End-to-end diffusion of a flexible bichromophoric molecule observed by intramolecular energy transfer and frequency-domain fluorometry

Joseph R. Lakowicz, Józef Kuśba, Wieslaw Wiczk, Ignacy Gryczynski, Michael L. Johnson

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

We report the first measurement of an end-to-end diffusion coefficient for a flexible molecule using frequency-domain fluorometry. The naphthalene donor was linked to a dansyl acceptor by a polymethylene chain. Frequency-domain intensity decay data were measured for this flexible donor-acceptor pair, in two solvents of modest (48 cP) and low (0.6 cP) viscosity. The data were fit using least-squares criteria with the predicted frequency response for model which includes end-to-end diffusion. Both the initial distance distribution and the diffusion coefficient could be recovered from the data. The resolution of all parameters was improved by global analysis in the low and higher viscosity solvents.

Original languageEnglish
Pages (from-to)319-326
Number of pages8
JournalChemical Physics Letters
Volume173
Issue number4
DOIs
StatePublished - 12 Oct 1990

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