Emission lifetimes of human hemoglobin computed from the atomic coordinates

Zygmunt Gryczynski, Todd Tenenholz, Enrico Bucci

Research output: Chapter in Book/Report/Conference proceedingConference contributionResearchpeer-review

Abstract

Using the Forster equations we have estimated the rate of energy transfer from tryptophans to hemes in hemoglobin. We computed the orientation factors and the consequent transfer rates from the crystallographic coordinates of human oxy- and deoxyhemoglobin. The intrasubunit distances between hemes and tryptophans allow lifetimes between 5 and 15 ps per each ns of tryptophan lifetime. Lifetimes of several hundred ps would be allowed by the intersubunit distances. However, these distances become operative only when one heme per tetramer does not accept transfer. If more than one heme per tetramer does not function as an acceptor, lifetimes of several ns would be allowed.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherPubl by Int Soc for Optical Engineering
Pages550-555
Number of pages6
Volume1640
ISBN (Print)0819407860
StatePublished - 1 Jan 1992
EventTime-Resolved Laser Spectroscopy in Biochemistry III - Los Angeles, CA, USA
Duration: 20 Jan 199222 Jan 1992

Other

OtherTime-Resolved Laser Spectroscopy in Biochemistry III
CityLos Angeles, CA, USA
Period20/01/9222/01/92

Fingerprint

Hemoglobin
hemoglobin
Heme
Energy transfer
tryptophan
Lifetime
Hemoglobins
Tryptophan
life (durability)
oxyhemoglobin
Transfer Factor
Oxyhemoglobins
Energy Transfer
energy transfer
Human

Cite this

Gryczynski, Z., Tenenholz, T., & Bucci, E. (1992). Emission lifetimes of human hemoglobin computed from the atomic coordinates. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 1640, pp. 550-555). Publ by Int Soc for Optical Engineering.
Gryczynski, Zygmunt ; Tenenholz, Todd ; Bucci, Enrico. / Emission lifetimes of human hemoglobin computed from the atomic coordinates. Proceedings of SPIE - The International Society for Optical Engineering. Vol. 1640 Publ by Int Soc for Optical Engineering, 1992. pp. 550-555
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Gryczynski, Z, Tenenholz, T & Bucci, E 1992, Emission lifetimes of human hemoglobin computed from the atomic coordinates. in Proceedings of SPIE - The International Society for Optical Engineering. vol. 1640, Publ by Int Soc for Optical Engineering, pp. 550-555, Time-Resolved Laser Spectroscopy in Biochemistry III, Los Angeles, CA, USA, 20/01/92.

Emission lifetimes of human hemoglobin computed from the atomic coordinates. / Gryczynski, Zygmunt; Tenenholz, Todd; Bucci, Enrico.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 1640 Publ by Int Soc for Optical Engineering, 1992. p. 550-555.

Research output: Chapter in Book/Report/Conference proceedingConference contributionResearchpeer-review

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AB - Using the Forster equations we have estimated the rate of energy transfer from tryptophans to hemes in hemoglobin. We computed the orientation factors and the consequent transfer rates from the crystallographic coordinates of human oxy- and deoxyhemoglobin. The intrasubunit distances between hemes and tryptophans allow lifetimes between 5 and 15 ps per each ns of tryptophan lifetime. Lifetimes of several hundred ps would be allowed by the intersubunit distances. However, these distances become operative only when one heme per tetramer does not accept transfer. If more than one heme per tetramer does not function as an acceptor, lifetimes of several ns would be allowed.

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Gryczynski Z, Tenenholz T, Bucci E. Emission lifetimes of human hemoglobin computed from the atomic coordinates. In Proceedings of SPIE - The International Society for Optical Engineering. Vol. 1640. Publ by Int Soc for Optical Engineering. 1992. p. 550-555