fluorescence from fluorophores on fractal silver surfaces

AU - Parfenov, Alexandr

AU - Gryczynski, Ignacy

AU - Malicka, Joanna

AU - Geddes, Chris D.

AU - Lakowicz, Joseph R.

PY - 2003/8/28

Y1 - 2003/8/28

N2 - Recent reports have shown enhanced fluorescence for fluorophores in close proximity to chemically deposited silver islands or colloids. To expand the usefulness of metal-enhanced fluorescence we tested fractal silver structures formed on, or near, silver electrodes by passage of electric currents. The emission intensity of fluorescein-labeled human serum albumin (FITC-HSA) was enhanced over 100-fold when adsorbed to the fractal silver structures as compared to glass. The amplitude-weighted lifetime is dramatically reduced to near 3 ps. Enhanced fluorescence was shown to result in selective observation of FITC-HSA over a fluorophore not attached to the silver surface. And finally, photostability measurements indicate 160-fold more photons are detectable from FITC-HSA on the fractal silver surface. These results suggest the use of in situ generated silver structures for metal-enhanced fluorescence.

AB - Recent reports have shown enhanced fluorescence for fluorophores in close proximity to chemically deposited silver islands or colloids. To expand the usefulness of metal-enhanced fluorescence we tested fractal silver structures formed on, or near, silver electrodes by passage of electric currents. The emission intensity of fluorescein-labeled human serum albumin (FITC-HSA) was enhanced over 100-fold when adsorbed to the fractal silver structures as compared to glass. The amplitude-weighted lifetime is dramatically reduced to near 3 ps. Enhanced fluorescence was shown to result in selective observation of FITC-HSA over a fluorophore not attached to the silver surface. And finally, photostability measurements indicate 160-fold more photons are detectable from FITC-HSA on the fractal silver surface. These results suggest the use of in situ generated silver structures for metal-enhanced fluorescence.

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Enhanced fluorescence from fluorophores on fractal silver surfaces

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