Electrospray ionization mass spectrometry of cyclodextrin complexes of amino acids and peptides

Laszlo Prokai; R. Ramanathan; J. Nawrocki; J. Eyler

doi:10.1007/BF01041549

Electrospray ionization mass spectrometry of cyclodextrin complexes of amino acids and peptides

Laszlo Prokai, R. Ramanathan, J. Nawrocki, J. Eyler

Institute for Healthy Aging

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20 Scopus citations

Abstract

Appropriate complex-forming side-chains of peptides and proteins can bind cyclodextrins. We have characterized interactions of amino acids with cyclodextrins by electrospray ionization mass spectrometry. The stoichiometry of various α-cyclodextrin/protonated tryptophan complexes sampled from solution to the gas phase by ESI was revealed by taking advantage of the extended mass range and high mass resolution of a Fourier-transform ion cyclotron resonance instrument. Binding of β-cyclodextrin to recombinant human insulin has been demonstrated.

Original language	English
Pages (from-to)	117-120
Number of pages	4
Journal	Journal of Inclusion Phenomena and Molecular Recognition in Chemistry
Volume	25
Issue number	1-3
DOIs	https://doi.org/10.1007/BF01041549
State	Published - 1 Jan 1996

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abstract = "Appropriate complex-forming side-chains of peptides and proteins can bind cyclodextrins. We have characterized interactions of amino acids with cyclodextrins by electrospray ionization mass spectrometry. The stoichiometry of various α-cyclodextrin/protonated tryptophan complexes sampled from solution to the gas phase by ESI was revealed by taking advantage of the extended mass range and high mass resolution of a Fourier-transform ion cyclotron resonance instrument. Binding of β-cyclodextrin to recombinant human insulin has been demonstrated.",

author = "Laszlo Prokai and R. Ramanathan and J. Nawrocki and J. Eyler",

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AU - Prokai, Laszlo

AU - Ramanathan, R.

AU - Nawrocki, J.

AU - Eyler, J.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - Appropriate complex-forming side-chains of peptides and proteins can bind cyclodextrins. We have characterized interactions of amino acids with cyclodextrins by electrospray ionization mass spectrometry. The stoichiometry of various α-cyclodextrin/protonated tryptophan complexes sampled from solution to the gas phase by ESI was revealed by taking advantage of the extended mass range and high mass resolution of a Fourier-transform ion cyclotron resonance instrument. Binding of β-cyclodextrin to recombinant human insulin has been demonstrated.

AB - Appropriate complex-forming side-chains of peptides and proteins can bind cyclodextrins. We have characterized interactions of amino acids with cyclodextrins by electrospray ionization mass spectrometry. The stoichiometry of various α-cyclodextrin/protonated tryptophan complexes sampled from solution to the gas phase by ESI was revealed by taking advantage of the extended mass range and high mass resolution of a Fourier-transform ion cyclotron resonance instrument. Binding of β-cyclodextrin to recombinant human insulin has been demonstrated.

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SN - 0923-0750

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JO - Journal of Inclusion Phenomena and Molecular Recognition in Chemistry

JF - Journal of Inclusion Phenomena and Molecular Recognition in Chemistry

IS - 1-3

ER -

Electrospray ionization mass spectrometry of cyclodextrin complexes of amino acids and peptides

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