Elevated glucose in vivo and in vitro adversely alters prostaglandin generation in rat aortas and platelets

M. A. Valentovic, William Charles Lubawy

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

An imbalance in prostacyclin (PGI2) and thromboxane (TXA2) generation from arachidonic acid (AA) may contribute to the marked increase in susceptibility to cardiovascular disease seen in diabetics. Rats made diabetic with streptozocin, and subsequently treated with saline or insulin, yielded aortic rings that synthesized decreasing amounts of PGI2 and platelets that generated increasing amounts of TXA2 in proportion to the degree of hyperglycemia. These alterations in AA metabolism were mimicked by incubating aortic rings or platelets from normal rats in buffer containing elevated glucose concentrations. Platelets incubated in elevated glucose displayed shorter times to maximal aggregation and- higher percent maximal aggregation. Incubation of tissue in a buffer made hyperosmotic with mannitol had no effect on PGI2 or TXA2 formation, or platelet aggregation. These data suggest that hyperglycemia is involved in the PGI2/TXA2 imbalance and platelet abnormalities seen in diabetes, and reinforces the importance of rigid control of blood glucose as an approach to minimizing the incidence of diabetic cardiovascular complications.

Original languageEnglish
Pages (from-to)271-277
Number of pages7
JournalProstaglandins, Leukotrienes and Medicine
Volume19
Issue number3
DOIs
StatePublished - 1 Jan 1985

Fingerprint

Epoprostenol
Platelets
Prostaglandins
Aorta
Rats
Blood Platelets
Glucose
Agglomeration
Arachidonic Acid
Hyperglycemia
Buffers
Thromboxanes
Mannitol
Diabetes Complications
Streptozocin
Medical problems
Platelet Aggregation
Metabolism
Blood Glucose
Cardiovascular Diseases

Cite this

@article{015b7eafd8a949f39216e9c6e02a8871,
title = "Elevated glucose in vivo and in vitro adversely alters prostaglandin generation in rat aortas and platelets",
abstract = "An imbalance in prostacyclin (PGI2) and thromboxane (TXA2) generation from arachidonic acid (AA) may contribute to the marked increase in susceptibility to cardiovascular disease seen in diabetics. Rats made diabetic with streptozocin, and subsequently treated with saline or insulin, yielded aortic rings that synthesized decreasing amounts of PGI2 and platelets that generated increasing amounts of TXA2 in proportion to the degree of hyperglycemia. These alterations in AA metabolism were mimicked by incubating aortic rings or platelets from normal rats in buffer containing elevated glucose concentrations. Platelets incubated in elevated glucose displayed shorter times to maximal aggregation and- higher percent maximal aggregation. Incubation of tissue in a buffer made hyperosmotic with mannitol had no effect on PGI2 or TXA2 formation, or platelet aggregation. These data suggest that hyperglycemia is involved in the PGI2/TXA2 imbalance and platelet abnormalities seen in diabetes, and reinforces the importance of rigid control of blood glucose as an approach to minimizing the incidence of diabetic cardiovascular complications.",
author = "Valentovic, {M. A.} and Lubawy, {William Charles}",
year = "1985",
month = "1",
day = "1",
doi = "10.1016/0262-1746(85)90140-4",
language = "English",
volume = "19",
pages = "271--277",
journal = "Prostaglandins, Leukotrienes and Medicine",
issn = "0262-1746",
publisher = "Churchill Livingstone",
number = "3",

}

Elevated glucose in vivo and in vitro adversely alters prostaglandin generation in rat aortas and platelets. / Valentovic, M. A.; Lubawy, William Charles.

In: Prostaglandins, Leukotrienes and Medicine, Vol. 19, No. 3, 01.01.1985, p. 271-277.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Elevated glucose in vivo and in vitro adversely alters prostaglandin generation in rat aortas and platelets

AU - Valentovic, M. A.

AU - Lubawy, William Charles

PY - 1985/1/1

Y1 - 1985/1/1

N2 - An imbalance in prostacyclin (PGI2) and thromboxane (TXA2) generation from arachidonic acid (AA) may contribute to the marked increase in susceptibility to cardiovascular disease seen in diabetics. Rats made diabetic with streptozocin, and subsequently treated with saline or insulin, yielded aortic rings that synthesized decreasing amounts of PGI2 and platelets that generated increasing amounts of TXA2 in proportion to the degree of hyperglycemia. These alterations in AA metabolism were mimicked by incubating aortic rings or platelets from normal rats in buffer containing elevated glucose concentrations. Platelets incubated in elevated glucose displayed shorter times to maximal aggregation and- higher percent maximal aggregation. Incubation of tissue in a buffer made hyperosmotic with mannitol had no effect on PGI2 or TXA2 formation, or platelet aggregation. These data suggest that hyperglycemia is involved in the PGI2/TXA2 imbalance and platelet abnormalities seen in diabetes, and reinforces the importance of rigid control of blood glucose as an approach to minimizing the incidence of diabetic cardiovascular complications.

AB - An imbalance in prostacyclin (PGI2) and thromboxane (TXA2) generation from arachidonic acid (AA) may contribute to the marked increase in susceptibility to cardiovascular disease seen in diabetics. Rats made diabetic with streptozocin, and subsequently treated with saline or insulin, yielded aortic rings that synthesized decreasing amounts of PGI2 and platelets that generated increasing amounts of TXA2 in proportion to the degree of hyperglycemia. These alterations in AA metabolism were mimicked by incubating aortic rings or platelets from normal rats in buffer containing elevated glucose concentrations. Platelets incubated in elevated glucose displayed shorter times to maximal aggregation and- higher percent maximal aggregation. Incubation of tissue in a buffer made hyperosmotic with mannitol had no effect on PGI2 or TXA2 formation, or platelet aggregation. These data suggest that hyperglycemia is involved in the PGI2/TXA2 imbalance and platelet abnormalities seen in diabetes, and reinforces the importance of rigid control of blood glucose as an approach to minimizing the incidence of diabetic cardiovascular complications.

UR - http://www.scopus.com/inward/record.url?scp=0022414194&partnerID=8YFLogxK

U2 - 10.1016/0262-1746(85)90140-4

DO - 10.1016/0262-1746(85)90140-4

M3 - Article

C2 - 3903777

AN - SCOPUS:0022414194

VL - 19

SP - 271

EP - 277

JO - Prostaglandins, Leukotrienes and Medicine

JF - Prostaglandins, Leukotrienes and Medicine

SN - 0262-1746

IS - 3

ER -