Emission lifetimes of human hemoglobin computed from the atomic coordinates

Zygmunt Gryczynski, Todd Tenenholz, Enrico Bucci

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Using the Forster equations we have estimated the rate of energy transfer from tryptophans to hemes in hemoglobin. We computed the orientation factors and the consequent transfer rates from the crystallographic coordinates of human oxy- and deoxyhemoglobin. The intrasubunit distances between hemes and tryptophans allow lifetimes between 5 and 15 ps per each ns of tryptophan lifetime. Lifetimes of several hundred ps would be allowed by the intersubunit distances. However, these distances become operative only when one heme per tetramer does not accept transfer. If more than one heme per tetramer does not function as an acceptor, lifetimes of several ns would be allowed.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherPubl by Int Soc for Optical Engineering
Pages550-555
Number of pages6
ISBN (Print)0819407860
StatePublished - 1 Jan 1992
EventTime-Resolved Laser Spectroscopy in Biochemistry III - Los Angeles, CA, USA
Duration: 20 Jan 199222 Jan 1992

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume1640
ISSN (Print)0277-786X

Other

OtherTime-Resolved Laser Spectroscopy in Biochemistry III
CityLos Angeles, CA, USA
Period20/01/9222/01/92

Fingerprint Dive into the research topics of 'Emission lifetimes of human hemoglobin computed from the atomic coordinates'. Together they form a unique fingerprint.

  • Cite this

    Gryczynski, Z., Tenenholz, T., & Bucci, E. (1992). Emission lifetimes of human hemoglobin computed from the atomic coordinates. In Proceedings of SPIE - The International Society for Optical Engineering (pp. 550-555). (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 1640). Publ by Int Soc for Optical Engineering.