End-to-end diffusion of a flexible bichromophoric molecule observed by intramolecular energy transfer and frequency-domain fluorometry

Joseph R. Lakowicz; Józef Kuśba; Wieslaw Wiczk; Ignacy Gryczynski; Michael L. Johnson

doi:10.1016/0009-2614(90)85277-J

End-to-end diffusion of a flexible bichromophoric molecule observed by intramolecular energy transfer and frequency-domain fluorometry

Joseph R. Lakowicz, Józef Kuśba, Wieslaw Wiczk, Ignacy Gryczynski, Michael L. Johnson

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Abstract

We report the first measurement of an end-to-end diffusion coefficient for a flexible molecule using frequency-domain fluorometry. The naphthalene donor was linked to a dansyl acceptor by a polymethylene chain. Frequency-domain intensity decay data were measured for this flexible donor-acceptor pair, in two solvents of modest (48 cP) and low (0.6 cP) viscosity. The data were fit using least-squares criteria with the predicted frequency response for model which includes end-to-end diffusion. Both the initial distance distribution and the diffusion coefficient could be recovered from the data. The resolution of all parameters was improved by global analysis in the low and higher viscosity solvents.

Original language	English
Pages (from-to)	319-326
Number of pages	8
Journal	Chemical Physics Letters
Volume	173
Issue number	4
DOIs	https://doi.org/10.1016/0009-2614(90)85277-J
State	Published - 12 Oct 1990

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title = "End-to-end diffusion of a flexible bichromophoric molecule observed by intramolecular energy transfer and frequency-domain fluorometry",

abstract = "We report the first measurement of an end-to-end diffusion coefficient for a flexible molecule using frequency-domain fluorometry. The naphthalene donor was linked to a dansyl acceptor by a polymethylene chain. Frequency-domain intensity decay data were measured for this flexible donor-acceptor pair, in two solvents of modest (48 cP) and low (0.6 cP) viscosity. The data were fit using least-squares criteria with the predicted frequency response for model which includes end-to-end diffusion. Both the initial distance distribution and the diffusion coefficient could be recovered from the data. The resolution of all parameters was improved by global analysis in the low and higher viscosity solvents.",

author = "Lakowicz, {Joseph R.} and J{\'o}zef Ku{\'s}ba and Wieslaw Wiczk and Ignacy Gryczynski and Johnson, {Michael L.}",

note = "Funding Information: JK wouldl ike to acknowledgthee P olishA cademy of Sciencefso r partialf inanciahl elpw ithint hep ro-gramC PBP 01.06T. his workw ass upportebdy grant GM 351 54f rom the NationalI nstituteso f Health, with supportf or instrumentatiofnro m NSF Grants DIR 8710401a ndDMB 8502835.",

year = "1990",

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doi = "10.1016/0009-2614(90)85277-J",

language = "English",

volume = "173",

pages = "319--326",

journal = "Chemical Physics Letters",

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T1 - End-to-end diffusion of a flexible bichromophoric molecule observed by intramolecular energy transfer and frequency-domain fluorometry

AU - Lakowicz, Joseph R.

AU - Kuśba, Józef

AU - Wiczk, Wieslaw

AU - Gryczynski, Ignacy

AU - Johnson, Michael L.

N1 - Funding Information: JK wouldl ike to acknowledgthee P olishA cademy of Sciencefso r partialf inanciahl elpw ithint hep ro-gramC PBP 01.06T. his workw ass upportebdy grant GM 351 54f rom the NationalI nstituteso f Health, with supportf or instrumentatiofnro m NSF Grants DIR 8710401a ndDMB 8502835.

PY - 1990/10/12

Y1 - 1990/10/12

N2 - We report the first measurement of an end-to-end diffusion coefficient for a flexible molecule using frequency-domain fluorometry. The naphthalene donor was linked to a dansyl acceptor by a polymethylene chain. Frequency-domain intensity decay data were measured for this flexible donor-acceptor pair, in two solvents of modest (48 cP) and low (0.6 cP) viscosity. The data were fit using least-squares criteria with the predicted frequency response for model which includes end-to-end diffusion. Both the initial distance distribution and the diffusion coefficient could be recovered from the data. The resolution of all parameters was improved by global analysis in the low and higher viscosity solvents.

AB - We report the first measurement of an end-to-end diffusion coefficient for a flexible molecule using frequency-domain fluorometry. The naphthalene donor was linked to a dansyl acceptor by a polymethylene chain. Frequency-domain intensity decay data were measured for this flexible donor-acceptor pair, in two solvents of modest (48 cP) and low (0.6 cP) viscosity. The data were fit using least-squares criteria with the predicted frequency response for model which includes end-to-end diffusion. Both the initial distance distribution and the diffusion coefficient could be recovered from the data. The resolution of all parameters was improved by global analysis in the low and higher viscosity solvents.

UR - http://www.scopus.com/inward/record.url?scp=0001142187&partnerID=8YFLogxK

U2 - 10.1016/0009-2614(90)85277-J

DO - 10.1016/0009-2614(90)85277-J

M3 - Article

AN - SCOPUS:0001142187

SN - 0009-2614

VL - 173

SP - 319

EP - 326

JO - Chemical Physics Letters

JF - Chemical Physics Letters

IS - 4

ER -

End-to-end diffusion of a flexible bichromophoric molecule observed by intramolecular energy transfer and frequency-domain fluorometry

Abstract

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