Emission Spectra of Hemoglobin in the Picosecond Range

Enrico Bucci; Henryk Malak; Clara Fronticelli; Ignacy Gryczynski; Joseph R. Lakowicz

doi:10.1117/12.945387

Emission Spectra of Hemoglobin in the Picosecond Range

Enrico Bucci, Henryk Malak, Clara Fronticelli, Ignacy Gryczynski, Joseph R. Lakowicz

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Abstract

We describe steady state emission spectra and fluorescent decay times from highly purified solutions of human hemoglobin, and the precautions necessary to obtain reliable measurements on the highly quenched intrinsic tryptophan emission. Hemoglobin displays a wide range of decay times (10 ps to 8 ns). The ps component are probably due to Hb itself, and the ns components are probably due to impurities. The ps emission appears to have emission maxima near 320 nm, which is characteristic of tryptophan residues which are shielded from contact with water. The ns components appear to have emission maxima near 340 nm. Our results indicate that the intrinsic emission of Hb can be a useful probe for its functional behavior.

Original language	English
Pages (from-to)	192-199
Number of pages	8
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	909
DOIs	https://doi.org/10.1117/12.945387
State	Published - 24 Jun 1988

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abstract = "We describe steady state emission spectra and fluorescent decay times from highly purified solutions of human hemoglobin, and the precautions necessary to obtain reliable measurements on the highly quenched intrinsic tryptophan emission. Hemoglobin displays a wide range of decay times (10 ps to 8 ns). The ps component are probably due to Hb itself, and the ns components are probably due to impurities. The ps emission appears to have emission maxima near 320 nm, which is characteristic of tryptophan residues which are shielded from contact with water. The ns components appear to have emission maxima near 340 nm. Our results indicate that the intrinsic emission of Hb can be a useful probe for its functional behavior.",

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AU - Bucci, Enrico

AU - Malak, Henryk

AU - Fronticelli, Clara

AU - Gryczynski, Ignacy

AU - Lakowicz, Joseph R.

PY - 1988/6/24

Y1 - 1988/6/24

N2 - We describe steady state emission spectra and fluorescent decay times from highly purified solutions of human hemoglobin, and the precautions necessary to obtain reliable measurements on the highly quenched intrinsic tryptophan emission. Hemoglobin displays a wide range of decay times (10 ps to 8 ns). The ps component are probably due to Hb itself, and the ns components are probably due to impurities. The ps emission appears to have emission maxima near 320 nm, which is characteristic of tryptophan residues which are shielded from contact with water. The ns components appear to have emission maxima near 340 nm. Our results indicate that the intrinsic emission of Hb can be a useful probe for its functional behavior.

AB - We describe steady state emission spectra and fluorescent decay times from highly purified solutions of human hemoglobin, and the precautions necessary to obtain reliable measurements on the highly quenched intrinsic tryptophan emission. Hemoglobin displays a wide range of decay times (10 ps to 8 ns). The ps component are probably due to Hb itself, and the ns components are probably due to impurities. The ps emission appears to have emission maxima near 320 nm, which is characteristic of tryptophan residues which are shielded from contact with water. The ns components appear to have emission maxima near 340 nm. Our results indicate that the intrinsic emission of Hb can be a useful probe for its functional behavior.

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SP - 192

EP - 199

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

ER -

Emission Spectra of Hemoglobin in the Picosecond Range

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