Electrophysiological neurodifferentiation of subventricular zone-derived precursor cells following stroke

Bin Lai, Xiao Ou Mao, Lin Xie, Kunlin Jin, David A. Greenberg

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Stroke in rodents is associated with increased neurogenesis and the migration of newborn neurons to sites of brain ischemia, where they may participate in repair and recovery. To determine if neurogenesis following stroke yields functional new neurons, we labeled neuronal precursors in the mouse subventricular zone (SVZ) with a lentivirus-green fluorescent protein vector, produced stroke by occluding the middle cerebral artery, and detected newborn neurons 8 weeks later by fluorescence microscopy. Patch-clamp studies on fluorescent neurons in the cortical region surrounding infarction showed tetrodotoxin-sensitive Na+ action potentials and spontaneous excitatory post-synaptic currents, suggesting that ischemia led to functional neurogenesis with synaptic integration. These findings support the hypothesis that enhancing endogenous neurogenesis after stroke might have therapeutic benefit.

Original languageEnglish
Pages (from-to)305-308
Number of pages4
JournalNeuroscience letters
Volume442
Issue number3
DOIs
StatePublished - 19 Sep 2008

Keywords

  • Action potential
  • Differentiation
  • Ischemia
  • Neurogenesis
  • Stroke
  • Subventricular zone

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