Enhanced biodegradation of β- and δ-hexachlorocyclohexane in the presence of α- and α-isomers in contaminated soils

Manish Kumar, Pankaj Chaudhary, Manish Dwivedi, Ranjan Kumar, Debarati Paul, Rakesh K. Jain, Satyendra K. Garg, Ashwani Kumar

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

The chlorinated insecticide hexachlorocyclohexane (HCH) has been used extensively in the past, and contaminated sites are present throughout the world. Toward their bioremediation, we isolated a bacterium Pseudomonas aeruginosa ITRC-5 that mediates the degradation of all the four major isomers of HCH under aerobic conditions, both in liquid-culture and contaminated soils. In liquid-culture, the degradation of α- and γ-HCH is rapid and is accompanied with the release of 5.6 μmole chloride ions and 4.1 μmole CO2mole-1 HCH-isomer. The degradation of β- and δ5-isomers is slow, accompanied with the release of 0.9 μmole chloride ions μmole-1 HCH-isomer, and results in a transient metabolite 2,3,4,5,6-pentachlorocyclohexan-1-ol. The strain ITRC-5 also mediates the degradation of α-, β-, γ, and <5-isomers in contaminated soils, where degradation of otherwise persistent β- and <3-HCH is enhanced severalfold inthe presence of α- or γ-HCH. The degradation of soil-applied β- and <3-HCH under aerobic conditions has not been reported earlier. The isolate ITRC-5 therefore demonstrates potential for the bioremediation of HCH-wastes and contaminated soils.

Original languageEnglish
Pages (from-to)4005-4011
Number of pages7
JournalEnvironmental Science and Technology
Volume39
Issue number11
DOIs
StatePublished - 1 Jun 2005

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