TY - JOUR
T1 - End-to-end distance distributions of flexible molecules from steady state fluorescence energy transfer and quenching-induced changes in the Förster distance
AU - Gryczynski, Ignacy
AU - Wiczk, Wieslaw
AU - Johnson, Michael L.
AU - Lakowicz, Joseph R.
N1 - Funding Information:
Supported by grant GM-35 154 from the National Institutes of Health, with support for instrumentation from the National Science Foundation (DMB-8511065 and DMB-8502835).
PY - 1988/4/15
Y1 - 1988/4/15
N2 - We describe a new method to recover the distribution of distances between two sites on flexible molecules, which requires only steady state measurements of fluorescence intensities. We used collisional quenching of the donor fluorescence to vary the characteristic Förster distance (R0) for energy transfer. The measured transfer efficiencies for each value of R0 provide different samples of the distance distribution. The R0-dependent transfer efficiencies, when analyzed by non-linear least squares, were found to reliably determine the distance distribution, as shown by evaluation of the uncertainties in the distribution and by agreement with the frequency-domain method (Chem. Phys. Letters 138 (1987) 587).
AB - We describe a new method to recover the distribution of distances between two sites on flexible molecules, which requires only steady state measurements of fluorescence intensities. We used collisional quenching of the donor fluorescence to vary the characteristic Förster distance (R0) for energy transfer. The measured transfer efficiencies for each value of R0 provide different samples of the distance distribution. The R0-dependent transfer efficiencies, when analyzed by non-linear least squares, were found to reliably determine the distance distribution, as shown by evaluation of the uncertainties in the distribution and by agreement with the frequency-domain method (Chem. Phys. Letters 138 (1987) 587).
UR - http://www.scopus.com/inward/record.url?scp=0040621112&partnerID=8YFLogxK
U2 - 10.1016/0009-2614(88)80205-7
DO - 10.1016/0009-2614(88)80205-7
M3 - Article
AN - SCOPUS:0040621112
SN - 0009-2614
VL - 145
SP - 439
EP - 446
JO - Chemical Physics Letters
JF - Chemical Physics Letters
IS - 5
ER -