Endothelin-1 distribution and basolateral secretion in the retinal pigment epithelium

Santosh Narayan, Anne Marie Brun, Thomas Yorio

Research output: Contribution to journalArticleResearchpeer-review

21 Citations (Scopus)

Abstract

Endothelins are a family of conserved vasoactive peptides that are widely expressed in different biological systems including the eye. In the cell culture model of retinal pigment epithelium, ARPE-19, the synthesis and secretion of endothelin-1 (ET-1) is regulated by cholinergics and TNF-α. In the present study we investigated the expression of ET-1 in RPE in situ, in rat and human eyes. Additionally, we have employed the human retinal pigment epithelial (ARPE-19) cells to delineate the apical and basolateral ET-1 expression and secretion by confocal microscopy and radioimmunoassay, respectively. Our results suggest a possible conservation of ET-1 expression predominantly in the mammalian RPE underlining its importance at this site. Additionally, our results suggest that constitutive ET-1 secretion is predominantly towards the basolateral side in cultured RPE possibly allowing ET-1 to activate its receptors located in the choroidal blood vessels and regulate retinal and choroidal blood flow.

Original languageEnglish
Pages (from-to)11-19
Number of pages9
JournalExperimental eye research
Volume79
Issue number1
DOIs
StatePublished - 1 Jul 2004

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Retinal Pigment Epithelium
Endothelin-1
Retinal Vessels
Retinal Pigments
Endothelins
Confocal Microscopy
Cholinergic Agents
Radioimmunoassay
Cell Culture Techniques
Peptides

Keywords

  • endothelin-1
  • polarity
  • retinal pigment epithelium

Cite this

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Endothelin-1 distribution and basolateral secretion in the retinal pigment epithelium. / Narayan, Santosh; Brun, Anne Marie; Yorio, Thomas.

In: Experimental eye research, Vol. 79, No. 1, 01.07.2004, p. 11-19.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Yorio, Thomas

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