Endothelin-1 distribution and basolateral secretion in the retinal pigment epithelium

Santosh Narayan; Anne Marie Brun; Thomas Yorio

doi:10.1016/j.exer.2004.03.002

Endothelin-1 distribution and basolateral secretion in the retinal pigment epithelium

Santosh Narayan, Anne Marie Brun, Thomas Yorio

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Endothelins are a family of conserved vasoactive peptides that are widely expressed in different biological systems including the eye. In the cell culture model of retinal pigment epithelium, ARPE-19, the synthesis and secretion of endothelin-1 (ET-1) is regulated by cholinergics and TNF-α. In the present study we investigated the expression of ET-1 in RPE in situ, in rat and human eyes. Additionally, we have employed the human retinal pigment epithelial (ARPE-19) cells to delineate the apical and basolateral ET-1 expression and secretion by confocal microscopy and radioimmunoassay, respectively. Our results suggest a possible conservation of ET-1 expression predominantly in the mammalian RPE underlining its importance at this site. Additionally, our results suggest that constitutive ET-1 secretion is predominantly towards the basolateral side in cultured RPE possibly allowing ET-1 to activate its receptors located in the choroidal blood vessels and regulate retinal and choroidal blood flow.

Original language	English
Pages (from-to)	11-19
Number of pages	9
Journal	Experimental eye research
Volume	79
Issue number	1
DOIs	https://doi.org/10.1016/j.exer.2004.03.002
State	Published - Jul 2004

Keywords

endothelin-1
polarity
retinal pigment epithelium

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10.1016/j.exer.2004.03.002

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title = "Endothelin-1 distribution and basolateral secretion in the retinal pigment epithelium",

abstract = "Endothelins are a family of conserved vasoactive peptides that are widely expressed in different biological systems including the eye. In the cell culture model of retinal pigment epithelium, ARPE-19, the synthesis and secretion of endothelin-1 (ET-1) is regulated by cholinergics and TNF-α. In the present study we investigated the expression of ET-1 in RPE in situ, in rat and human eyes. Additionally, we have employed the human retinal pigment epithelial (ARPE-19) cells to delineate the apical and basolateral ET-1 expression and secretion by confocal microscopy and radioimmunoassay, respectively. Our results suggest a possible conservation of ET-1 expression predominantly in the mammalian RPE underlining its importance at this site. Additionally, our results suggest that constitutive ET-1 secretion is predominantly towards the basolateral side in cultured RPE possibly allowing ET-1 to activate its receptors located in the choroidal blood vessels and regulate retinal and choroidal blood flow.",

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AU - Brun, Anne Marie

AU - Yorio, Thomas

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N2 - Endothelins are a family of conserved vasoactive peptides that are widely expressed in different biological systems including the eye. In the cell culture model of retinal pigment epithelium, ARPE-19, the synthesis and secretion of endothelin-1 (ET-1) is regulated by cholinergics and TNF-α. In the present study we investigated the expression of ET-1 in RPE in situ, in rat and human eyes. Additionally, we have employed the human retinal pigment epithelial (ARPE-19) cells to delineate the apical and basolateral ET-1 expression and secretion by confocal microscopy and radioimmunoassay, respectively. Our results suggest a possible conservation of ET-1 expression predominantly in the mammalian RPE underlining its importance at this site. Additionally, our results suggest that constitutive ET-1 secretion is predominantly towards the basolateral side in cultured RPE possibly allowing ET-1 to activate its receptors located in the choroidal blood vessels and regulate retinal and choroidal blood flow.

AB - Endothelins are a family of conserved vasoactive peptides that are widely expressed in different biological systems including the eye. In the cell culture model of retinal pigment epithelium, ARPE-19, the synthesis and secretion of endothelin-1 (ET-1) is regulated by cholinergics and TNF-α. In the present study we investigated the expression of ET-1 in RPE in situ, in rat and human eyes. Additionally, we have employed the human retinal pigment epithelial (ARPE-19) cells to delineate the apical and basolateral ET-1 expression and secretion by confocal microscopy and radioimmunoassay, respectively. Our results suggest a possible conservation of ET-1 expression predominantly in the mammalian RPE underlining its importance at this site. Additionally, our results suggest that constitutive ET-1 secretion is predominantly towards the basolateral side in cultured RPE possibly allowing ET-1 to activate its receptors located in the choroidal blood vessels and regulate retinal and choroidal blood flow.

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KW - polarity

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Endothelin-1 distribution and basolateral secretion in the retinal pigment epithelium

Abstract

Keywords

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