Embolic middle cerebral artery occlusion model using thrombin and fibrinogen composed clots in rat

Ming Ren, Zi Jing Lin, Hai Qian, Gourav Roy Choudhury, Ran Liu, Hanli Liu, Shaohua Yang

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)

Abstract

Ischemic stroke accounts for over 80% in total human stroke which mostly affect middle cerebral artery (MCA) territory. Embolic stroke models induced by injection of homologous clots into the internal carotid artery and MCA closely mimic human stroke and have been commonly used in stroke research. Studies indicate that the size and composition of clots are critical for the reproducibility of the stroke model. In the present study, we modified the homologous clots formation by addition of thrombin and fibrinogen which produced even distribution of fibrin with tight cross linkage of red blood cells. We optimized the embolic MCA occlusion model in rats using different size of the mixed clots. A precise lodgment of the clots at the MCA bifurcation and highly reproducible ischemic lesion in the MCA territory were demonstrated in the embolic MCA occlusion model induced by injection of 10 pieces of 1-mm long mixed clots made in PE-60 catheter. We further tested the effect of recombinant tissue plasminogen activator (rtPA) in this embolic MCA occlusion model. rtPA induced thrombolysis, improved neurological outcome, and significantly reduced ischemic lesion volume when administered at 1. h after embolism as compared with control. In summary, we have established a reproducible embolic MCA occlusion model using clots made of homologous blood, thrombin and fibrinogen. The mixed clots enable precise lodgment at the MCA bifurcation which is responsive to thrombolytic therapy of rtPA.

Original languageEnglish
Pages (from-to)296-304
Number of pages9
JournalJournal of Neuroscience Methods
Volume211
Issue number2
DOIs
StatePublished - 15 Nov 2012

Fingerprint

Middle Cerebral Artery Infarction
Thrombin
Fibrinogen
Middle Cerebral Artery
Stroke
Tissue Plasminogen Activator
Injections
Thrombolytic Therapy
Internal Carotid Artery
Embolism
Fibrin
Catheters
Erythrocytes
Research

Keywords

  • Embolism
  • Ischemia
  • Middle cerebral artery
  • Recombinant tissue plasminogen activator
  • Stroke

Cite this

Ren, Ming ; Lin, Zi Jing ; Qian, Hai ; Choudhury, Gourav Roy ; Liu, Ran ; Liu, Hanli ; Yang, Shaohua. / Embolic middle cerebral artery occlusion model using thrombin and fibrinogen composed clots in rat. In: Journal of Neuroscience Methods. 2012 ; Vol. 211, No. 2. pp. 296-304.
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abstract = "Ischemic stroke accounts for over 80{\%} in total human stroke which mostly affect middle cerebral artery (MCA) territory. Embolic stroke models induced by injection of homologous clots into the internal carotid artery and MCA closely mimic human stroke and have been commonly used in stroke research. Studies indicate that the size and composition of clots are critical for the reproducibility of the stroke model. In the present study, we modified the homologous clots formation by addition of thrombin and fibrinogen which produced even distribution of fibrin with tight cross linkage of red blood cells. We optimized the embolic MCA occlusion model in rats using different size of the mixed clots. A precise lodgment of the clots at the MCA bifurcation and highly reproducible ischemic lesion in the MCA territory were demonstrated in the embolic MCA occlusion model induced by injection of 10 pieces of 1-mm long mixed clots made in PE-60 catheter. We further tested the effect of recombinant tissue plasminogen activator (rtPA) in this embolic MCA occlusion model. rtPA induced thrombolysis, improved neurological outcome, and significantly reduced ischemic lesion volume when administered at 1. h after embolism as compared with control. In summary, we have established a reproducible embolic MCA occlusion model using clots made of homologous blood, thrombin and fibrinogen. The mixed clots enable precise lodgment at the MCA bifurcation which is responsive to thrombolytic therapy of rtPA.",
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Embolic middle cerebral artery occlusion model using thrombin and fibrinogen composed clots in rat. / Ren, Ming; Lin, Zi Jing; Qian, Hai; Choudhury, Gourav Roy; Liu, Ran; Liu, Hanli; Yang, Shaohua.

In: Journal of Neuroscience Methods, Vol. 211, No. 2, 15.11.2012, p. 296-304.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Lin, Zi Jing

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AU - Liu, Hanli

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